Job ID = 6527796 SRX = SRX287605 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:25:37 prefetch.2.10.7: 1) Downloading 'SRR869748'... 2020-06-29T13:25:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:28:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:28:12 prefetch.2.10.7: 1) 'SRR869748' was downloaded successfully Read 18523762 spots for SRR869748/SRR869748.sra Written 18523762 spots for SRR869748/SRR869748.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:14 18523762 reads; of these: 18523762 (100.00%) were unpaired; of these: 1223789 (6.61%) aligned 0 times 15890072 (85.78%) aligned exactly 1 time 1409901 (7.61%) aligned >1 times 93.39% overall alignment rate Time searching: 00:04:14 Overall time: 00:04:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6101284 / 17299973 = 0.3527 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:41:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:41:43: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:41:43: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:41:49: 1000000 INFO @ Mon, 29 Jun 2020 22:41:55: 2000000 INFO @ Mon, 29 Jun 2020 22:42:01: 3000000 INFO @ Mon, 29 Jun 2020 22:42:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:14: 5000000 INFO @ Mon, 29 Jun 2020 22:42:19: 1000000 INFO @ Mon, 29 Jun 2020 22:42:20: 6000000 INFO @ Mon, 29 Jun 2020 22:42:26: 2000000 INFO @ Mon, 29 Jun 2020 22:42:27: 7000000 INFO @ Mon, 29 Jun 2020 22:42:32: 3000000 INFO @ Mon, 29 Jun 2020 22:42:33: 8000000 INFO @ Mon, 29 Jun 2020 22:42:39: 4000000 INFO @ Mon, 29 Jun 2020 22:42:40: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:43: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:43: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:45: 5000000 INFO @ Mon, 29 Jun 2020 22:42:46: 10000000 INFO @ Mon, 29 Jun 2020 22:42:49: 1000000 INFO @ Mon, 29 Jun 2020 22:42:52: 6000000 INFO @ Mon, 29 Jun 2020 22:42:53: 11000000 INFO @ Mon, 29 Jun 2020 22:42:54: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:42:54: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:42:54: #1 total tags in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:42:54: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:42:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:42:54: #1 tags after filtering in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:42:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:42:54: #1 finished! INFO @ Mon, 29 Jun 2020 22:42:54: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:42:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:42:55: #2 number of paired peaks: 70 WARNING @ Mon, 29 Jun 2020 22:42:55: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:42:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:42:56: 2000000 INFO @ Mon, 29 Jun 2020 22:42:58: 7000000 INFO @ Mon, 29 Jun 2020 22:43:02: 3000000 INFO @ Mon, 29 Jun 2020 22:43:05: 8000000 INFO @ Mon, 29 Jun 2020 22:43:08: 4000000 INFO @ Mon, 29 Jun 2020 22:43:11: 9000000 INFO @ Mon, 29 Jun 2020 22:43:15: 5000000 INFO @ Mon, 29 Jun 2020 22:43:17: 10000000 INFO @ Mon, 29 Jun 2020 22:43:21: 6000000 INFO @ Mon, 29 Jun 2020 22:43:24: 11000000 INFO @ Mon, 29 Jun 2020 22:43:25: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:43:25: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:43:25: #1 total tags in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:43:25: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:25: #1 tags after filtering in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:43:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:25: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:26: #2 number of paired peaks: 70 WARNING @ Mon, 29 Jun 2020 22:43:26: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:43:27: 7000000 INFO @ Mon, 29 Jun 2020 22:43:33: 8000000 INFO @ Mon, 29 Jun 2020 22:43:39: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:43:45: 10000000 INFO @ Mon, 29 Jun 2020 22:43:51: 11000000 INFO @ Mon, 29 Jun 2020 22:43:53: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:43:53: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:43:53: #1 total tags in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:43:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:53: #1 tags after filtering in treatment: 11198689 INFO @ Mon, 29 Jun 2020 22:43:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:53: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:53: #2 number of paired peaks: 70 WARNING @ Mon, 29 Jun 2020 22:43:53: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287605/SRX287605.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。