Job ID = 10320347 sra ファイルのダウンロード中... Completed: 1742269K bytes transferred in 182 seconds (78270K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 52070511 spots for /home/okishinya/chipatlas/results/dm3/SRX3270984/SRR6159395.sra Written 52070511 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:47 52070511 reads; of these: 52070511 (100.00%) were unpaired; of these: 1261235 (2.42%) aligned 0 times 29834829 (57.30%) aligned exactly 1 time 20974447 (40.28%) aligned >1 times 97.58% overall alignment rate Time searching: 00:27:47 Overall time: 00:27:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10673216 / 50809276 = 0.2101 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 11 Jan 2018 02:51:45: # Command line: callpeak -t SRX3270984.bam -f BAM -g dm -n SRX3270984.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3270984.20 # format = BAM # ChIP-seq file = ['SRX3270984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:51:45: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:51:45: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:51:45: # Command line: callpeak -t SRX3270984.bam -f BAM -g dm -n SRX3270984.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3270984.10 # format = BAM # ChIP-seq file = ['SRX3270984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:51:45: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:51:45: # Command line: callpeak -t SRX3270984.bam -f BAM -g dm -n SRX3270984.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3270984.05 # format = BAM # ChIP-seq file = ['SRX3270984.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:51:45: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:51:45: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:51:45: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:51:52: 1000000 INFO @ Thu, 11 Jan 2018 02:51:52: 1000000 INFO @ Thu, 11 Jan 2018 02:51:52: 1000000 INFO @ Thu, 11 Jan 2018 02:51:58: 2000000 INFO @ Thu, 11 Jan 2018 02:51:59: 2000000 INFO @ Thu, 11 Jan 2018 02:51:59: 2000000 INFO @ Thu, 11 Jan 2018 02:52:05: 3000000 INFO @ Thu, 11 Jan 2018 02:52:06: 3000000 INFO @ Thu, 11 Jan 2018 02:52:06: 3000000 INFO @ Thu, 11 Jan 2018 02:52:11: 4000000 INFO @ Thu, 11 Jan 2018 02:52:13: 4000000 INFO @ Thu, 11 Jan 2018 02:52:13: 4000000 INFO @ Thu, 11 Jan 2018 02:52:17: 5000000 INFO @ Thu, 11 Jan 2018 02:52:20: 5000000 INFO @ Thu, 11 Jan 2018 02:52:21: 5000000 INFO @ Thu, 11 Jan 2018 02:52:23: 6000000 INFO @ Thu, 11 Jan 2018 02:52:26: 6000000 INFO @ Thu, 11 Jan 2018 02:52:28: 6000000 INFO @ Thu, 11 Jan 2018 02:52:30: 7000000 INFO @ Thu, 11 Jan 2018 02:52:33: 7000000 INFO @ Thu, 11 Jan 2018 02:52:35: 7000000 INFO @ Thu, 11 Jan 2018 02:52:36: 8000000 INFO @ Thu, 11 Jan 2018 02:52:40: 8000000 INFO @ Thu, 11 Jan 2018 02:52:42: 8000000 INFO @ Thu, 11 Jan 2018 02:52:42: 9000000 INFO @ Thu, 11 Jan 2018 02:52:47: 9000000 INFO @ Thu, 11 Jan 2018 02:52:49: 10000000 INFO @ Thu, 11 Jan 2018 02:52:49: 9000000 INFO @ Thu, 11 Jan 2018 02:52:54: 10000000 INFO @ Thu, 11 Jan 2018 02:52:55: 11000000 INFO @ Thu, 11 Jan 2018 02:52:57: 10000000 INFO @ Thu, 11 Jan 2018 02:53:01: 12000000 INFO @ Thu, 11 Jan 2018 02:53:01: 11000000 INFO @ Thu, 11 Jan 2018 02:53:04: 11000000 INFO @ Thu, 11 Jan 2018 02:53:07: 