Job ID = 10480706 sra ファイルのダウンロード中... Completed: 234350K bytes transferred in 22 seconds (85759K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 8079238 spots for /home/okishinya/chipatlas/results/dm3/SRX3068977/SRR5907453.sra Written 8079238 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:04 8079238 reads; of these: 8079238 (100.00%) were unpaired; of these: 2460708 (30.46%) aligned 0 times 4912807 (60.81%) aligned exactly 1 time 705723 (8.74%) aligned >1 times 69.54% overall alignment rate Time searching: 00:02:04 Overall time: 00:02:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1683477 / 5618530 = 0.2996 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 16 Mar 2018 07:44:16: # Command line: callpeak -t SRX3068977.bam -f BAM -g dm -n SRX3068977.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3068977.05 # format = BAM # ChIP-seq file = ['SRX3068977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:44:16: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:44:16: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:44:16: # Command line: callpeak -t SRX3068977.bam -f BAM -g dm -n SRX3068977.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3068977.20 # format = BAM # ChIP-seq file = ['SRX3068977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:44:16: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:44:16: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:44:16: # Command line: callpeak -t SRX3068977.bam -f BAM -g dm -n SRX3068977.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3068977.10 # format = BAM # ChIP-seq file = ['SRX3068977.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:44:16: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:44:16: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:44:22: 1000000 INFO @ Fri, 16 Mar 2018 07:44:22: 1000000 INFO @ Fri, 16 Mar 2018 07:44:23: 1000000 INFO @ Fri, 16 Mar 2018 07:44:29: 2000000 INFO @ Fri, 16 Mar 2018 07:44:29: 2000000 INFO @ Fri, 16 Mar 2018 07:44:29: 2000000 INFO @ Fri, 16 Mar 2018 07:44:36: 3000000 INFO @ Fri, 16 Mar 2018 07:44:36: 3000000 INFO @ Fri, 16 Mar 2018 07:44:36: 3000000 INFO @ Fri, 16 Mar 2018 07:44:42: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:44:42: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:44:42: #1 total tags in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:42: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:44:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:44:42: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:44:42: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:44:42: #1 total tags in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:42: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:44:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:44:42: #1 tags after filtering in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:44:42: #1 finished! INFO @ Fri, 16 Mar 2018 07:44:42: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:44:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:44:42: #1 tags after filtering in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:44:42: #1 finished! INFO @ Fri, 16 Mar 2018 07:44:42: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:44:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:44:43: #2 number of paired peaks: 3281 INFO @ Fri, 16 Mar 2018 07:44:43: start model_add_line... INFO @ Fri, 16 Mar 2018 07:44:43: #2 number of paired peaks: 3281 INFO @ Fri, 16 Mar 2018 07:44:43: start model_add_line... INFO @ Fri, 16 Mar 2018 07:44:43: start X-correlation... INFO @ Fri, 16 Mar 2018 07:44:43: end of X-cor INFO @ Fri, 16 Mar 2018 07:44:43: #2 finished! INFO @ Fri, 16 Mar 2018 07:44:43: #2 predicted fragment length is 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2 alternative fragment length(s) may be 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2.2 Generate R script for model : SRX3068977.05_model.r INFO @ Fri, 16 Mar 2018 07:44:43: #3 Call peaks... INFO @ Fri, 16 Mar 2018 07:44:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Mar 2018 07:44:43: start X-correlation... INFO @ Fri, 16 Mar 2018 07:44:43: end of X-cor INFO @ Fri, 16 Mar 2018 07:44:43: #2 finished! INFO @ Fri, 16 Mar 2018 07:44:43: #2 predicted fragment length is 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2 alternative fragment length(s) may be 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2.2 Generate R script for model : SRX3068977.20_model.r INFO @ Fri, 16 Mar 2018 07:44:43: #3 Call peaks... INFO @ Fri, 16 Mar 2018 07:44:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Mar 2018 07:44:43: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:44:43: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:44:43: #1 total tags in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:43: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:44:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:44:43: #1 tags after filtering in treatment: 3935053 INFO @ Fri, 16 Mar 2018 07:44:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:44:43: #1 finished! INFO @ Fri, 16 Mar 2018 07:44:43: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:44:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:44:43: #2 number of paired peaks: 3281 INFO @ Fri, 16 Mar 2018 07:44:43: start model_add_line... INFO @ Fri, 16 Mar 2018 07:44:43: start X-correlation... INFO @ Fri, 16 Mar 2018 07:44:43: end of X-cor INFO @ Fri, 16 Mar 2018 07:44:43: #2 finished! INFO @ Fri, 16 Mar 2018 07:44:43: #2 predicted fragment length is 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2 alternative fragment length(s) may be 169 bps INFO @ Fri, 16 Mar 2018 07:44:43: #2.2 Generate R script for model : SRX3068977.10_model.r INFO @ Fri, 16 Mar 2018 07:44:43: #3 Call peaks... INFO @ Fri, 16 Mar 2018 07:44:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Mar 2018 07:44:53: #3 Call peaks for each chromosome... INFO @ Fri, 16 Mar 2018 07:44:53: #3 Call peaks for each chromosome... INFO @ Fri, 16 Mar 2018 07:44:54: #3 Call peaks for each chromosome... INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write output xls file... SRX3068977.20_peaks.xls INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write output xls file... SRX3068977.05_peaks.xls INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write peak in narrowPeak format file... SRX3068977.20_peaks.narrowPeak INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write summits bed file... SRX3068977.20_summits.bed INFO @ Fri, 16 Mar 2018 07:44:58: Done! INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write peak in narrowPeak format file... SRX3068977.05_peaks.narrowPeak pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (1917 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 16 Mar 2018 07:44:58: #4 Write summits bed file... SRX3068977.05_summits.bed INFO @ Fri, 16 Mar 2018 07:44:58: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (5671 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 16 Mar 2018 07:44:59: #4 Write output xls file... SRX3068977.10_peaks.xls INFO @ Fri, 16 Mar 2018 07:44:59: #4 Write peak in narrowPeak format file... SRX3068977.10_peaks.narrowPeak INFO @ Fri, 16 Mar 2018 07:45:00: #4 Write summits bed file... SRX3068977.10_summits.bed INFO @ Fri, 16 Mar 2018 07:45:00: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (3533 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。