Job ID = 1295225 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,014,950 reads read : 21,014,950 reads written : 21,014,950 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:09 21014950 reads; of these: 21014950 (100.00%) were unpaired; of these: 1185144 (5.64%) aligned 0 times 14968684 (71.23%) aligned exactly 1 time 4861122 (23.13%) aligned >1 times 94.36% overall alignment rate Time searching: 00:10:09 Overall time: 00:10:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2683200 / 19829806 = 0.1353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:13:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:13:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:13:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:13:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:13:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:13:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:13:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:13:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:13:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:13:24: 1000000 INFO @ Mon, 03 Jun 2019 13:13:25: 1000000 INFO @ Mon, 03 Jun 2019 13:13:25: 1000000 INFO @ Mon, 03 Jun 2019 13:13:34: 2000000 INFO @ Mon, 03 Jun 2019 13:13:36: 2000000 INFO @ Mon, 03 Jun 2019 13:13:37: 2000000 INFO @ Mon, 03 Jun 2019 13:13:45: 3000000 INFO @ Mon, 03 Jun 2019 13:13:48: 3000000 INFO @ Mon, 03 Jun 2019 13:13:50: 3000000 INFO @ Mon, 03 Jun 2019 13:13:56: 4000000 INFO @ Mon, 03 Jun 2019 13:14:00: 4000000 INFO @ Mon, 03 Jun 2019 13:14:01: 4000000 INFO @ Mon, 03 Jun 2019 13:14:07: 5000000 INFO @ Mon, 03 Jun 2019 13:14:11: 5000000 INFO @ Mon, 03 Jun 2019 13:14:13: 5000000 INFO @ Mon, 03 Jun 2019 13:14:17: 6000000 INFO @ Mon, 03 Jun 2019 13:14:23: 6000000 INFO @ Mon, 03 Jun 2019 13:14:25: 6000000 INFO @ Mon, 03 Jun 2019 13:14:28: 7000000 INFO @ Mon, 03 Jun 2019 13:14:34: 7000000 INFO @ Mon, 03 Jun 2019 13:14:37: 7000000 INFO @ Mon, 03 Jun 2019 13:14:39: 8000000 INFO @ Mon, 03 Jun 2019 13:14:46: 8000000 INFO @ Mon, 03 Jun 2019 13:14:48: 8000000 INFO @ Mon, 03 Jun 2019 13:14:49: 9000000 INFO @ Mon, 03 Jun 2019 13:14:58: 9000000 INFO @ Mon, 03 Jun 2019 13:15:00: 10000000 INFO @ Mon, 03 Jun 2019 13:15:00: 9000000 INFO @ Mon, 03 Jun 2019 13:15:09: 10000000 INFO @ Mon, 03 Jun 2019 13:15:10: 11000000 INFO @ Mon, 03 Jun 2019 13:15:12: 10000000 INFO @ Mon, 03 Jun 2019 13:15:20: 12000000 INFO @ Mon, 03 Jun 2019 13:15:21: 11000000 INFO @ Mon, 03 Jun 2019 13:15:23: 11000000 INFO @ Mon, 03 Jun 2019 13:15:31: 13000000 INFO @ Mon, 03 Jun 2019 13:15:33: 12000000 INFO @ Mon, 03 Jun 2019 13:15:35: 12000000 INFO @ Mon, 03 Jun 2019 13:15:42: 14000000 INFO @ Mon, 03 Jun 2019 13:15:44: 13000000 INFO @ Mon, 03 Jun 2019 13:15:47: 13000000 INFO @ Mon, 03 Jun 2019 13:15:53: 15000000 INFO @ Mon, 03 Jun 2019 13:15:56: 14000000 INFO @ Mon, 03 Jun 2019 13:15:59: 14000000 INFO @ Mon, 03 Jun 2019 13:16:03: 16000000 INFO @ Mon, 03 Jun 2019 13:16:08: 15000000 INFO @ Mon, 03 Jun 2019 13:16:11: 15000000 INFO @ Mon, 03 Jun 2019 13:16:14: 17000000 INFO @ Mon, 03 Jun 2019 13:16:16: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:16:16: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:16:16: #1 total tags in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:16: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:16:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:16:16: #1 tags after filtering in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:16:16: #1 finished! INFO @ Mon, 03 Jun 2019 13:16:16: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:16:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:16:18: #2 number of paired peaks: 67 WARNING @ Mon, 03 Jun 2019 13:16:18: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:16:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:16:20: 16000000 INFO @ Mon, 03 Jun 2019 13:16:23: 16000000 INFO @ Mon, 03 Jun 2019 13:16:32: 17000000 INFO @ Mon, 03 Jun 2019 13:16:33: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:16:33: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:16:33: #1 total tags in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:16:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:16:34: #1 tags after filtering in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:16:34: #1 finished! INFO @ Mon, 03 Jun 2019 13:16:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:16:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:16:35: #2 number of paired peaks: 67 WARNING @ Mon, 03 Jun 2019 13:16:35: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:16:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:16:35: 17000000 INFO @ Mon, 03 Jun 2019 13:16:37: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:16:37: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:16:37: #1 total tags in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:37: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:16:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:16:38: #1 tags after filtering in treatment: 17146606 INFO @ Mon, 03 Jun 2019 13:16:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:16:38: #1 finished! INFO @ Mon, 03 Jun 2019 13:16:38: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:16:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:16:39: #2 number of paired peaks: 67 WARNING @ Mon, 03 Jun 2019 13:16:39: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:16:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331398/SRX331398.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。