Job ID = 12264977 SRX = SRX3088422 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6719 spots for SRR5928100/SRR5928100.sra Written 6719 spots for SRR5928100/SRR5928100.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265132 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 6719 reads; of these: 6719 (100.00%) were unpaired; of these: 4029 (59.96%) aligned 0 times 2042 (30.39%) aligned exactly 1 time 648 (9.64%) aligned >1 times 40.04% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 759 / 2690 = 0.2822 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:03:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:03:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:03:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:03:15: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:03:15: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:03:15: #1 total tags in treatment: 1931 INFO @ Sat, 03 Apr 2021 06:03:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:03:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:03:15: #1 tags after filtering in treatment: 1930 INFO @ Sat, 03 Apr 2021 06:03:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:03:15: #1 finished! INFO @ Sat, 03 Apr 2021 06:03:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:03:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:03:15: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:03:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:03:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:03:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:03:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:03:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:03:45: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:03:45: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:03:45: #1 total tags in treatment: 1931 INFO @ Sat, 03 Apr 2021 06:03:45: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:03:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:03:45: #1 tags after filtering in treatment: 1930 INFO @ Sat, 03 Apr 2021 06:03:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:03:45: #1 finished! INFO @ Sat, 03 Apr 2021 06:03:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:03:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:03:45: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:03:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:03:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:04:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:04:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:04:15: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:04:15: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:04:15: #1 total tags in treatment: 1931 INFO @ Sat, 03 Apr 2021 06:04:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:04:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:04:15: #1 tags after filtering in treatment: 1930 INFO @ Sat, 03 Apr 2021 06:04:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:04:15: #1 finished! INFO @ Sat, 03 Apr 2021 06:04:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:04:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:04:15: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 06:04:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 06:04:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3088422/SRX3088422.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling