Job ID = 1295011 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 51,671,313 reads read : 103,342,626 reads written : 103,342,626 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:16:54 51671313 reads; of these: 51671313 (100.00%) were paired; of these: 14542005 (28.14%) aligned concordantly 0 times 25450961 (49.26%) aligned concordantly exactly 1 time 11678347 (22.60%) aligned concordantly >1 times ---- 14542005 pairs aligned concordantly 0 times; of these: 185416 (1.28%) aligned discordantly 1 time ---- 14356589 pairs aligned 0 times concordantly or discordantly; of these: 28713178 mates make up the pairs; of these: 24675187 (85.94%) aligned 0 times 2663034 (9.27%) aligned exactly 1 time 1374957 (4.79%) aligned >1 times 76.12% overall alignment rate Time searching: 01:16:54 Overall time: 01:16:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4778169 / 37139169 = 0.1287 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:14:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:14:41: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:14:41: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:14:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:14:41: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:14:41: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:14:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:14:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:14:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:14:48: 1000000 INFO @ Mon, 03 Jun 2019 13:14:48: 1000000 INFO @ Mon, 03 Jun 2019 13:14:49: 1000000 INFO @ Mon, 03 Jun 2019 13:14:53: 2000000 INFO @ Mon, 03 Jun 2019 13:14:55: 2000000 INFO @ Mon, 03 Jun 2019 13:14:56: 2000000 INFO @ Mon, 03 Jun 2019 13:14:59: 3000000 INFO @ Mon, 03 Jun 2019 13:15:02: 3000000 INFO @ Mon, 03 Jun 2019 13:15:03: 3000000 INFO @ Mon, 03 Jun 2019 13:15:05: 4000000 INFO @ Mon, 03 Jun 2019 13:15:09: 4000000 INFO @ Mon, 03 Jun 2019 13:15:10: 4000000 INFO @ Mon, 03 Jun 2019 13:15:10: 5000000 INFO @ Mon, 03 Jun 2019 13:15:16: 5000000 INFO @ Mon, 03 Jun 2019 13:15:16: 6000000 INFO @ Mon, 03 Jun 2019 13:15:16: 5000000 INFO @ Mon, 03 Jun 2019 13:15:22: 6000000 INFO @ Mon, 03 Jun 2019 13:15:22: 7000000 INFO @ Mon, 03 Jun 2019 13:15:23: 6000000 INFO @ Mon, 03 Jun 2019 13:15:28: 8000000 INFO @ Mon, 03 Jun 2019 13:15:29: 7000000 INFO @ Mon, 03 Jun 2019 13:15:30: 7000000 INFO @ Mon, 03 Jun 2019 13:15:34: 9000000 INFO @ Mon, 03 Jun 2019 13:15:36: 8000000 INFO @ Mon, 03 Jun 2019 13:15:37: 8000000 INFO @ Mon, 03 Jun 2019 13:15:40: 10000000 INFO @ Mon, 03 Jun 2019 13:15:42: 9000000 INFO @ Mon, 03 Jun 2019 13:15:43: 9000000 INFO @ Mon, 03 Jun 2019 13:15:46: 11000000 INFO @ Mon, 03 Jun 2019 13:15:49: 10000000 INFO @ Mon, 03 Jun 2019 13:15:50: 10000000 INFO @ Mon, 03 Jun 2019 13:15:52: 12000000 INFO @ Mon, 03 Jun 2019 13:15:55: 11000000 INFO @ Mon, 03 Jun 2019 13:15:57: 11000000 INFO @ Mon, 03 Jun 2019 13:15:58: 13000000 INFO @ Mon, 03 Jun 2019 13:16:02: 12000000 INFO @ Mon, 03 Jun 2019 13:16:03: 12000000 INFO @ Mon, 03 Jun 2019 13:16:04: 14000000 INFO @ Mon, 03 Jun 2019 13:16:08: 13000000 INFO @ Mon, 03 Jun 2019 13:16:10: 13000000 INFO @ Mon, 03 Jun 2019 13:16:10: 15000000 INFO @ Mon, 03 Jun 2019 