Job ID = 10175278 sra ファイルのダウンロード中... Completed: 459893K bytes transferred in 5 seconds (661614K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 27474247 spots for /home/okishinya/chipatlas/results/dm3/SRX3159477/SRR6004207.sra Written 27474247 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:37 27474247 reads; of these: 27474247 (100.00%) were unpaired; of these: 760447 (2.77%) aligned 0 times 18261177 (66.47%) aligned exactly 1 time 8452623 (30.77%) aligned >1 times 97.23% overall alignment rate Time searching: 00:11:37 Overall time: 00:11:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2926380 / 26713800 = 0.1095 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Nov 2017 12:51:02: # Command line: callpeak -t SRX3159477.bam -f BAM -g dm -n SRX3159477.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3159477.10 # format = BAM # ChIP-seq file = ['SRX3159477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:51:02: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:51:02: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:51:02: # Command line: callpeak -t SRX3159477.bam -f BAM -g dm -n SRX3159477.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3159477.20 # format = BAM # ChIP-seq file = ['SRX3159477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:51:02: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:51:02: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:51:02: # Command line: callpeak -t SRX3159477.bam -f BAM -g dm -n SRX3159477.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3159477.05 # format = BAM # ChIP-seq file = ['SRX3159477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:51:02: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:51:02: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:51:11: 1000000 INFO @ Mon, 06 Nov 2017 12:51:11: 1000000 INFO @ Mon, 06 Nov 2017 12:51:11: 1000000 INFO @ Mon, 06 Nov 2017 12:51:19: 2000000 INFO @ Mon, 06 Nov 2017 12:51:19: 2000000 INFO @ Mon, 06 Nov 2017 12:51:19: 2000000 INFO @ Mon, 06 Nov 2017 12:51:27: 3000000 INFO @ Mon, 06 Nov 2017 12:51:27: 3000000 INFO @ Mon, 06 Nov 2017 12:51:27: 3000000 INFO @ Mon, 06 Nov 2017 12:51:35: 4000000 INFO @ Mon, 06 Nov 2017 12:51:35: 4000000 INFO @ Mon, 06 Nov 2017 12:51:35: 4000000 INFO @ Mon, 06 Nov 2017 12:51:43: 5000000 INFO @ Mon, 06 Nov 2017 12:51:43: 5000000 INFO @ Mon, 06 Nov 2017 12:51:43: 5000000 INFO @ Mon, 06 Nov 2017 12:51:51: 6000000 INFO @ Mon, 06 Nov 2017 12:51:51: 6000000 INFO @ Mon, 06 Nov 2017 12:51:51: 6000000 INFO @ Mon, 06 Nov 2017 12:51:59: 7000000 INFO @ Mon, 06 Nov 2017 12:51:59: 7000000 INFO @ Mon, 06 Nov 2017 12:51:59: 7000000 INFO @ Mon, 06 Nov 2017 12:52:07: 8000000 INFO @ Mon, 06 Nov 2017 12:52:07: 8000000 INFO @ Mon, 06 Nov 2017 12:52:07: 8000000 INFO @ Mon, 06 Nov 2017 12:52:15: 9000000 INFO @ Mon, 06 Nov 2017 12:52:15: 9000000 INFO @ Mon, 06 Nov 2017 12:52:15: 9000000 INFO @ Mon, 06 Nov 2017 12:52:23: 10000000 INFO @ Mon, 06 Nov 2017 12:52:23: 10000000 INFO @ Mon, 06 Nov 2017 12:52:23: 10000000 INFO @ Mon, 06 Nov 2017 12:52:31: 11000000 INFO @ Mon, 06 Nov 2017 12:52:31: 11000000 INFO @ Mon, 06 Nov 2017 12:52:31: 11000000 INFO @ Mon, 06 Nov 2017 12:52:40: 12000000 INFO @ Mon, 06 Nov 2017 12:52:40: 12000000 INFO @ Mon, 06 Nov 2017 12:52:40: 12000000 INFO @ Mon, 06 Nov 2017 12:52:47: 13000000 INFO @ Mon, 06 Nov 2017 12:52:47: 13000000 INFO @ Mon, 06 Nov 2017 12:52:47: 13000000 INFO @ Mon, 06 Nov 2017 12:52:55: 14000000 INFO @ Mon, 06 Nov 2017 12:52:55: 14000000 