Job ID = 6527828 SRX = SRX287787 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:35:37 prefetch.2.10.7: 1) Downloading 'SRR869976'... 2020-06-29T13:35:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:38:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:38:35 prefetch.2.10.7: 1) 'SRR869976' was downloaded successfully Read 18858986 spots for SRR869976/SRR869976.sra Written 18858986 spots for SRR869976/SRR869976.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:48 18858986 reads; of these: 18858986 (100.00%) were unpaired; of these: 1032071 (5.47%) aligned 0 times 14208689 (75.34%) aligned exactly 1 time 3618226 (19.19%) aligned >1 times 94.53% overall alignment rate Time searching: 00:05:48 Overall time: 00:05:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1779430 / 17826915 = 0.0998 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:57:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:57:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:57:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:57:08: 1000000 INFO @ Mon, 29 Jun 2020 22:57:15: 2000000 INFO @ Mon, 29 Jun 2020 22:57:21: 3000000 INFO @ Mon, 29 Jun 2020 22:57:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:57:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:57:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:57:33: 5000000 INFO @ Mon, 29 Jun 2020 22:57:39: 1000000 INFO @ Mon, 29 Jun 2020 22:57:40: 6000000 INFO @ Mon, 29 Jun 2020 22:57:46: 2000000 INFO @ Mon, 29 Jun 2020 22:57:46: 7000000 INFO @ Mon, 29 Jun 2020 22:57:52: 3000000 INFO @ Mon, 29 Jun 2020 22:57:53: 8000000 INFO @ Mon, 29 Jun 2020 22:57:59: 4000000 INFO @ Mon, 29 Jun 2020 22:57:59: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:58:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:58:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:58:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:58:06: 5000000 INFO @ Mon, 29 Jun 2020 22:58:06: 10000000 INFO @ Mon, 29 Jun 2020 22:58:09: 1000000 INFO @ Mon, 29 Jun 2020 22:58:13: 6000000 INFO @ Mon, 29 Jun 2020 22:58:13: 11000000 INFO @ Mon, 29 Jun 2020 22:58:15: 2000000 INFO @ Mon, 29 Jun 2020 22:58:20: 7000000 INFO @ Mon, 29 Jun 2020 22:58:20: 12000000 INFO @ Mon, 29 Jun 2020 22:58:21: 3000000 INFO @ Mon, 29 Jun 2020 22:58:26: 8000000 INFO @ Mon, 29 Jun 2020 22:58:27: 13000000 INFO @ Mon, 29 Jun 2020 22:58:28: 4000000 INFO @ Mon, 29 Jun 2020 22:58:33: 9000000 INFO @ Mon, 29 Jun 2020 22:58:34: 14000000 INFO @ Mon, 29 Jun 2020 22:58:34: 5000000 INFO @ Mon, 29 Jun 2020 22:58:40: 10000000 INFO @ Mon, 29 Jun 2020 22:58:40: 15000000 INFO @ Mon, 29 Jun 2020 22:58:41: 6000000 INFO @ Mon, 29 Jun 2020 22:58:46: 11000000 INFO @ Mon, 29 Jun 2020 22:58:47: 16000000 INFO @ Mon, 29 Jun 2020 22:58:47: 7000000 INFO @ Mon, 29 Jun 2020 22:58:48: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:58:48: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:58:48: #1 total tags in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:58:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:58:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:58:48: #1 tags after filtering in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:58:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:58:48: #1 finished! INFO @ Mon, 29 Jun 2020 22:58:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:58:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:58:49: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 22:58:49: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:58:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:58:53: 12000000 INFO @ Mon, 29 Jun 2020 22:58:54: 8000000 INFO @ Mon, 29 Jun 2020 22:58:59: 13000000 INFO @ Mon, 29 Jun 2020 22:59:00: 9000000 INFO @ Mon, 29 Jun 2020 22:59:05: 14000000 INFO @ Mon, 29 Jun 2020 22:59:06: 10000000 INFO @ Mon, 29 Jun 2020 22:59:12: 15000000 INFO @ Mon, 29 Jun 2020 22:59:12: 11000000 INFO @ Mon, 29 Jun 2020 22:59:18: 16000000 INFO @ Mon, 29 Jun 2020 22:59:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:59:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:59:18: #1 total tags in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:59:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:59:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:59:19: #1 tags after filtering in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:59:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:59:19: #1 finished! INFO @ Mon, 29 Jun 2020 22:59:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:59:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:59:19: 12000000 INFO @ Mon, 29 Jun 2020 22:59:20: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 22:59:20: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:59:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:59:25: 13000000 INFO @ Mon, 29 Jun 2020 22:59:32: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:59:38: 15000000 INFO @ Mon, 29 Jun 2020 22:59:44: 16000000 INFO @ Mon, 29 Jun 2020 22:59:44: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:59:44: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:59:44: #1 total tags in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:59:44: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:59:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:59:45: #1 tags after filtering in treatment: 16047485 INFO @ Mon, 29 Jun 2020 22:59:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:59:45: #1 finished! INFO @ Mon, 29 Jun 2020 22:59:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:59:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:59:46: #2 number of paired peaks: 58 WARNING @ Mon, 29 Jun 2020 22:59:46: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:59:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287787/SRX287787.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。