Job ID = 1295315 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 67,994,326 reads read : 67,994,326 reads written : 67,994,326 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:24 67994326 reads; of these: 67994326 (100.00%) were unpaired; of these: 9958744 (14.65%) aligned 0 times 46700298 (68.68%) aligned exactly 1 time 11335284 (16.67%) aligned >1 times 85.35% overall alignment rate Time searching: 00:20:24 Overall time: 00:20:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 22298160 / 58035582 = 0.3842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 14:12:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:33: 1000000 INFO @ Mon, 03 Jun 2019 14:12:33: 1000000 INFO @ Mon, 03 Jun 2019 14:12:34: 1000000 INFO @ Mon, 03 Jun 2019 14:12:41: 2000000 INFO @ Mon, 03 Jun 2019 14:12:41: 2000000 INFO @ Mon, 03 Jun 2019 14:12:44: 2000000 INFO @ Mon, 03 Jun 2019 14:12:49: 3000000 INFO @ Mon, 03 Jun 2019 14:12:49: 3000000 INFO @ Mon, 03 Jun 2019 14:12:53: 3000000 INFO @ Mon, 03 Jun 2019 14:12:57: 4000000 INFO @ Mon, 03 Jun 2019 14:12:58: 4000000 INFO @ Mon, 03 Jun 2019 14:13:02: 4000000 INFO @ Mon, 03 Jun 2019 14:13:05: 5000000 INFO @ Mon, 03 Jun 2019 14:13:06: 5000000 INFO @ Mon, 03 Jun 2019 14:13:11: 5000000 INFO @ Mon, 03 Jun 2019 14:13:13: 6000000 INFO @ Mon, 03 Jun 2019 14:13:14: 6000000 INFO @ Mon, 03 Jun 2019 14:13:20: 6000000 INFO @ Mon, 03 Jun 2019 14:13:21: 7000000 INFO @ Mon, 03 Jun 2019 14:13:22: 7000000 INFO @ Mon, 03 Jun 2019 14:13:29: 7000000 INFO @ Mon, 03 Jun 2019 14:13:29: 8000000 INFO @ Mon, 03 Jun 2019 14:13:30: 8000000 INFO @ Mon, 03 Jun 2019 14:13:38: 9000000 INFO @ Mon, 03 Jun 2019 14:13:38: 9000000 INFO @ Mon, 03 Jun 2019 14:13:38: 8000000 INFO @ Mon, 03 Jun 2019 14:13:46: 10000000 INFO @ Mon, 03 Jun 2019 14:13:46: 10000000 INFO @ Mon, 03 Jun 2019 14:13:47: 9000000 INFO @ Mon, 03 Jun 2019 14:13:54: 11000000 INFO @ Mon, 03 Jun 2019 14:13:54: 11000000 INFO @ Mon, 03 Jun 2019 14:13:56: 10000000 INFO @ Mon, 03 Jun 2019 14:14:02: 12000000 INFO @ Mon, 03 Jun 2019 14:14:02: 12000000 INFO @ Mon, 03 Jun 2019 14:14:04: 11000000 INFO @ Mon, 03 Jun 2019 14:14:09: 13000000 INFO @ Mon, 03 Jun 2019 14:14:09: 13000000 INFO @ Mon, 03 Jun 2019 14:14:13: 12000000 INFO @ Mon, 03 Jun 2019 14:14:16: 14000000 INFO @ Mon, 03 Jun 2019 14:14:17: 14000000 INFO @ Mon, 03 Jun 2019 14:14:22: 13000000 INFO @ Mon, 03 Jun 2019 14:14:24: 15000000 INFO @ Mon, 03 Jun 2019 14:14:25: 15000000 INFO @ Mon, 03 Jun 2019 14:14:31: 14000000 INFO @ Mon, 03 Jun 2019 14:14:32: 16000000 INFO @ Mon, 03 Jun 2019 14:14:33: 16000000 INFO @ Mon, 03 Jun 2019 14:14:40: 17000000 INFO @ Mon, 03 Jun 2019 14:14:40: 15000000 INFO @ Mon, 03 Jun 2019 14:14:41: 17000000 INFO @ Mon, 03 Jun 2019 14:14:48: 18000000 INFO @ Mon, 03 Jun 2019 14:14:49: 18000000 INFO @ Mon, 03 Jun 2019 14:14:50: 16000000 INFO @ Mon, 03 Jun 2019 14:14:56: 19000000 INFO @ Mon, 03 Jun 2019 14:14:57: 19000000 INFO @ Mon, 03 Jun 2019 14:14:59: 17000000 INFO @ Mon, 03 Jun 2019 14:15:04: 20000000 INFO @ Mon, 03 Jun 2019 14:15:06: 20000000 INFO @ Mon, 03 Jun 2019 14:15:08: 18000000 INFO @ Mon, 03 Jun 2019 14:15:13: 21000000 INFO @ Mon, 03 Jun 2019 14:15:14: 21000000 INFO @ Mon, 03 Jun 2019 14:15:17: 19000000 INFO @ Mon, 03 Jun 2019 14:15:21: 22000000 INFO @ Mon, 03 Jun 2019 14:15:23: 22000000 INFO @ Mon, 03 Jun 2019 14:15:26: 20000000 INFO @ Mon, 03 Jun 2019 14:15:30: 23000000 INFO @ Mon, 03 Jun 2019 14:15:31: 23000000 INFO @ Mon, 03 Jun 2019 14:15:35: 21000000 INFO @ Mon, 03 Jun 2019 14:15:38: 24000000 INFO @ Mon, 03 Jun 2019 14:15:39: 24000000 INFO @ Mon, 03 Jun 2019 14:15:43: 22000000 INFO @ Mon, 03 Jun 2019 14:15:46: 25000000 INFO @ Mon, 03 Jun 2019 14:15:48: 25000000 INFO @ Mon, 03 Jun 2019 14:15:52: 23000000 INFO @ Mon, 03 Jun 2019 14:15:55: 26000000 INFO @ Mon, 03 Jun 2019 14:15:56: 26000000 INFO @ Mon, 03 Jun 2019 14:16:01: 24000000 INFO @ Mon, 03 Jun 2019 14:16:04: 27000000 INFO @ Mon, 03 Jun 2019 14:16:05: 27000000 INFO @ Mon, 03 Jun 2019 14:16:10: 25000000 INFO @ Mon, 03 Jun 2019 14:16:13: 28000000 INFO @ Mon, 03 Jun 2019 14:16:14: 28000000 INFO @ Mon, 03 Jun 2019 14:16:19: 26000000 INFO @ Mon, 03 Jun 2019 14:16:21: 29000000 INFO @ Mon, 03 Jun 2019 14:16:23: 29000000 INFO @ Mon, 03 Jun 2019 14:16:29: 27000000 INFO @ Mon, 03 Jun 2019 14:16:30: 30000000 INFO @ Mon, 03 Jun 2019 14:16:31: 30000000 INFO @ Mon, 03 Jun 2019 14:16:38: 28000000 INFO @ Mon, 03 Jun 2019 14:16:38: 31000000 INFO @ Mon, 03 Jun 2019 14:16:40: 31000000 INFO @ Mon, 03 Jun 2019 14:16:47: 32000000 INFO @ Mon, 03 Jun 2019 14:16:47: 29000000 INFO @ Mon, 03 Jun 2019 14:16:48: 32000000 INFO @ Mon, 03 Jun 2019 14:16:55: 33000000 INFO @ Mon, 03 Jun 2019 14:16:57: 33000000 INFO @ Mon, 03 Jun 2019 14:16:57: 30000000 INFO @ Mon, 03 Jun 2019 14:17:04: 34000000 INFO @ Mon, 03 Jun 2019 14:17:05: 34000000 INFO @ Mon, 03 Jun 2019 14:17:06: 31000000 INFO @ Mon, 03 Jun 2019 14:17:12: 35000000 INFO @ Mon, 03 Jun 2019 14:17:14: 35000000 INFO @ Mon, 03 Jun 2019 14:17:15: 32000000 INFO @ Mon, 03 Jun 2019 14:17:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 14:17:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 14:17:19: #1 total tags in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:17:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:17:20: #1 tags after filtering in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:17:20: #1 finished! INFO @ Mon, 03 Jun 2019 14:17:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:17:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:17:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 14:17:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 14:17:20: #1 total tags in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:17:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:17:21: #1 tags after filtering in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:17:21: #1 finished! INFO @ Mon, 03 Jun 2019 14:17:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:17:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:17:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 14:17:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:17:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:17:23: 33000000 INFO @ Mon, 03 Jun 2019 14:17:24: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 14:17:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:17:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:17:32: 34000000 INFO @ Mon, 03 Jun 2019 14:17:40: 35000000 INFO @ Mon, 03 Jun 2019 14:17:47: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 14:17:47: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 14:17:47: #1 total tags in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:17:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:17:48: #1 tags after filtering in treatment: 35737422 INFO @ Mon, 03 Jun 2019 14:17:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:17:48: #1 finished! INFO @ Mon, 03 Jun 2019 14:17:48: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:17:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:17:51: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 14:17:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:17:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335487/SRX335487.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。