Job ID = 10175263 sra ファイルのダウンロード中... Completed: 944133K bytes transferred in 19 seconds (394771K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 51859093 spots for /home/okishinya/chipatlas/results/dm3/SRX3159467/SRR6004197.sra Written 51859093 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:24:33 51859093 reads; of these: 51859093 (100.00%) were unpaired; of these: 1057229 (2.04%) aligned 0 times 34488617 (66.50%) aligned exactly 1 time 16313247 (31.46%) aligned >1 times 97.96% overall alignment rate Time searching: 00:24:35 Overall time: 00:24:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 8814334 / 50801864 = 0.1735 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Nov 2017 12:48:58: # Command line: callpeak -t SRX3159467.bam -f BAM -g dm -n SRX3159467.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3159467.20 # format = BAM # ChIP-seq file = ['SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:48:58: # Command line: callpeak -t SRX3159467.bam -f BAM -g dm -n SRX3159467.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3159467.10 # format = BAM # ChIP-seq file = ['SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:48:58: # Command line: callpeak -t SRX3159467.bam -f BAM -g dm -n SRX3159467.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3159467.05 # format = BAM # ChIP-seq file = ['SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:48:58: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:48:58: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:48:58: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:48:58: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:48:58: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:48:58: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:49:04: 1000000 INFO @ Mon, 06 Nov 2017 12:49:04: 1000000 INFO @ Mon, 06 Nov 2017 12:49:04: 1000000 INFO @ Mon, 06 Nov 2017 12:49:11: 2000000 INFO @ Mon, 06 Nov 2017 12:49:11: 2000000 INFO @ Mon, 06 Nov 2017 12:49:11: 2000000 INFO @ Mon, 06 Nov 2017 12:49:18: 3000000 INFO @ Mon, 06 Nov 2017 12:49:18: 3000000 INFO @ Mon, 06 Nov 2017 12:49:18: 3000000 INFO @ Mon, 06 Nov 2017 12:49:24: 4000000 INFO @ Mon, 06 Nov 2017 12:49:24: 4000000 INFO @ Mon, 06 Nov 2017 12:49:25: 4000000 INFO @ Mon, 06 Nov 2017 12:49:31: 5000000 INFO @ Mon, 06 Nov 2017 12:49:31: 5000000 INFO @ Mon, 06 Nov 2017 12:49:31: 5000000 INFO @ Mon, 06 Nov 2017 12:49:37: 6000000 INFO @ Mon, 06 Nov 2017 12:49:37: 6000000 INFO @ Mon, 06 Nov 2017 12:49:38: 6000000 INFO @ Mon, 06 Nov 2017 12:49:44: 7000000 INFO @ Mon, 06 Nov 2017 12:49:44: 7000000 INFO @ Mon, 06 Nov 2017 12:49:45: 7000000 INFO @ Mon, 06 Nov 2017 12:49:50: 8000000 INFO @ Mon, 06 Nov 2017 12:49:51: 8000000 INFO @ Mon, 06 Nov 2017 12:49:52: 8000000 INFO @ Mon, 06 Nov 2017 12:49:57: 9000000 INFO @ Mon, 06 Nov 2017 12:49:58: 9000000 INFO @ Mon, 06 Nov 2017 12:49:58: 9000000 INFO @ Mon, 06 Nov 2017 12:50:04: 10000000 INFO @ Mon, 06 Nov 2017 12:50:05: 10000000 INFO @ Mon, 06 Nov 2017 12:50:05: 10000000 INFO @ Mon, 06 Nov 2017 12:50:10: 11000000 INFO @ Mon, 06 Nov 2017 12:50:12: 11000000 INFO @ Mon, 06 Nov 2017 12:50:12: 11000000 INFO @ Mon, 06 Nov 2017 12:50:17: 12000000 INFO @ Mon, 06 Nov 2017 12:50:19: 12000000 INFO @ Mon, 06 Nov 2017 12:50:19: 12000000 