13000000 INFO @ Thu, 11 Jan 2018 02:53:08: 12000000 INFO @ Thu, 11 Jan 2018 02:53:11: 12000000 INFO @ Thu, 11 Jan 2018 02:53:14: 14000000 INFO @ Thu, 11 Jan 2018 02:53:15: 13000000 INFO @ Thu, 11 Jan 2018 02:53:18: 13000000 INFO @ Thu, 11 Jan 2018 02:53:20: 15000000 INFO @ Thu, 11 Jan 2018 02:53:22: 14000000 INFO @ Thu, 11 Jan 2018 02:53:25: 14000000 INFO @ Thu, 11 Jan 2018 02:53:26: 16000000 INFO @ Thu, 11 Jan 2018 02:53:29: 15000000 INFO @ Thu, 11 Jan 2018 02:53:32: 17000000 INFO @ Thu, 11 Jan 2018 02:53:33: 15000000 INFO @ Thu, 11 Jan 2018 02:53:36: 16000000 INFO @ Thu, 11 Jan 2018 02:53:39: 18000000 INFO @ Thu, 11 Jan 2018 02:53:40: 16000000 INFO @ Thu, 11 Jan 2018 02:53:43: 17000000 INFO @ Thu, 11 Jan 2018 02:53:45: 19000000 INFO @ Thu, 11 Jan 2018 02:53:47: 17000000 INFO @ Thu, 11 Jan 2018 02:53:50: 18000000 INFO @ Thu, 11 Jan 2018 02:53:51: 20000000 INFO @ Thu, 11 Jan 2018 02:53:54: 18000000 INFO @ Thu, 11 Jan 2018 02:53:57: 19000000 INFO @ Thu, 11 Jan 2018 02:53:57: 21000000 INFO @ Thu, 11 Jan 2018 02:54:01: 19000000 INFO @ Thu, 11 Jan 2018 02:54:04: 22000000 INFO @ Thu, 11 Jan 2018 02:54:04: 20000000 INFO @ Thu, 11 Jan 2018 02:54:09: 20000000 INFO @ Thu, 11 Jan 2018 02:54:10: 23000000 INFO @ Thu, 11 Jan 2018 02:54:11: 21000000 INFO @ Thu, 11 Jan 2018 02:54:16: 21000000 INFO @ Thu, 11 Jan 2018 02:54:16: 24000000 INFO @ Thu, 11 Jan 2018 02:54:18: 22000000 INFO @ Thu, 11 Jan 2018 02:54:22: 25000000 INFO @ Thu, 11 Jan 2018 02:54:23: 22000000 INFO @ Thu, 11 Jan 2018 02:54:25: 23000000 INFO @ Thu, 11 Jan 2018 02:54:29: 26000000 INFO @ Thu, 11 Jan 2018 02:54:30: 23000000 INFO @ Thu, 11 Jan 2018 02:54:32: 24000000 INFO @ Thu, 11 Jan 2018 02:54:35: 27000000 INFO @ Thu, 11 Jan 2018 02:54:37: 24000000 INFO @ Thu, 11 Jan 2018 02:54:39: 25000000 INFO @ Thu, 11 Jan 2018 02:54:41: 28000000 INFO @ Thu, 11 Jan 2018 02:54:44: 25000000 INFO @ Thu, 11 Jan 2018 02:54:46: 26000000 INFO @ Thu, 11 Jan 2018 02:54:47: 29000000 INFO @ Thu, 11 Jan 2018 02:54:52: 26000000 INFO @ Thu, 11 Jan 2018 02:54:52: 27000000 INFO @ Thu, 11 Jan 2018 02:54:53: 30000000 INFO @ Thu, 11 Jan 2018 02:54:59: 27000000 INFO @ Thu, 11 Jan 2018 02:54:59: 28000000 INFO @ Thu, 11 Jan 2018 02:55:00: 31000000 INFO @ Thu, 11 Jan 2018 02:55:06: 32000000 INFO @ Thu, 11 Jan 2018 02:55:06: 28000000 INFO @ Thu, 11 Jan 2018 02:55:06: 29000000 INFO @ Thu, 11 Jan 2018 02:55:12: 33000000 INFO @ Thu, 11 Jan 2018 02:55:13: 29000000 INFO @ Thu, 11 Jan 2018 02:55:13: 30000000 INFO @ Thu, 11 Jan 2018 02:55:18: 34000000 INFO @ Thu, 11 Jan 2018 02:55:20: 31000000 INFO @ Thu, 11 Jan 2018 02:55:20: 30000000 INFO @ Thu, 11 Jan 2018 02:55:24: 35000000 INFO @ Thu, 11 Jan 2018 02:55:27: 32000000 INFO @ Thu, 11 Jan 2018 02:55:28: 31000000 INFO @ Thu, 11 Jan 2018 02:55:31: 36000000 INFO @ Thu, 11 Jan 2018 02:55:34: 33000000 INFO @ Thu, 11 Jan 2018 02:55:35: 32000000 INFO @ Thu, 11 Jan 2018 02:55:37: 37000000 INFO @ Thu, 11 Jan 2018 02:55:41: 34000000 INFO @ Thu, 11 Jan 2018 02:55:42: 33000000 INFO @ Thu, 11 Jan 2018 02:55:43: 38000000 INFO @ Thu, 11 Jan 2018 02:55:48: 35000000 INFO @ Thu, 11 Jan 2018 02:55:49: 34000000 INFO @ Thu, 11 Jan 2018 02:55:49: 39000000 INFO @ Thu, 11 Jan 2018 02:55:55: 36000000 INFO @ Thu, 11 Jan 2018 02:55:55: 40000000 