Job ID = 1295109 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,282,290 reads read : 34,282,290 reads written : 34,282,290 spots read : 40,789,239 reads read : 40,789,239 reads written : 40,789,239 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:44 75071529 reads; of these: 75071529 (100.00%) were unpaired; of these: 1552338 (2.07%) aligned 0 times 26230340 (34.94%) aligned exactly 1 time 47288851 (62.99%) aligned >1 times 97.93% overall alignment rate Time searching: 00:40:44 Overall time: 00:40:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 25316322 / 73519191 = 0.3443 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:32:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:15: 1000000 INFO @ Mon, 03 Jun 2019 13:32:16: 1000000 INFO @ Mon, 03 Jun 2019 13:32:16: 1000000 INFO @ Mon, 03 Jun 2019 13:32:23: 2000000 INFO @ Mon, 03 Jun 2019 13:32:25: 2000000 INFO @ Mon, 03 Jun 2019 13:32:26: 2000000 INFO @ Mon, 03 Jun 2019 13:32:31: 3000000 INFO @ Mon, 03 Jun 2019 13:32:35: 3000000 INFO @ Mon, 03 Jun 2019 13:32:35: 3000000 INFO @ Mon, 03 Jun 2019 13:32:39: 4000000 INFO @ Mon, 03 Jun 2019 13:32:44: 4000000 INFO @ Mon, 03 Jun 2019 13:32:45: 4000000 INFO @ Mon, 03 Jun 2019 13:32:48: 5000000 INFO @ Mon, 03 Jun 2019 13:32:53: 5000000 INFO @ Mon, 03 Jun 2019 13:32:54: 5000000 INFO @ Mon, 03 Jun 2019 13:32:56: 6000000 INFO @ Mon, 03 Jun 2019 13:33:03: 6000000 INFO @ Mon, 03 Jun 2019 13:33:03: 6000000 INFO @ Mon, 03 Jun 2019 13:33:04: 7000000 INFO @ Mon, 03 Jun 2019 13:33:12: 7000000 INFO @ Mon, 03 Jun 2019 13:33:12: 8000000 INFO @ Mon, 03 Jun 2019 13:33:13: 7000000 INFO @ Mon, 03 Jun 2019 13:33:20: 9000000 INFO @ Mon, 03 Jun 2019 13:33:21: 8000000 INFO @ Mon, 03 Jun 2019 13:33:22: 8000000 INFO @ Mon, 03 Jun 2019 13:33:28: 10000000 INFO @ Mon, 03 Jun 2019 13:33:30: 9000000 INFO @ Mon, 03 Jun 2019 13:33:31: 9000000 INFO @ Mon, 03 Jun 2019 13:33:37: 11000000 INFO @ Mon, 03 Jun 2019 13:33:40: 10000000 INFO @ Mon, 03 Jun 2019 13:33:41: 10000000 INFO @ Mon, 03 Jun 2019 13:33:46: 12000000 INFO @ Mon, 03 Jun 2019 13:33:49: 11000000 INFO @ Mon, 03 Jun 2019 13:33:50: 11000000 INFO @ Mon, 03 Jun 2019 13:33:54: 13000000 INFO @ Mon, 03 Jun 2019 13:33:59: 12000000 INFO @ Mon, 03 Jun 2019 13:33:59: 12000000 INFO @ Mon, 03 Jun 2019 13:34:03: 14000000 INFO @ Mon, 03 Jun 2019 13:34:08: 13000000 INFO @ Mon, 03 Jun 2019 13:34:09: 13000000 INFO @ Mon, 03 Jun 2019 13:34:11: 15000000 INFO @ Mon, 03 Jun 2019 13:34:17: 14000000 INFO @ Mon, 03 Jun 2019 13:34:18: 14000000 INFO @ Mon, 03 Jun 2019 13:34:21: 16000000 INFO @ Mon, 03 Jun 2019 13:34:26: 15000000 INFO @ Mon, 03 Jun 2019 13:34:28: 15000000 INFO @ Mon, 03 Jun 2019 13:34:31: 17000000 INFO @ Mon, 03 Jun 2019 13:34:35: 16000000 INFO @ Mon, 03 Jun 2019 13:34:36: 16000000 INFO @ Mon, 03 Jun 2019 13:34:39: 18000000 INFO @ Mon, 03 Jun 2019 13:34:45: 17000000 INFO @ Mon, 03 Jun 2019 13:34:46: 17000000 INFO @ Mon, 03 Jun 2019 13:34:47: 19000000 INFO @ Mon, 03 Jun 2019 13:34:54: 18000000 INFO @ Mon, 03 Jun 2019 13:34:55: 18000000 INFO @ Mon, 03 Jun 2019 13:34:56: 20000000 INFO @ Mon, 03 Jun 2019 13:35:03: 19000000 