INFO @ Mon, 06 Nov 2017 12:50:24: 13000000 INFO @ Mon, 06 Nov 2017 12:50:25: 13000000 INFO @ Mon, 06 Nov 2017 12:50:26: 13000000 INFO @ Mon, 06 Nov 2017 12:50:31: 14000000 INFO @ Mon, 06 Nov 2017 12:50:32: 14000000 INFO @ Mon, 06 Nov 2017 12:50:33: 14000000 INFO @ Mon, 06 Nov 2017 12:50:37: 15000000 INFO @ Mon, 06 Nov 2017 12:50:38: 15000000 INFO @ Mon, 06 Nov 2017 12:50:40: 15000000 INFO @ Mon, 06 Nov 2017 12:50:44: 16000000 INFO @ Mon, 06 Nov 2017 12:50:45: 16000000 INFO @ Mon, 06 Nov 2017 12:50:48: 16000000 INFO @ Mon, 06 Nov 2017 12:50:51: 17000000 INFO @ Mon, 06 Nov 2017 12:50:52: 17000000 INFO @ Mon, 06 Nov 2017 12:50:55: 17000000 INFO @ Mon, 06 Nov 2017 12:50:58: 18000000 INFO @ Mon, 06 Nov 2017 12:50:58: 18000000 INFO @ Mon, 06 Nov 2017 12:51:02: 18000000 INFO @ Mon, 06 Nov 2017 12:51:05: 19000000 INFO @ Mon, 06 Nov 2017 12:51:05: 19000000 INFO @ Mon, 06 Nov 2017 12:51:09: 19000000 INFO @ Mon, 06 Nov 2017 12:51:11: 20000000 INFO @ Mon, 06 Nov 2017 12:51:12: 20000000 INFO @ Mon, 06 Nov 2017 12:51:16: 20000000 INFO @ Mon, 06 Nov 2017 12:51:18: 21000000 INFO @ Mon, 06 Nov 2017 12:51:19: 21000000 INFO @ Mon, 06 Nov 2017 12:51:24: 21000000 INFO @ Mon, 06 Nov 2017 12:51:24: 22000000 INFO @ Mon, 06 Nov 2017 12:51:26: 22000000 INFO @ Mon, 06 Nov 2017 12:51:31: 22000000 INFO @ Mon, 06 Nov 2017 12:51:31: 23000000 INFO @ Mon, 06 Nov 2017 12:51:33: 23000000 INFO @ Mon, 06 Nov 2017 12:51:38: 24000000 INFO @ Mon, 06 Nov 2017 12:51:38: 23000000 INFO @ Mon, 06 Nov 2017 12:51:39: 24000000 INFO @ Mon, 06 Nov 2017 12:51:44: 25000000 INFO @ Mon, 06 Nov 2017 12:51:45: 24000000 INFO @ Mon, 06 Nov 2017 12:51:46: 25000000 INFO @ Mon, 06 Nov 2017 12:51:51: 26000000 INFO @ Mon, 06 Nov 2017 12:51:52: 25000000 INFO @ Mon, 06 Nov 2017 12:51:53: 26000000 INFO @ Mon, 06 Nov 2017 12:51:57: 27000000 INFO @ Mon, 06 Nov 2017 12:51:59: 26000000 INFO @ Mon, 06 Nov 2017 12:52:00: 27000000 INFO @ Mon, 06 Nov 2017 12:52:04: 28000000 INFO @ Mon, 06 Nov 2017 12:52:06: 27000000 INFO @ Mon, 06 Nov 2017 12:52:07: 28000000 INFO @ Mon, 06 Nov 2017 12:52:10: 29000000 INFO @ Mon, 06 Nov 2017 12:52:13: 28000000 INFO @ Mon, 06 Nov 2017 12:52:14: 29000000 INFO @ Mon, 06 Nov 2017 12:52:17: 30000000 INFO @ Mon, 06 Nov 2017 12:52:21: 29000000 INFO @ Mon, 06 Nov 2017 12:52:21: 30000000 INFO @ Mon, 06 Nov 2017 12:52:24: 31000000 INFO @ Mon, 06 Nov 2017 12:52:28: 31000000 INFO @ Mon, 06 Nov 2017 12:52:28: 30000000 INFO @ Mon, 06 Nov 2017 12:52:30: 32000000 INFO @ Mon, 06 Nov 2017 12:52:35: 32000000 INFO @ Mon, 06 Nov 2017 12:52:35: 31000000 INFO @ Mon, 06 Nov 2017 12:52:37: 33000000 INFO @ Mon, 06 Nov 2017 12:52:42: 33000000 INFO @ Mon, 06 Nov 2017 12:52:42: 32000000 INFO @ Mon, 06 Nov 2017 12:52:43: 34000000 INFO @ Mon, 06 Nov 2017 12:52:49: 34000000 INFO @ Mon, 06 Nov 2017 12:52:50: 35000000 INFO @ Mon, 06 Nov 2017 12:52:50: 33000000 INFO @ Mon, 06 Nov 2017 12:52:56: 35000000 INFO @ Mon, 06 Nov 2017 12:52:56: 36000000 INFO @ Mon, 06 Nov 2017 12:52:57: 34000000 INFO @ Mon, 06 Nov 2017 12:53:03: 37000000 INFO @ Mon, 06 Nov 2017 12:53:03: 36000000 INFO @ Mon, 06 Nov 2017 12:53:05: 35000000 INFO @ Mon, 06 Nov 2017 12:53:09: 38000000 INFO @ Mon, 06 Nov 2017 12:53:10: 37000000 INFO @ Mon, 06 Nov 2017 12:53:12: 36000000 INFO @ Mon, 06 Nov 2017 