Job ID = 6527802 SRX = SRX287677 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:29:55 prefetch.2.10.7: 1) Downloading 'SRR869865'... 2020-06-29T13:29:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:32:31 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:32:31 prefetch.2.10.7: 1) 'SRR869865' was downloaded successfully Read 18412477 spots for SRR869865/SRR869865.sra Written 18412477 spots for SRR869865/SRR869865.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:15 18412477 reads; of these: 18412477 (100.00%) were unpaired; of these: 986711 (5.36%) aligned 0 times 13569757 (73.70%) aligned exactly 1 time 3856009 (20.94%) aligned >1 times 94.64% overall alignment rate Time searching: 00:06:16 Overall time: 00:06:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1205272 / 17425766 = 0.0692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:51:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:51:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:51:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:51:39: 1000000 INFO @ Mon, 29 Jun 2020 22:51:46: 2000000 INFO @ Mon, 29 Jun 2020 22:51:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:52:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:52:00: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:52:00: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:52:01: 4000000 INFO @ Mon, 29 Jun 2020 22:52:09: 1000000 INFO @ Mon, 29 Jun 2020 22:52:10: 5000000 INFO @ Mon, 29 Jun 2020 22:52:17: 2000000 INFO @ Mon, 29 Jun 2020 22:52:18: 6000000 INFO @ Mon, 29 Jun 2020 22:52:25: 3000000 INFO @ Mon, 29 Jun 2020 22:52:26: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:52:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:52:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:52:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:52:33: 4000000 INFO @ Mon, 29 Jun 2020 22:52:34: 8000000 INFO @ Mon, 29 Jun 2020 22:52:39: 1000000 INFO @ Mon, 29 Jun 2020 22:52:42: 5000000 INFO @ Mon, 29 Jun 2020 22:52:43: 9000000 INFO @ Mon, 29 Jun 2020 22:52:47: 2000000 INFO @ Mon, 29 Jun 2020 22:52:50: 6000000 INFO @ Mon, 29 Jun 2020 22:52:51: 10000000 INFO @ Mon, 29 Jun 2020 22:52:56: 3000000 INFO @ Mon, 29 Jun 2020 22:52:59: 7000000 INFO @ Mon, 29 Jun 2020 22:53:00: 11000000 INFO @ Mon, 29 Jun 2020 22:53:04: 4000000 INFO @ Mon, 29 Jun 2020 22:53:07: 8000000 INFO @ Mon, 29 Jun 2020 22:53:08: 12000000 INFO @ Mon, 29 Jun 2020 22:53:13: 5000000 INFO @ Mon, 29 Jun 2020 22:53:16: 9000000 INFO @ Mon, 29 Jun 2020 22:53:17: 13000000 INFO @ Mon, 29 Jun 2020 22:53:21: 6000000 INFO @ Mon, 29 Jun 2020 22:53:24: 10000000 INFO @ Mon, 29 Jun 2020 22:53:26: 14000000 INFO @ Mon, 29 Jun 2020 22:53:30: 7000000 INFO @ Mon, 29 Jun 2020 22:53:33: 11000000 INFO @ Mon, 29 Jun 2020 22:53:35: 15000000 INFO @ Mon, 29 Jun 2020 22:53:38: 8000000 INFO @ Mon, 29 Jun 2020 22:53:41: 12000000 INFO @ Mon, 29 Jun 2020 22:53:43: 16000000 INFO @ Mon, 29 Jun 2020 22:53:45: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:53:45: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:53:45: #1 total tags in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:53:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:53:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:53:45: #1 tags after filtering in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:53:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:53:45: #1 finished! INFO @ Mon, 29 Jun 2020 22:53:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:53:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:53:46: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 22:53:46: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:53:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05_peaks.narrowPeak: No such file or directory INFO @ Mon, 29 Jun 2020 22:53:47: 9000000 pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:53:50: 13000000 INFO @ Mon, 29 Jun 2020 22:53:55: 10000000 INFO @ Mon, 29 Jun 2020 22:53:58: 14000000 INFO @ Mon, 29 Jun 2020 22:54:03: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:54:07: 15000000 INFO @ Mon, 29 Jun 2020 22:54:11: 12000000 INFO @ Mon, 29 Jun 2020 22:54:15: 16000000 INFO @ Mon, 29 Jun 2020 22:54:17: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:54:17: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:54:17: #1 total tags in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:54:17: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:54:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:54:17: #1 tags after filtering in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:54:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:54:17: #1 finished! INFO @ Mon, 29 Jun 2020 22:54:17: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:54:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:54:18: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 22:54:18: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:54:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:54:19: 13000000 INFO @ Mon, 29 Jun 2020 22:54:26: 14000000 INFO @ Mon, 29 Jun 2020 22:54:34: 15000000 INFO @ Mon, 29 Jun 2020 22:54:41: 16000000 INFO @ Mon, 29 Jun 2020 22:54:43: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:54:43: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:54:43: #1 total tags in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:54:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:54:43: #1 tags after filtering in treatment: 16220494 INFO @ Mon, 29 Jun 2020 22:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:54:43: #1 finished! INFO @ Mon, 29 Jun 2020 22:54:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:54:44: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 22:54:44: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:54:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287677/SRX287677.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。