13:16:15: 14000000 INFO @ Mon, 03 Jun 2019 13:16:16: 14000000 INFO @ Mon, 03 Jun 2019 13:16:17: 16000000 INFO @ Mon, 03 Jun 2019 13:16:21: 15000000 INFO @ Mon, 03 Jun 2019 13:16:23: 15000000 INFO @ Mon, 03 Jun 2019 13:16:24: 17000000 INFO @ Mon, 03 Jun 2019 13:16:26: 16000000 INFO @ Mon, 03 Jun 2019 13:16:29: 16000000 INFO @ Mon, 03 Jun 2019 13:16:30: 18000000 INFO @ Mon, 03 Jun 2019 13:16:32: 17000000 INFO @ Mon, 03 Jun 2019 13:16:35: 17000000 INFO @ Mon, 03 Jun 2019 13:16:36: 19000000 INFO @ Mon, 03 Jun 2019 13:16:38: 18000000 INFO @ Mon, 03 Jun 2019 13:16:41: 18000000 INFO @ Mon, 03 Jun 2019 13:16:43: 20000000 INFO @ Mon, 03 Jun 2019 13:16:44: 19000000 INFO @ Mon, 03 Jun 2019 13:16:47: 19000000 INFO @ Mon, 03 Jun 2019 13:16:50: 21000000 INFO @ Mon, 03 Jun 2019 13:16:50: 20000000 INFO @ Mon, 03 Jun 2019 13:16:54: 20000000 INFO @ Mon, 03 Jun 2019 13:16:56: 21000000 INFO @ Mon, 03 Jun 2019 13:16:57: 22000000 INFO @ Mon, 03 Jun 2019 13:17:00: 21000000 INFO @ Mon, 03 Jun 2019 13:17:01: 22000000 INFO @ Mon, 03 Jun 2019 13:17:04: 23000000 INFO @ Mon, 03 Jun 2019 13:17:06: 22000000 INFO @ Mon, 03 Jun 2019 13:17:07: 23000000 INFO @ Mon, 03 Jun 2019 13:17:11: 24000000 INFO @ Mon, 03 Jun 2019 13:17:12: 23000000 INFO @ Mon, 03 Jun 2019 13:17:12: 24000000 INFO @ Mon, 03 Jun 2019 13:17:17: 25000000 INFO @ Mon, 03 Jun 2019 13:17:18: 25000000 INFO @ Mon, 03 Jun 2019 13:17:18: 24000000 INFO @ Mon, 03 Jun 2019 13:17:24: 26000000 INFO @ Mon, 03 Jun 2019 13:17:24: 25000000 INFO @ Mon, 03 Jun 2019 13:17:25: 26000000 INFO @ Mon, 03 Jun 2019 13:17:29: 27000000 INFO @ Mon, 03 Jun 2019 13:17:30: 26000000 INFO @ Mon, 03 Jun 2019 13:17:32: 27000000 INFO @ Mon, 03 Jun 2019 13:17:35: 28000000 INFO @ Mon, 03 Jun 2019 13:17:37: 27000000 INFO @ Mon, 03 Jun 2019 13:17:39: 28000000 INFO @ Mon, 03 Jun 2019 13:17:41: 29000000 INFO @ Mon, 03 Jun 2019 13:17:43: 28000000 INFO @ Mon, 03 Jun 2019 13:17:46: 29000000 INFO @ Mon, 03 Jun 2019 13:17:46: 30000000 INFO @ Mon, 03 Jun 2019 13:17:49: 29000000 INFO @ Mon, 03 Jun 2019 13:17:52: 31000000 INFO @ Mon, 03 Jun 2019 13:17:53: 30000000 INFO @ Mon, 03 Jun 2019 13:17:55: 30000000 INFO @ Mon, 03 Jun 2019 13:17:57: 32000000 INFO @ Mon, 03 Jun 2019 13:18:00: 31000000 INFO @ Mon, 03 Jun 2019 13:18:01: 31000000 INFO @ Mon, 03 Jun 2019 13:18:03: 33000000 INFO @ Mon, 03 Jun 2019 13:18:07: 32000000 INFO @ Mon, 03 Jun 2019 13:18:07: 32000000 INFO @ Mon, 03 Jun 2019 13:18:09: 34000000 INFO @ Mon, 03 Jun 2019 13:18:13: 33000000 INFO @ Mon, 03 Jun 2019 13:18:14: 33000000 INFO @ Mon, 03 Jun 2019 13:18:14: 35000000 INFO @ Mon, 03 Jun 2019 13:18:20: 34000000 INFO @ Mon, 03 Jun 2019 13:18:20: 36000000 INFO @ Mon, 03 Jun 2019 13:18:22: 34000000 INFO @ Mon, 03 Jun 2019 13:18:26: 37000000 INFO @ Mon, 03 Jun 2019 13:18:26: 35000000 INFO @ Mon, 03 Jun 2019 13:18:29: 35000000 INFO @ Mon, 03 Jun 2019 13:18:31: 38000000 INFO @ Mon, 03 Jun 2019 13:18:32: 36000000 INFO @ Mon, 03 Jun 2019 13:18:36: 36000000 INFO @ Mon, 03 Jun 2019 13:18:37: 39000000 INFO @ Mon, 03 Jun 2019 13:18:38: 37000000 INFO @ Mon, 03 Jun 2019 13:18:42: 40000000 INFO @ Mon, 03 Jun 2019 13:18:42: 37000000 INFO @ Mon, 03 Jun 2019 13:18:44: 38000000 INFO @ Mon, 03 Jun 2019 13:18:48: 41000000 INFO @ Mon, 03 Jun 2019 13:18:49: 38000000 INFO @ Mon, 03 Jun 2019 13:18:50: 39000000 INFO @ Mon, 03 Jun 2019 13:18:53: 42000000 INFO @ Mon, 03 Jun 2019 13:18:56: 40000000 INFO @ Mon, 03 Jun 2019 13:18:56: 39000000 INFO @ Mon, 03 Jun 2019 13:18:59: 43000000 INFO @ Mon, 03 Jun 2019 13:19:02: 41000000 INFO @ Mon, 03 Jun 2019 13:19:03: 40000000 INFO @ Mon, 03 Jun 2019 13:19:05: 44000000 INFO @ Mon, 03 Jun 2019 13:19:08: 42000000 INFO @ Mon, 03 Jun 2019 13:19:10: 41000000 INFO @ Mon, 03 Jun 2019 13:19:10: 45000000 INFO @ Mon, 03 Jun 2019 13:19:14: 43000000 INFO @ Mon, 03 Jun 2019 13:19:16: 46000000 INFO @ Mon, 03 Jun 2019 13:19:17: 42000000 INFO @ Mon, 03 Jun 2019 13:19:20: 44000000 INFO @ Mon, 03 Jun 2019 13:19:21: 47000000 INFO @ Mon, 03 Jun 2019 13:19:24: 43000000 INFO @ Mon, 03 Jun 2019 13:19:26: 45000000 INFO @ Mon, 03 Jun 2019 13:19:27: 48000000 INFO @ Mon, 03 Jun 2019 13:19:31: 44000000 INFO @ Mon, 03 Jun 2019 13:19:32: 49000000 INFO @ Mon, 03 Jun 2019 13:19:32: 46000000 INFO @ Mon, 03 Jun 2019 13:19:38: 45000000 INFO @ Mon, 03 Jun 2019 13:19:38: 50000000 INFO @ Mon, 03 Jun 2019 13:19:38: 47000000 INFO @ Mon, 03 Jun 2019 13:19:43: 51000000 INFO @ Mon, 03 Jun 2019 13:19:44: 48000000 INFO @ Mon, 03 Jun 2019 13:19:45: 46000000 INFO @ Mon, 03 Jun 2019 13:19:49: 52000000 INFO @ Mon, 03 Jun 2019 13:19:50: 49000000 INFO @ Mon, 03 Jun 2019 13:19:51: 47000000 INFO @ Mon, 03 Jun 2019 13:19:54: 53000000 INFO @ Mon, 03 Jun 2019 13:19:56: 50000000 INFO @ Mon, 03 Jun 2019 13:19:58: 48000000 INFO @ Mon, 03 Jun 2019 13:19:59: 54000000 INFO @ Mon, 03 Jun 2019 13:20:02: 51000000 INFO @ Mon, 03 Jun 2019 13:20:05: 49000000 INFO @ Mon, 03 Jun 2019 13:20:05: 55000000 INFO @ Mon, 03 Jun 2019 13:20:08: 52000000 INFO @ Mon, 03 Jun 2019 13:20:11: 56000000 INFO @ Mon, 03 Jun 2019 13:20:11: 50000000 INFO @ Mon, 03 Jun 2019 13:20:13: 53000000 INFO @ Mon, 03 Jun 2019 13:20:16: 57000000 INFO @ Mon, 03 Jun 2019 13:20:18: 51000000 INFO @ Mon, 03 Jun 2019 13:20:19: 54000000 INFO @ Mon, 03 Jun 2019 13:20:21: 58000000 INFO @ Mon, 03 Jun 2019 13:20:24: 52000000 INFO @ Mon, 03 Jun 2019 13:20:25: 55000000 INFO @ Mon, 03 Jun 2019 13:20:27: 59000000 INFO @ Mon, 03 Jun 2019 13:20:31: 56000000 INFO @ Mon, 03 Jun 2019 13:20:31: 53000000 INFO @ Mon, 03 Jun 2019 13:20:32: 60000000 INFO @ Mon, 03 Jun 2019 13:20:37: 57000000 INFO @ Mon, 03 Jun 2019 13:20:37: 54000000 INFO @ Mon, 03 Jun 2019 13:20:38: 61000000 INFO @ Mon, 03 Jun 2019 13:20:42: 58000000 INFO @ Mon, 03 Jun 2019 13:20:43: 62000000 INFO @ Mon, 03 Jun 2019 13:20:44: 55000000 INFO @ Mon, 03 Jun 2019 13:20:48: 59000000 INFO @ Mon, 03 Jun 2019 13:20:48: 63000000 INFO @ Mon, 03 Jun 2019 13:20:51: 56000000 INFO @ Mon, 03 Jun 2019 13:20:54: 60000000 INFO @ Mon, 03 Jun 2019 13:20:54: 64000000 INFO @ Mon, 03 Jun 2019 13:20:57: 57000000 INFO @ Mon, 03 Jun 2019 13:20:59: 65000000 INFO @ Mon, 03 Jun 2019 13:20:59: 61000000 INFO @ Mon, 03 Jun 2019 13:21:04: 58000000 INFO @ Mon, 03 Jun 2019 13:21:05: 66000000 INFO @ Mon, 03 Jun 2019 13:21:05: 62000000 INFO @ Mon, 03 Jun 2019 13:21:10: 67000000 INFO @ Mon, 03 Jun 2019 13:21:10: 59000000 INFO @ Mon, 03 Jun 2019 13:21:11: 63000000 INFO @ Mon, 03 Jun 2019 13:21:15: 68000000 INFO @ Mon, 03 Jun 2019 13:21:17: 