12:53:16: 39000000 INFO @ Mon, 06 Nov 2017 12:53:17: 38000000 INFO @ Mon, 06 Nov 2017 12:53:19: 37000000 INFO @ Mon, 06 Nov 2017 12:53:22: 40000000 INFO @ Mon, 06 Nov 2017 12:53:24: 39000000 INFO @ Mon, 06 Nov 2017 12:53:26: 38000000 INFO @ Mon, 06 Nov 2017 12:53:29: 41000000 INFO @ Mon, 06 Nov 2017 12:53:31: 40000000 INFO @ Mon, 06 Nov 2017 12:53:34: 39000000 INFO @ Mon, 06 Nov 2017 12:53:35: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:53:35: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:53:35: #1 total tags in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:35: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:53:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:53:36: #1 tags after filtering in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:53:36: #1 finished! INFO @ Mon, 06 Nov 2017 12:53:36: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:53:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:53:38: 41000000 INFO @ Mon, 06 Nov 2017 12:53:39: #2 number of paired peaks: 1 WARNING @ Mon, 06 Nov 2017 12:53:39: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:53:39: Process for pairing-model is terminated! cat: SRX3159467.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3159467.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Nov 2017 12:53:41: 40000000 INFO @ Mon, 06 Nov 2017 12:53:45: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:53:45: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:53:45: #1 total tags in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:45: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:53:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:53:46: #1 tags after filtering in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:53:46: #1 finished! INFO @ Mon, 06 Nov 2017 12:53:46: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:53:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:53:48: 41000000 INFO @ Mon, 06 Nov 2017 12:53:48: #2 number of paired peaks: 1 WARNING @ Mon, 06 Nov 2017 12:53:48: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:53:48: Process for pairing-model is terminated! cat: SRX3159467.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3159467.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Nov 2017 12:53:54: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:53:54: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:53:54: #1 total tags in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:54: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:53:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:53:55: #1 tags after filtering in treatment: 41987530 INFO @ Mon, 06 Nov 2017 12:53:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:53:55: #1 finished! INFO @ Mon, 06 Nov 2017 12:53:55: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:53:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:53:58: #2 number of paired peaks: 1 WARNING @ Mon, 06 Nov 2017 12:53:58: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:53:58: Process for pairing-model is terminated! cat: SRX3159467.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3159467.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3159467.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。