INFO @ Mon, 06 Nov 2017 12:52:55: 14000000 INFO @ Mon, 06 Nov 2017 12:53:04: 15000000 INFO @ Mon, 06 Nov 2017 12:53:04: 15000000 INFO @ Mon, 06 Nov 2017 12:53:04: 15000000 INFO @ Mon, 06 Nov 2017 12:53:11: 16000000 INFO @ Mon, 06 Nov 2017 12:53:11: 16000000 INFO @ Mon, 06 Nov 2017 12:53:11: 16000000 INFO @ Mon, 06 Nov 2017 12:53:19: 17000000 INFO @ Mon, 06 Nov 2017 12:53:19: 17000000 INFO @ Mon, 06 Nov 2017 12:53:19: 17000000 INFO @ Mon, 06 Nov 2017 12:53:27: 18000000 INFO @ Mon, 06 Nov 2017 12:53:27: 18000000 INFO @ Mon, 06 Nov 2017 12:53:27: 18000000 INFO @ Mon, 06 Nov 2017 12:53:35: 19000000 INFO @ Mon, 06 Nov 2017 12:53:35: 19000000 INFO @ Mon, 06 Nov 2017 12:53:35: 19000000 INFO @ Mon, 06 Nov 2017 12:53:43: 20000000 INFO @ Mon, 06 Nov 2017 12:53:43: 20000000 INFO @ Mon, 06 Nov 2017 12:53:43: 20000000 INFO @ Mon, 06 Nov 2017 12:53:51: 21000000 INFO @ Mon, 06 Nov 2017 12:53:51: 21000000 INFO @ Mon, 06 Nov 2017 12:53:51: 21000000 INFO @ Mon, 06 Nov 2017 12:53:59: 22000000 INFO @ Mon, 06 Nov 2017 12:53:59: 22000000 INFO @ Mon, 06 Nov 2017 12:53:59: 22000000 INFO @ Mon, 06 Nov 2017 12:54:07: 23000000 INFO @ Mon, 06 Nov 2017 12:54:07: 23000000 INFO @ Mon, 06 Nov 2017 12:54:07: 23000000 INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:54:14: #1 total tags in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 total tags in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:54:14: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:54:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:54:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:54:14: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:54:14: #1 total tags in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:54:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:54:14: #1 tags after filtering in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:54:14: #1 tags after filtering in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 finished! INFO @ Mon, 06 Nov 2017 12:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:54:14: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:54:14: #1 finished! INFO @ Mon, 06 Nov 2017 12:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:54:14: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:54:14: #1 tags after filtering in treatment: 23787420 INFO @ Mon, 06 Nov 2017 12:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:54:14: #1 finished! INFO @ Mon, 06 Nov 2017 12:54:14: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:54:16: #2 number of paired peaks: 63 WARNING @ Mon, 06 Nov 2017 12:54:16: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:54:16: Process for pairing-model is terminated! INFO @ Mon, 06 Nov 2017 12:54:16: #2 number of paired peaks: 63 WARNING @ Mon, 06 Nov 2017 12:54:16: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:54:16: Process for pairing-model is terminated! INFO @ Mon, 06 Nov 2017 12:54:16: #2 number of paired peaks: 63 WARNING @ Mon, 06 Nov 2017 12:54:16: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:54:16: Process for pairing-model is terminated! cat: SRX3159477.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3159477.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3159477.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3159477.20_model.r'rm: cannot remove `SRX3159477.05_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3159477.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159477.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。