Job ID = 1294549 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,108,114 reads read : 19,108,114 reads written : 19,108,114 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:57 19108114 reads; of these: 19108114 (100.00%) were unpaired; of these: 1086724 (5.69%) aligned 0 times 16069031 (84.10%) aligned exactly 1 time 1952359 (10.22%) aligned >1 times 94.31% overall alignment rate Time searching: 00:04:57 Overall time: 00:04:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3347250 / 18021390 = 0.1857 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 09:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:05:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:05:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:05:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:05:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:05:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:05:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:05:34: 1000000 INFO @ Mon, 03 Jun 2019 09:05:34: 1000000 INFO @ Mon, 03 Jun 2019 09:05:35: 1000000 INFO @ Mon, 03 Jun 2019 09:05:43: 2000000 INFO @ Mon, 03 Jun 2019 09:05:43: 2000000 INFO @ Mon, 03 Jun 2019 09:05:43: 2000000 INFO @ Mon, 03 Jun 2019 09:05:50: 3000000 INFO @ Mon, 03 Jun 2019 09:05:50: 3000000 INFO @ Mon, 03 Jun 2019 09:05:50: 3000000 INFO @ Mon, 03 Jun 2019 09:05:58: 4000000 INFO @ Mon, 03 Jun 2019 09:05:58: 4000000 INFO @ Mon, 03 Jun 2019 09:05:58: 4000000 INFO @ Mon, 03 Jun 2019 09:06:06: 5000000 INFO @ Mon, 03 Jun 2019 09:06:06: 5000000 INFO @ Mon, 03 Jun 2019 09:06:06: 5000000 INFO @ Mon, 03 Jun 2019 09:06:14: 6000000 INFO @ Mon, 03 Jun 2019 09:06:14: 6000000 INFO @ Mon, 03 Jun 2019 09:06:14: 6000000 INFO @ Mon, 03 Jun 2019 09:06:22: 7000000 INFO @ Mon, 03 Jun 2019 09:06:22: 7000000 INFO @ Mon, 03 Jun 2019 09:06:22: 7000000 INFO @ Mon, 03 Jun 2019 09:06:29: 8000000 INFO @ Mon, 03 Jun 2019 09:06:30: 8000000 INFO @ Mon, 03 Jun 2019 09:06:30: 8000000 INFO @ Mon, 03 Jun 2019 09:06:37: 9000000 INFO @ Mon, 03 Jun 2019 09:06:37: 9000000 INFO @ Mon, 03 Jun 2019 09:06:37: 9000000 INFO @ Mon, 03 Jun 2019 09:06:45: 10000000 INFO @ Mon, 03 Jun 2019 09:06:45: 10000000 INFO @ Mon, 03 Jun 2019 09:06:45: 10000000 INFO @ Mon, 03 Jun 2019 09:06:52: 11000000 INFO @ Mon, 03 Jun 2019 09:06:53: 11000000 INFO @ Mon, 03 Jun 2019 09:06:53: 11000000 INFO @ Mon, 03 Jun 2019 09:07:00: 12000000 INFO @ Mon, 03 Jun 2019 09:07:01: 12000000 INFO @ Mon, 03 Jun 2019 09:07:01: 12000000 INFO @ Mon, 03 Jun 2019 09:07:08: 13000000 INFO @ Mon, 03 Jun 2019 09:07:08: 13000000 INFO @ Mon, 03 Jun 2019 09:07:08: 13000000 INFO @ Mon, 03 Jun 2019 09:07:15: 14000000 INFO @ Mon, 03 Jun 2019 09:07:16: 14000000 INFO @ Mon, 03 Jun 2019 09:07:16: 14000000 INFO @ Mon, 03 Jun 2019 09:07:21: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 09:07:21: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 09:07:21: #1 total tags in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:07:21: #1 tags after filtering in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 09:07:21: #1 finished! INFO @ Mon, 03 Jun 2019 09:07:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:07:22: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 09:07:22: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 09:07:22: #1 total tags in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:07:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:07:22: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 09:07:22: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 09:07:22: #1 total tags in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:07:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:07:22: #1 tags after filtering in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 09:07:22: #1 finished! INFO @ Mon, 03 Jun 2019 09:07:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:07:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:07:22: #1 tags after filtering in treatment: 14674140 INFO @ Mon, 03 Jun 2019 09:07:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 09:07:22: #1 finished! INFO @ Mon, 03 Jun 2019 09:07:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:07:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:07:22: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 09:07:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 09:07:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 09:07:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 09:07:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 09:07:23: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 09:07:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 09:07:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 09:07:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287735/SRX287735.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。