Job ID = 6527860 SRX = SRX287895 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:04:21 prefetch.2.10.7: 1) Downloading 'SRR870084'... 2020-06-29T14:04:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:07:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:07:27 prefetch.2.10.7: 1) 'SRR870084' was downloaded successfully Read 18130031 spots for SRR870084/SRR870084.sra Written 18130031 spots for SRR870084/SRR870084.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:11 18130031 reads; of these: 18130031 (100.00%) were unpaired; of these: 1020959 (5.63%) aligned 0 times 12305529 (67.87%) aligned exactly 1 time 4803543 (26.49%) aligned >1 times 94.37% overall alignment rate Time searching: 00:07:11 Overall time: 00:07:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1806550 / 17109072 = 0.1056 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:08: 1000000 INFO @ Mon, 29 Jun 2020 23:25:14: 2000000 INFO @ Mon, 29 Jun 2020 23:25:20: 3000000 INFO @ Mon, 29 Jun 2020 23:25:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:32: 5000000 INFO @ Mon, 29 Jun 2020 23:25:39: 1000000 INFO @ Mon, 29 Jun 2020 23:25:40: 6000000 INFO @ Mon, 29 Jun 2020 23:25:46: 2000000 INFO @ Mon, 29 Jun 2020 23:25:47: 7000000 INFO @ Mon, 29 Jun 2020 23:25:53: 3000000 INFO @ Mon, 29 Jun 2020 23:25:54: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:26:00: 4000000 INFO @ Mon, 29 Jun 2020 23:26:00: 9000000 INFO @ Mon, 29 Jun 2020 23:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:26:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:26:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:26:07: 5000000 INFO @ Mon, 29 Jun 2020 23:26:07: 10000000 INFO @ Mon, 29 Jun 2020 23:26:09: 1000000 INFO @ Mon, 29 Jun 2020 23:26:14: 6000000 INFO @ Mon, 29 Jun 2020 23:26:14: 11000000 INFO @ Mon, 29 Jun 2020 23:26:16: 2000000 INFO @ Mon, 29 Jun 2020 23:26:21: 7000000 INFO @ Mon, 29 Jun 2020 23:26:21: 12000000 INFO @ Mon, 29 Jun 2020 23:26:23: 3000000 INFO @ Mon, 29 Jun 2020 23:26:28: 8000000 INFO @ Mon, 29 Jun 2020 23:26:28: 13000000 INFO @ Mon, 29 Jun 2020 23:26:30: 4000000 INFO @ Mon, 29 Jun 2020 23:26:35: 9000000 INFO @ Mon, 29 Jun 2020 23:26:35: 14000000 INFO @ Mon, 29 Jun 2020 23:26:38: 5000000 INFO @ Mon, 29 Jun 2020 23:26:41: 10000000 INFO @ Mon, 29 Jun 2020 23:26:42: 15000000 INFO @ Mon, 29 Jun 2020 23:26:44: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:26:44: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:26:44: #1 total tags in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:26:44: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:26:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:26:45: #1 tags after filtering in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:26:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:26:45: #1 finished! INFO @ Mon, 29 Jun 2020 23:26:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:26:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:26:45: 6000000 INFO @ Mon, 29 Jun 2020 23:26:46: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:26:46: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:26:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:26:48: 11000000 INFO @ Mon, 29 Jun 2020 23:26:52: 7000000 INFO @ Mon, 29 Jun 2020 23:26:55: 12000000 INFO @ Mon, 29 Jun 2020 23:26:59: 8000000 INFO @ Mon, 29 Jun 2020 23:27:02: 13000000 INFO @ Mon, 29 Jun 2020 23:27:06: 9000000 INFO @ Mon, 29 Jun 2020 23:27:08: 14000000 INFO @ Mon, 29 Jun 2020 23:27:13: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:27:15: 15000000 INFO @ Mon, 29 Jun 2020 23:27:17: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:27:17: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:27:17: #1 total tags in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:27:17: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:27:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:27:18: #1 tags after filtering in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:27:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:27:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:27:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:27:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:27:19: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:27:19: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:27:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:27:20: 11000000 INFO @ Mon, 29 Jun 2020 23:27:26: 12000000 INFO @ Mon, 29 Jun 2020 23:27:32: 13000000 INFO @ Mon, 29 Jun 2020 23:27:38: 14000000 INFO @ Mon, 29 Jun 2020 23:27:44: 15000000 INFO @ Mon, 29 Jun 2020 23:27:46: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:27:46: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:27:46: #1 total tags in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:27:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:27:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:27:47: #1 tags after filtering in treatment: 15302522 INFO @ Mon, 29 Jun 2020 23:27:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:27:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:27:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:27:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:27:48: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:27:48: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:27:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287895/SRX287895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。