Job ID = 12264979 SRX = SRX3088424 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5516 spots for SRR5928102/SRR5928102.sra Written 5516 spots for SRR5928102/SRR5928102.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265137 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 5516 reads; of these: 5516 (100.00%) were unpaired; of these: 3485 (63.18%) aligned 0 times 1591 (28.84%) aligned exactly 1 time 440 (7.98%) aligned >1 times 36.82% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 652 / 2031 = 0.3210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:03:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:03:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:03:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:03:32: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:03:32: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:03:32: #1 total tags in treatment: 1379 INFO @ Sat, 03 Apr 2021 06:03:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:03:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:03:32: #1 tags after filtering in treatment: 1377 INFO @ Sat, 03 Apr 2021 06:03:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:03:32: #1 finished! INFO @ Sat, 03 Apr 2021 06:03:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:03:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:03:32: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:03:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:03:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:04:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:04:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:04:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:04:02: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:04:02: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:04:02: #1 total tags in treatment: 1379 INFO @ Sat, 03 Apr 2021 06:04:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:04:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:04:02: #1 tags after filtering in treatment: 1377 INFO @ Sat, 03 Apr 2021 06:04:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:04:02: #1 finished! INFO @ Sat, 03 Apr 2021 06:04:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:04:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:04:02: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:04:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:04:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:04:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:04:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:04:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:04:32: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:04:32: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:04:32: #1 total tags in treatment: 1379 INFO @ Sat, 03 Apr 2021 06:04:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:04:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:04:32: #1 tags after filtering in treatment: 1377 INFO @ Sat, 03 Apr 2021 06:04:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:04:32: #1 finished! INFO @ Sat, 03 Apr 2021 06:04:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:04:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:04:32: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:04:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:04:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088424/SRX3088424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling