Job ID = 6527871 SRX = SRX287954 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:39:59 prefetch.2.10.7: 1) Downloading 'SRR870143'... 2020-06-29T13:39:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:42:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:42:33 prefetch.2.10.7: 1) 'SRR870143' was downloaded successfully Read 17177634 spots for SRR870143/SRR870143.sra Written 17177634 spots for SRR870143/SRR870143.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:17 17177634 reads; of these: 17177634 (100.00%) were unpaired; of these: 846886 (4.93%) aligned 0 times 14256775 (83.00%) aligned exactly 1 time 2073973 (12.07%) aligned >1 times 95.07% overall alignment rate Time searching: 00:04:17 Overall time: 00:04:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1596028 / 16330748 = 0.0977 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:55:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:55:55: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:55:55: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:56:02: 1000000 INFO @ Mon, 29 Jun 2020 22:56:08: 2000000 INFO @ Mon, 29 Jun 2020 22:56:14: 3000000 INFO @ Mon, 29 Jun 2020 22:56:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:56:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:56:25: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:56:25: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:56:27: 5000000 INFO @ Mon, 29 Jun 2020 22:56:32: 1000000 INFO @ Mon, 29 Jun 2020 22:56:33: 6000000 INFO @ Mon, 29 Jun 2020 22:56:39: 2000000 INFO @ Mon, 29 Jun 2020 22:56:40: 7000000 INFO @ Mon, 29 Jun 2020 22:56:46: 3000000 INFO @ Mon, 29 Jun 2020 22:56:47: 8000000 INFO @ Mon, 29 Jun 2020 22:56:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:56:54: 9000000 INFO @ Mon, 29 Jun 2020 22:56:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:56:57: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:56:57: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:56:59: 5000000 INFO @ Mon, 29 Jun 2020 22:57:01: 10000000 INFO @ Mon, 29 Jun 2020 22:57:04: 1000000 INFO @ Mon, 29 Jun 2020 22:57:06: 6000000 INFO @ Mon, 29 Jun 2020 22:57:07: 11000000 INFO @ Mon, 29 Jun 2020 22:57:11: 2000000 INFO @ Mon, 29 Jun 2020 22:57:13: 7000000 INFO @ Mon, 29 Jun 2020 22:57:14: 12000000 INFO @ Mon, 29 Jun 2020 22:57:18: 3000000 INFO @ Mon, 29 Jun 2020 22:57:20: 8000000 INFO @ Mon, 29 Jun 2020 22:57:21: 13000000 INFO @ Mon, 29 Jun 2020 22:57:25: 4000000 INFO @ Mon, 29 Jun 2020 22:57:27: 9000000 INFO @ Mon, 29 Jun 2020 22:57:28: 14000000 INFO @ Mon, 29 Jun 2020 22:57:32: 5000000 INFO @ Mon, 29 Jun 2020 22:57:33: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:57:33: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:57:33: #1 total tags in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:57:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:57:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:57:33: #1 tags after filtering in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:57:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:57:33: #1 finished! INFO @ Mon, 29 Jun 2020 22:57:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:57:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:57:34: 10000000 INFO @ Mon, 29 Jun 2020 22:57:34: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:57:34: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:57:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:57:39: 6000000 INFO @ Mon, 29 Jun 2020 22:57:41: 11000000 INFO @ Mon, 29 Jun 2020 22:57:45: 7000000 INFO @ Mon, 29 Jun 2020 22:57:48: 12000000 INFO @ Mon, 29 Jun 2020 22:57:52: 8000000 INFO @ Mon, 29 Jun 2020 22:57:55: 13000000 INFO @ Mon, 29 Jun 2020 22:57:59: 9000000 INFO @ Mon, 29 Jun 2020 22:58:02: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:58:06: 10000000 INFO @ Mon, 29 Jun 2020 22:58:07: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:58:07: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:58:07: #1 total tags in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:58:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:58:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:58:07: #1 tags after filtering in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:58:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:58:07: #1 finished! INFO @ Mon, 29 Jun 2020 22:58:07: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:58:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:58:08: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:58:08: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:58:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:58:13: 11000000 INFO @ Mon, 29 Jun 2020 22:58:19: 12000000 INFO @ Mon, 29 Jun 2020 22:58:26: 13000000 INFO @ Mon, 29 Jun 2020 22:58:32: 14000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 22:58:37: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:58:37: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:58:37: #1 total tags in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:58:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:58:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:58:37: #1 tags after filtering in treatment: 14734720 INFO @ Mon, 29 Jun 2020 22:58:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:58:37: #1 finished! INFO @ Mon, 29 Jun 2020 22:58:37: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:58:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:58:38: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 22:58:38: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:58:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287954/SRX287954.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling