Job ID = 6527814 SRX = SRX287722 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:27:34 prefetch.2.10.7: 1) Downloading 'SRR869911'... 2020-06-29T13:27:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:30:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:30:29 prefetch.2.10.7: 1) 'SRR869911' was downloaded successfully Read 18393353 spots for SRR869911/SRR869911.sra Written 18393353 spots for SRR869911/SRR869911.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:37 18393353 reads; of these: 18393353 (100.00%) were unpaired; of these: 1113952 (6.06%) aligned 0 times 13363638 (72.65%) aligned exactly 1 time 3915763 (21.29%) aligned >1 times 93.94% overall alignment rate Time searching: 00:05:37 Overall time: 00:05:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1105951 / 17279401 = 0.0640 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:45:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:45:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:45:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:45:59: 1000000 INFO @ Mon, 29 Jun 2020 22:46:06: 2000000 INFO @ Mon, 29 Jun 2020 22:46:13: 3000000 INFO @ Mon, 29 Jun 2020 22:46:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:46:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:46:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:46:26: 5000000 INFO @ Mon, 29 Jun 2020 22:46:29: 1000000 INFO @ Mon, 29 Jun 2020 22:46:33: 6000000 INFO @ Mon, 29 Jun 2020 22:46:36: 2000000 INFO @ Mon, 29 Jun 2020 22:46:40: 7000000 INFO @ Mon, 29 Jun 2020 22:46:43: 3000000 INFO @ Mon, 29 Jun 2020 22:46:47: 8000000 INFO @ Mon, 29 Jun 2020 22:46:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:46:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:46:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:46:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:46:55: 9000000 INFO @ Mon, 29 Jun 2020 22:46:57: 5000000 INFO @ Mon, 29 Jun 2020 22:46:59: 1000000 INFO @ Mon, 29 Jun 2020 22:47:02: 10000000 INFO @ Mon, 29 Jun 2020 22:47:05: 6000000 INFO @ Mon, 29 Jun 2020 22:47:06: 2000000 INFO @ Mon, 29 Jun 2020 22:47:09: 11000000 INFO @ Mon, 29 Jun 2020 22:47:12: 7000000 INFO @ Mon, 29 Jun 2020 22:47:13: 3000000 INFO @ Mon, 29 Jun 2020 22:47:17: 12000000 INFO @ Mon, 29 Jun 2020 22:47:19: 4000000 INFO @ Mon, 29 Jun 2020 22:47:19: 8000000 INFO @ Mon, 29 Jun 2020 22:47:25: 13000000 INFO @ Mon, 29 Jun 2020 22:47:26: 5000000 INFO @ Mon, 29 Jun 2020 22:47:27: 9000000 INFO @ Mon, 29 Jun 2020 22:47:32: 14000000 INFO @ Mon, 29 Jun 2020 22:47:33: 6000000 INFO @ Mon, 29 Jun 2020 22:47:34: 10000000 INFO @ Mon, 29 Jun 2020 22:47:40: 7000000 INFO @ Mon, 29 Jun 2020 22:47:40: 15000000 INFO @ Mon, 29 Jun 2020 22:47:41: 11000000 INFO @ Mon, 29 Jun 2020 22:47:46: 8000000 INFO @ Mon, 29 Jun 2020 22:47:48: 16000000 INFO @ Mon, 29 Jun 2020 22:47:49: 12000000 INFO @ Mon, 29 Jun 2020 22:47:49: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:47:49: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:47:49: #1 total tags in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:47:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:47:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:47:49: #1 tags after filtering in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:47:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:47:49: #1 finished! INFO @ Mon, 29 Jun 2020 22:47:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:47:50: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:47:50: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:47:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:47:53: 9000000 INFO @ Mon, 29 Jun 2020 22:47:56: 13000000 INFO @ Mon, 29 Jun 2020 22:48:00: 10000000 INFO @ Mon, 29 Jun 2020 22:48:04: 14000000 INFO @ Mon, 29 Jun 2020 22:48:06: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:48:12: 15000000 INFO @ Mon, 29 Jun 2020 22:48:13: 12000000 INFO @ Mon, 29 Jun 2020 22:48:19: 16000000 INFO @ Mon, 29 Jun 2020 22:48:20: 13000000 INFO @ Mon, 29 Jun 2020 22:48:20: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:48:20: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:48:20: #1 total tags in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:48:20: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:48:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:48:20: #1 tags after filtering in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:48:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:48:20: #1 finished! INFO @ Mon, 29 Jun 2020 22:48:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:48:21: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:48:21: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:48:26: 14000000 INFO @ Mon, 29 Jun 2020 22:48:33: 15000000 INFO @ Mon, 29 Jun 2020 22:48:39: 16000000 INFO @ Mon, 29 Jun 2020 22:48:40: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:48:40: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:48:40: #1 total tags in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:48:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:48:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:48:41: #1 tags after filtering in treatment: 16173450 INFO @ Mon, 29 Jun 2020 22:48:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:48:41: #1 finished! INFO @ Mon, 29 Jun 2020 22:48:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:48:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:48:42: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:48:42: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:48:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287722/SRX287722.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。