INFO @ Thu, 11 Jan 2018 02:55:56: 35000000 INFO @ Thu, 11 Jan 2018 02:55:57: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:55:57: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:55:57: #1 total tags in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:55:57: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:55:58: #1 tags after filtering in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:55:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:55:58: #1 finished! INFO @ Thu, 11 Jan 2018 02:55:58: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:55:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:56:00: #2 number of paired peaks: 6 WARNING @ Thu, 11 Jan 2018 02:56:00: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:56:00: Process for pairing-model is terminated! cat: SRX3270984.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3270984.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:56:02: 37000000 INFO @ Thu, 11 Jan 2018 02:56:03: 36000000 INFO @ Thu, 11 Jan 2018 02:56:09: 38000000 INFO @ Thu, 11 Jan 2018 02:56:10: 37000000 INFO @ Thu, 11 Jan 2018 02:56:16: 39000000 INFO @ Thu, 11 Jan 2018 02:56:18: 38000000 INFO @ Thu, 11 Jan 2018 02:56:23: 40000000 INFO @ Thu, 11 Jan 2018 02:56:24: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:56:24: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:56:24: #1 total tags in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:56:24: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:56:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:56:25: 39000000 INFO @ Thu, 11 Jan 2018 02:56:25: #1 tags after filtering in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:56:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:56:25: #1 finished! INFO @ Thu, 11 Jan 2018 02:56:25: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:56:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:56:27: #2 number of paired peaks: 6 WARNING @ Thu, 11 Jan 2018 02:56:27: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:56:27: Process for pairing-model is terminated! cat: SRX3270984.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3270984.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:56:31: 40000000 INFO @ Thu, 11 Jan 2018 02:56:32: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:56:32: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:56:32: #1 total tags in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:56:32: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:56:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:56:33: #1 tags after filtering in treatment: 40136060 INFO @ Thu, 11 Jan 2018 02:56:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:56:33: #1 finished! INFO @ Thu, 11 Jan 2018 02:56:33: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:56:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:56:36: #2 number of paired peaks: 6 WARNING @ Thu, 11 Jan 2018 02:56:36: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:56:36: Process for pairing-model is terminated! cat: SRX3270984.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3270984.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3270984.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。