Job ID = 12264899 SRX = SRX3088350 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13844 spots for SRR5928028/SRR5928028.sra Written 13844 spots for SRR5928028/SRR5928028.sra fastq に変換しました。 bowtie でマッピング中... Your job 12264953 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 13844 reads; of these: 13844 (100.00%) were unpaired; of these: 6150 (44.42%) aligned 0 times 5991 (43.28%) aligned exactly 1 time 1703 (12.30%) aligned >1 times 55.58% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2087 / 7694 = 0.2713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:55:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:55:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:55:20: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 05:55:20: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 05:55:20: #1 total tags in treatment: 5607 INFO @ Sat, 03 Apr 2021 05:55:20: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:55:20: #1 tags after filtering in treatment: 5606 INFO @ Sat, 03 Apr 2021 05:55:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:55:20: #1 finished! INFO @ Sat, 03 Apr 2021 05:55:20: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:55:20: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 05:55:20: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 05:55:20: start model_add_line... INFO @ Sat, 03 Apr 2021 05:55:20: start X-correlation... INFO @ Sat, 03 Apr 2021 05:55:20: end of X-cor INFO @ Sat, 03 Apr 2021 05:55:20: #2 finished! INFO @ Sat, 03 Apr 2021 05:55:20: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 05:55:20: #2 alternative fragment length(s) may be 8,56,77,95,140,166,201,237,279,330,469,508,547 bps INFO @ Sat, 03 Apr 2021 05:55:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05_model.r WARNING @ Sat, 03 Apr 2021 05:55:20: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:55:20: #2 You may need to consider one of the other alternative d(s): 8,56,77,95,140,166,201,237,279,330,469,508,547 WARNING @ Sat, 03 Apr 2021 05:55:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:55:20: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:55:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 05:55:20: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:55:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05_peaks.xls INFO @ Sat, 03 Apr 2021 05:55:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:55:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.05_summits.bed INFO @ Sat, 03 Apr 2021 05:55:20: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:55:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:55:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:55:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:55:49: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 05:55:49: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 05:55:49: #1 total tags in treatment: 5607 INFO @ Sat, 03 Apr 2021 05:55:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:55:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:55:49: #1 tags after filtering in treatment: 5606 INFO @ Sat, 03 Apr 2021 05:55:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:55:49: #1 finished! INFO @ Sat, 03 Apr 2021 05:55:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:55:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:55:49: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 05:55:49: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 05:55:49: start model_add_line... INFO @ Sat, 03 Apr 2021 05:55:49: start X-correlation... INFO @ Sat, 03 Apr 2021 05:55:49: end of X-cor INFO @ Sat, 03 Apr 2021 05:55:49: #2 finished! INFO @ Sat, 03 Apr 2021 05:55:49: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 05:55:49: #2 alternative fragment length(s) may be 8,56,77,95,140,166,201,237,279,330,469,508,547 bps INFO @ Sat, 03 Apr 2021 05:55:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10_model.r WARNING @ Sat, 03 Apr 2021 05:55:49: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:55:49: #2 You may need to consider one of the other alternative d(s): 8,56,77,95,140,166,201,237,279,330,469,508,547 WARNING @ Sat, 03 Apr 2021 05:55:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:55:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:55:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 05:55:49: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:55:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10_peaks.xls INFO @ Sat, 03 Apr 2021 05:55:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:55:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.10_summits.bed INFO @ Sat, 03 Apr 2021 05:55:49: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 05:56:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:56:19: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:56:19: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 05:56:19: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 05:56:19: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 05:56:19: #1 total tags in treatment: 5607 INFO @ Sat, 03 Apr 2021 05:56:19: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 05:56:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 05:56:19: #1 tags after filtering in treatment: 5606 INFO @ Sat, 03 Apr 2021 05:56:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 05:56:19: #1 finished! INFO @ Sat, 03 Apr 2021 05:56:19: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 05:56:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 05:56:19: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 05:56:19: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 05:56:19: start model_add_line... INFO @ Sat, 03 Apr 2021 05:56:19: start X-correlation... INFO @ Sat, 03 Apr 2021 05:56:19: end of X-cor INFO @ Sat, 03 Apr 2021 05:56:19: #2 finished! INFO @ Sat, 03 Apr 2021 05:56:19: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 05:56:19: #2 alternative fragment length(s) may be 8,56,77,95,140,166,201,237,279,330,469,508,547 bps INFO @ Sat, 03 Apr 2021 05:56:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20_model.r WARNING @ Sat, 03 Apr 2021 05:56:19: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 05:56:19: #2 You may need to consider one of the other alternative d(s): 8,56,77,95,140,166,201,237,279,330,469,508,547 WARNING @ Sat, 03 Apr 2021 05:56:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 05:56:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 05:56:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 05:56:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 05:56:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20_peaks.xls INFO @ Sat, 03 Apr 2021 05:56:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 05:56:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088350/SRX3088350.20_summits.bed INFO @ Sat, 03 Apr 2021 05:56:19: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling