Job ID = 6527869 SRX = SRX287936 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:49:03 prefetch.2.10.7: 1) Downloading 'SRR870125'... 2020-06-29T13:49:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:51:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:51:15 prefetch.2.10.7: 1) 'SRR870125' was downloaded successfully Read 17905413 spots for SRR870125/SRR870125.sra Written 17905413 spots for SRR870125/SRR870125.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:13 17905413 reads; of these: 17905413 (100.00%) were unpaired; of these: 1085082 (6.06%) aligned 0 times 11388824 (63.61%) aligned exactly 1 time 5431507 (30.33%) aligned >1 times 93.94% overall alignment rate Time searching: 00:06:13 Overall time: 00:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2440521 / 16820331 = 0.1451 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:56: 1000000 INFO @ Mon, 29 Jun 2020 23:07:01: 2000000 INFO @ Mon, 29 Jun 2020 23:07:06: 3000000 INFO @ Mon, 29 Jun 2020 23:07:11: 4000000 INFO @ Mon, 29 Jun 2020 23:07:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:07:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:07:21: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:07:21: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:22: 6000000 INFO @ Mon, 29 Jun 2020 23:07:27: 1000000 INFO @ Mon, 29 Jun 2020 23:07:27: 7000000 INFO @ Mon, 29 Jun 2020 23:07:32: 2000000 INFO @ Mon, 29 Jun 2020 23:07:33: 8000000 INFO @ Mon, 29 Jun 2020 23:07:38: 3000000 INFO @ Mon, 29 Jun 2020 23:07:38: 9000000 INFO @ Mon, 29 Jun 2020 23:07:43: 4000000 INFO @ Mon, 29 Jun 2020 23:07:44: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:07:49: 5000000 INFO @ Mon, 29 Jun 2020 23:07:50: 11000000 INFO @ Mon, 29 Jun 2020 23:07:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:07:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:07:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:54: 6000000 INFO @ Mon, 29 Jun 2020 23:07:55: 12000000 INFO @ Mon, 29 Jun 2020 23:07:56: 1000000 INFO @ Mon, 29 Jun 2020 23:08:00: 7000000 INFO @ Mon, 29 Jun 2020 23:08:01: 13000000 INFO @ Mon, 29 Jun 2020 23:08:02: 2000000 INFO @ Mon, 29 Jun 2020 23:08:05: 8000000 INFO @ Mon, 29 Jun 2020 23:08:06: 14000000 INFO @ Mon, 29 Jun 2020 23:08:07: 3000000 INFO @ Mon, 29 Jun 2020 23:08:08: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:08: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:08: #1 total tags in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:08:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:08: #1 tags after filtering in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:09: #2 number of paired peaks: 89 WARNING @ Mon, 29 Jun 2020 23:08:09: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:11: 9000000 INFO @ Mon, 29 Jun 2020 23:08:13: 4000000 INFO @ Mon, 29 Jun 2020 23:08:16: 10000000 INFO @ Mon, 29 Jun 2020 23:08:18: 5000000 INFO @ Mon, 29 Jun 2020 23:08:22: 11000000 INFO @ Mon, 29 Jun 2020 23:08:24: 6000000 INFO @ Mon, 29 Jun 2020 23:08:27: 12000000 INFO @ Mon, 29 Jun 2020 23:08:29: 7000000 INFO @ Mon, 29 Jun 2020 23:08:33: 13000000 INFO @ Mon, 29 Jun 2020 23:08:34: 8000000 INFO @ Mon, 29 Jun 2020 23:08:38: 14000000 INFO @ Mon, 29 Jun 2020 23:08:40: 9000000 INFO @ Mon, 29 Jun 2020 23:08:40: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:40: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:40: #1 total tags in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:08:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:40: #1 tags after filtering in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:08:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:40: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:40: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:41: #2 number of paired peaks: 89 WARNING @ Mon, 29 Jun 2020 23:08:41: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:45: 10000000 INFO @ Mon, 29 Jun 2020 23:08:50: 11000000 INFO @ Mon, 29 Jun 2020 23:08:56: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:09:01: 13000000 INFO @ Mon, 29 Jun 2020 23:09:06: 14000000 INFO @ Mon, 29 Jun 2020 23:09:08: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:09:08: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:09:08: #1 total tags in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:09:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:09:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:09:08: #1 tags after filtering in treatment: 14379810 INFO @ Mon, 29 Jun 2020 23:09:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:09:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:09:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:09:09: #2 number of paired peaks: 89 WARNING @ Mon, 29 Jun 2020 23:09:09: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:09:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287936/SRX287936.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。