INFO @ Mon, 03 Jun 2019 13:35:04: 21000000 INFO @ Mon, 03 Jun 2019 13:35:04: 19000000 INFO @ Mon, 03 Jun 2019 13:35:12: 20000000 INFO @ Mon, 03 Jun 2019 13:35:12: 22000000 INFO @ Mon, 03 Jun 2019 13:35:13: 20000000 INFO @ Mon, 03 Jun 2019 13:35:21: 23000000 INFO @ Mon, 03 Jun 2019 13:35:22: 21000000 INFO @ Mon, 03 Jun 2019 13:35:23: 21000000 INFO @ Mon, 03 Jun 2019 13:35:29: 24000000 INFO @ Mon, 03 Jun 2019 13:35:32: 22000000 INFO @ Mon, 03 Jun 2019 13:35:32: 22000000 INFO @ Mon, 03 Jun 2019 13:35:37: 25000000 INFO @ Mon, 03 Jun 2019 13:35:41: 23000000 INFO @ Mon, 03 Jun 2019 13:35:41: 23000000 INFO @ Mon, 03 Jun 2019 13:35:46: 26000000 INFO @ Mon, 03 Jun 2019 13:35:50: 24000000 INFO @ Mon, 03 Jun 2019 13:35:50: 24000000 INFO @ Mon, 03 Jun 2019 13:35:54: 27000000 INFO @ Mon, 03 Jun 2019 13:35:59: 25000000 INFO @ Mon, 03 Jun 2019 13:36:00: 25000000 INFO @ Mon, 03 Jun 2019 13:36:02: 28000000 INFO @ Mon, 03 Jun 2019 13:36:09: 26000000 INFO @ Mon, 03 Jun 2019 13:36:09: 26000000 INFO @ Mon, 03 Jun 2019 13:36:11: 29000000 INFO @ Mon, 03 Jun 2019 13:36:18: 27000000 INFO @ Mon, 03 Jun 2019 13:36:19: 27000000 INFO @ Mon, 03 Jun 2019 13:36:19: 30000000 INFO @ Mon, 03 Jun 2019 13:36:27: 28000000 INFO @ Mon, 03 Jun 2019 13:36:28: 28000000 INFO @ Mon, 03 Jun 2019 13:36:28: 31000000 INFO @ Mon, 03 Jun 2019 13:36:36: 32000000 INFO @ Mon, 03 Jun 2019 13:36:37: 29000000 INFO @ Mon, 03 Jun 2019 13:36:37: 29000000 INFO @ Mon, 03 Jun 2019 13:36:44: 33000000 INFO @ Mon, 03 Jun 2019 13:36:46: 30000000 INFO @ Mon, 03 Jun 2019 13:36:47: 30000000 INFO @ Mon, 03 Jun 2019 13:36:52: 34000000 INFO @ Mon, 03 Jun 2019 13:36:56: 31000000 INFO @ Mon, 03 Jun 2019 13:36:56: 31000000 INFO @ Mon, 03 Jun 2019 13:37:00: 35000000 INFO @ Mon, 03 Jun 2019 13:37:05: 32000000 INFO @ Mon, 03 Jun 2019 13:37:06: 32000000 INFO @ Mon, 03 Jun 2019 13:37:09: 36000000 INFO @ Mon, 03 Jun 2019 13:37:14: 33000000 INFO @ Mon, 03 Jun 2019 13:37:15: 33000000 INFO @ Mon, 03 Jun 2019 13:37:17: 37000000 INFO @ Mon, 03 Jun 2019 13:37:23: 34000000 INFO @ Mon, 03 Jun 2019 13:37:24: 34000000 INFO @ Mon, 03 Jun 2019 13:37:25: 38000000 INFO @ Mon, 03 Jun 2019 13:37:32: 35000000 INFO @ Mon, 03 Jun 2019 13:37:33: 39000000 INFO @ Mon, 03 Jun 2019 13:37:33: 35000000 INFO @ Mon, 03 Jun 2019 13:37:41: 36000000 INFO @ Mon, 03 Jun 2019 13:37:41: 40000000 INFO @ Mon, 03 Jun 2019 13:37:43: 36000000 INFO @ Mon, 03 Jun 2019 13:37:49: 41000000 INFO @ Mon, 03 Jun 2019 13:37:50: 37000000 INFO @ Mon, 03 Jun 2019 13:37:52: 37000000 INFO @ Mon, 03 Jun 2019 13:37:57: 42000000 INFO @ Mon, 03 Jun 2019 13:37:59: 38000000 INFO @ Mon, 03 Jun 2019 13:38:02: 38000000 INFO @ Mon, 03 Jun 2019 13:38:06: 43000000 INFO @ Mon, 03 Jun 2019 13:38:09: 39000000 INFO @ Mon, 03 Jun 2019 13:38:12: 39000000 INFO @ Mon, 03 Jun 2019 13:38:14: 44000000 INFO @ Mon, 03 Jun 2019 13:38:18: 40000000 INFO @ Mon, 03 Jun 2019 13:38:21: 40000000 INFO @ Mon, 03 Jun 2019 13:38:23: 45000000 INFO @ Mon, 03 Jun 2019 13:38:27: 41000000 INFO @ Mon, 03 Jun 2019 13:38:30: 41000000 INFO @ Mon, 03 Jun 2019 13:38:32: 46000000 INFO @ Mon, 03 Jun 2019 13:38:36: 42000000 INFO @ Mon, 03 Jun 2019 13:38:40: 42000000 INFO @ Mon, 03 Jun 2019 13:38:40: 47000000 INFO @ Mon, 03 Jun 2019 13:38:45: 43000000 INFO @ Mon, 03 Jun 2019 13:38:49: 43000000 INFO @ Mon, 03 Jun 2019 13:38:50: 48000000 INFO @ Mon, 03 Jun 2019 13:38:53: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:38:53: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:38:53: #1 total tags in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:38:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:38:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:38:54: #1 tags after filtering in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:38:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:38:54: #1 finished! INFO @ Mon, 03 Jun 2019 13:38:54: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:38:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:38:55: 44000000 INFO @ Mon, 03 Jun 2019 13:38:57: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:38:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:38:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:38:58: 44000000 INFO @ Mon, 03 Jun 2019 13:39:04: 45000000 INFO @ Mon, 03 Jun 2019 13:39:08: 45000000 INFO @ Mon, 03 Jun 2019 13:39:13: 46000000 INFO @ Mon, 03 Jun 2019 13:39:17: 46000000 INFO @ Mon, 03 Jun 2019 13:39:23: 47000000 INFO @ Mon, 03 Jun 2019 13:39:28: 47000000 INFO @ Mon, 03 Jun 2019 13:39:33: 48000000 INFO @ Mon, 03 Jun 2019 13:39:35: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:39:35: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:39:35: #1 total tags in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:39:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:39:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:39:36: #1 tags after filtering in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:39:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:39:36: #1 finished! INFO @ Mon, 03 Jun 2019 13:39:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:39:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:39:38: 48000000 INFO @ Mon, 03 Jun 2019 13:39:40: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:39:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:39:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:39:40: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:39:40: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:39:40: #1 total tags in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:39:40: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:39:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:39:41: #1 tags after filtering in treatment: 48202869 INFO @ Mon, 03 Jun 2019 13:39:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:39:41: #1 finished! INFO @ Mon, 03 Jun 2019 13:39:41: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:39:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:39:45: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:39:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:39:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318809/SRX318809.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。