Job ID = 1294907 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,033,854 reads read : 12,033,854 reads written : 12,033,854 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:14 12033854 reads; of these: 12033854 (100.00%) were unpaired; of these: 925029 (7.69%) aligned 0 times 9727357 (80.83%) aligned exactly 1 time 1381468 (11.48%) aligned >1 times 92.31% overall alignment rate Time searching: 00:03:14 Overall time: 00:03:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2430115 / 11108825 = 0.2188 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 10:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:51:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:51:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:51:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:51:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:51:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:51:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:51:31: 1000000 INFO @ Mon, 03 Jun 2019 10:51:32: 1000000 INFO @ Mon, 03 Jun 2019 10:51:32: 1000000 INFO @ Mon, 03 Jun 2019 10:51:38: 2000000 INFO @ Mon, 03 Jun 2019 10:51:40: 2000000 INFO @ Mon, 03 Jun 2019 10:51:40: 2000000 INFO @ Mon, 03 Jun 2019 10:51:45: 3000000 INFO @ Mon, 03 Jun 2019 10:51:48: 3000000 INFO @ Mon, 03 Jun 2019 10:51:48: 3000000 INFO @ Mon, 03 Jun 2019 10:51:52: 4000000 INFO @ Mon, 03 Jun 2019 10:51:55: 4000000 INFO @ Mon, 03 Jun 2019 10:51:56: 4000000 INFO @ Mon, 03 Jun 2019 10:51:59: 5000000 INFO @ Mon, 03 Jun 2019 10:52:03: 5000000 INFO @ Mon, 03 Jun 2019 10:52:03: 5000000 INFO @ Mon, 03 Jun 2019 10:52:06: 6000000 INFO @ Mon, 03 Jun 2019 10:52:11: 6000000 INFO @ Mon, 03 Jun 2019 10:52:11: 6000000 INFO @ Mon, 03 Jun 2019 10:52:13: 7000000 INFO @ Mon, 03 Jun 2019 10:52:19: 7000000 INFO @ Mon, 03 Jun 2019 10:52:19: 7000000 INFO @ Mon, 03 Jun 2019 10:52:20: 8000000 INFO @ Mon, 03 Jun 2019 10:52:24: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:52:24: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:52:24: #1 total tags in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:52:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:52:25: #1 tags after filtering in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:52:25: #1 finished! INFO @ Mon, 03 Jun 2019 10:52:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:52:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:52:25: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 10:52:25: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 10:52:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 10:52:27: 8000000 INFO @ Mon, 03 Jun 2019 10:52:27: 8000000 INFO @ Mon, 03 Jun 2019 10:52:32: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:52:32: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:52:32: #1 total tags in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:52:32: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:52:32: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:52:32: #1 total tags in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:52:32: #1 tags after filtering in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:52:32: #1 finished! INFO @ Mon, 03 Jun 2019 10:52:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:52:32: #1 tags after filtering in treatment: 8678710 INFO @ Mon, 03 Jun 2019 10:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:52:32: #1 finished! INFO @ Mon, 03 Jun 2019 10:52:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:52:33: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 10:52:33: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 10:52:33: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 10:52:33: #2 number of paired peaks: 30 WARNING @ Mon, 03 Jun 2019 10:52:33: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 10:52:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287981/SRX287981.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。