60000000 INFO @ Mon, 03 Jun 2019 13:21:17: 64000000 INFO @ Mon, 03 Jun 2019 13:21:21: 69000000 INFO @ Mon, 03 Jun 2019 13:21:22: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:21:22: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:21:22: #1 total tags in treatment: 32353959 INFO @ Mon, 03 Jun 2019 13:21:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:21:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:21:22: 65000000 INFO @ Mon, 03 Jun 2019 13:21:23: #1 tags after filtering in treatment: 25375572 INFO @ Mon, 03 Jun 2019 13:21:23: #1 Redundant rate of treatment: 0.22 INFO @ Mon, 03 Jun 2019 13:21:23: #1 finished! INFO @ Mon, 03 Jun 2019 13:21:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:21:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:21:23: 61000000 INFO @ Mon, 03 Jun 2019 13:21:25: #2 number of paired peaks: 28 WARNING @ Mon, 03 Jun 2019 13:21:25: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:21:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:21:28: 66000000 INFO @ Mon, 03 Jun 2019 13:21:30: 62000000 INFO @ Mon, 03 Jun 2019 13:21:34: 67000000 INFO @ Mon, 03 Jun 2019 13:21:36: 63000000 INFO @ Mon, 03 Jun 2019 13:21:39: 68000000 INFO @ Mon, 03 Jun 2019 13:21:43: 64000000 INFO @ Mon, 03 Jun 2019 13:21:45: 69000000 INFO @ Mon, 03 Jun 2019 13:21:46: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:21:46: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:21:46: #1 total tags in treatment: 32353959 INFO @ Mon, 03 Jun 2019 13:21:46: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:21:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:21:47: #1 tags after filtering in treatment: 25375572 INFO @ Mon, 03 Jun 2019 13:21:47: #1 Redundant rate of treatment: 0.22 INFO @ Mon, 03 Jun 2019 13:21:47: #1 finished! INFO @ Mon, 03 Jun 2019 13:21:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:21:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:21:49: 65000000 INFO @ Mon, 03 Jun 2019 13:21:49: #2 number of paired peaks: 28 WARNING @ Mon, 03 Jun 2019 13:21:49: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:21:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:21:55: 66000000 INFO @ Mon, 03 Jun 2019 13:22:02: 67000000 INFO @ Mon, 03 Jun 2019 13:22:08: 68000000 INFO @ Mon, 03 Jun 2019 13:22:15: 69000000 INFO @ Mon, 03 Jun 2019 13:22:16: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:22:16: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:22:16: #1 total tags in treatment: 32353959 INFO @ Mon, 03 Jun 2019 13:22:16: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:22:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:22:16: #1 tags after filtering in treatment: 25375572 INFO @ Mon, 03 Jun 2019 13:22:16: #1 Redundant rate of treatment: 0.22 INFO @ Mon, 03 Jun 2019 13:22:16: #1 finished! INFO @ Mon, 03 Jun 2019 13:22:16: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:22:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:22:19: #2 number of paired peaks: 28 WARNING @ Mon, 03 Jun 2019 13:22:19: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:22:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX301184/SRX301184.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。