Job ID = 6527826 SRX = SRX287779 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:56:14 prefetch.2.10.7: 1) Downloading 'SRR869968'... 2020-06-29T13:56:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:00:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:00:10 prefetch.2.10.7: 1) 'SRR869968' was downloaded successfully Read 22465616 spots for SRR869968/SRR869968.sra Written 22465616 spots for SRR869968/SRR869968.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:13 22465616 reads; of these: 22465616 (100.00%) were unpaired; of these: 1899667 (8.46%) aligned 0 times 16477598 (73.35%) aligned exactly 1 time 4088351 (18.20%) aligned >1 times 91.54% overall alignment rate Time searching: 00:07:13 Overall time: 00:07:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3009577 / 20565949 = 0.1463 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:21:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:21:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:21:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:22:06: 1000000 INFO @ Mon, 29 Jun 2020 23:22:12: 2000000 INFO @ Mon, 29 Jun 2020 23:22:18: 3000000 INFO @ Mon, 29 Jun 2020 23:22:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:22:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:22:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:22:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:22:31: 5000000 INFO @ Mon, 29 Jun 2020 23:22:36: 1000000 INFO @ Mon, 29 Jun 2020 23:22:38: 6000000 INFO @ Mon, 29 Jun 2020 23:22:43: 2000000 INFO @ Mon, 29 Jun 2020 23:22:44: 7000000 INFO @ Mon, 29 Jun 2020 23:22:50: 3000000 INFO @ Mon, 29 Jun 2020 23:22:51: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:22:57: 4000000 INFO @ Mon, 29 Jun 2020 23:22:58: 9000000 INFO @ Mon, 29 Jun 2020 23:22:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:22:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:22:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:23:04: 5000000 INFO @ Mon, 29 Jun 2020 23:23:05: 10000000 INFO @ Mon, 29 Jun 2020 23:23:06: 1000000 INFO @ Mon, 29 Jun 2020 23:23:11: 6000000 INFO @ Mon, 29 Jun 2020 23:23:12: 11000000 INFO @ Mon, 29 Jun 2020 23:23:13: 2000000 INFO @ Mon, 29 Jun 2020 23:23:19: 7000000 INFO @ Mon, 29 Jun 2020 23:23:19: 12000000 INFO @ Mon, 29 Jun 2020 23:23:21: 3000000 INFO @ Mon, 29 Jun 2020 23:23:26: 8000000 INFO @ Mon, 29 Jun 2020 23:23:26: 13000000 INFO @ Mon, 29 Jun 2020 23:23:28: 4000000 INFO @ Mon, 29 Jun 2020 23:23:33: 9000000 INFO @ Mon, 29 Jun 2020 23:23:34: 14000000 INFO @ Mon, 29 Jun 2020 23:23:35: 5000000 INFO @ Mon, 29 Jun 2020 23:23:41: 10000000 INFO @ Mon, 29 Jun 2020 23:23:41: 15000000 INFO @ Mon, 29 Jun 2020 23:23:42: 6000000 INFO @ Mon, 29 Jun 2020 23:23:48: 11000000 INFO @ Mon, 29 Jun 2020 23:23:48: 16000000 INFO @ Mon, 29 Jun 2020 23:23:49: 7000000 INFO @ Mon, 29 Jun 2020 23:23:55: 17000000 INFO @ Mon, 29 Jun 2020 23:23:55: 12000000 INFO @ Mon, 29 Jun 2020 23:23:56: 8000000 INFO @ Mon, 29 Jun 2020 23:23:59: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:23:59: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:23:59: #1 total tags in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:23:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:23:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:24:00: #1 tags after filtering in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:24:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:24:00: #1 finished! INFO @ Mon, 29 Jun 2020 23:24:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:24:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:24:01: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:24:01: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:24:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:24:03: 13000000 INFO @ Mon, 29 Jun 2020 23:24:03: 9000000 INFO @ Mon, 29 Jun 2020 23:24:10: 14000000 INFO @ Mon, 29 Jun 2020 23:24:10: 10000000 INFO @ Mon, 29 Jun 2020 23:24:17: 15000000 INFO @ Mon, 29 Jun 2020 23:24:17: 11000000 INFO @ Mon, 29 Jun 2020 23:24:23: 12000000 INFO @ Mon, 29 Jun 2020 23:24:24: 16000000 INFO @ Mon, 29 Jun 2020 23:24:30: 13000000 INFO @ Mon, 29 Jun 2020 23:24:30: 17000000 INFO @ Mon, 29 Jun 2020 23:24:34: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:24:34: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:24:34: #1 total tags in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:24:34: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:24:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:24:35: #1 tags after filtering in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:24:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:24:35: #1 finished! INFO @ Mon, 29 Jun 2020 23:24:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:24:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:24:36: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:24:36: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:24:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:24:37: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:24:43: 15000000 INFO @ Mon, 29 Jun 2020 23:24:49: 16000000 INFO @ Mon, 29 Jun 2020 23:24:55: 17000000 INFO @ Mon, 29 Jun 2020 23:24:59: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:24:59: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:24:59: #1 total tags in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:24:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:24:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:24:59: #1 tags after filtering in treatment: 17556372 INFO @ Mon, 29 Jun 2020 23:24:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:24:59: #1 finished! INFO @ Mon, 29 Jun 2020 23:24:59: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:24:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:25:00: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:25:00: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:25:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287779/SRX287779.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。