Job ID = 10175251 sra ファイルのダウンロード中... Completed: 552389K bytes transferred in 15 seconds (284611K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 31202456 spots for /home/okishinya/chipatlas/results/dm3/SRX2804229/SRR5534657.sra Written 31202456 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:14:05 31202456 reads; of these: 31202456 (100.00%) were unpaired; of these: 2381215 (7.63%) aligned 0 times 18907424 (60.60%) aligned exactly 1 time 9913817 (31.77%) aligned >1 times 92.37% overall alignment rate Time searching: 00:14:08 Overall time: 00:14:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3824806 / 28821241 = 0.1327 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Nov 2017 12:22:37: # Command line: callpeak -t SRX2804229.bam -f BAM -g dm -n SRX2804229.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2804229.10 # format = BAM # ChIP-seq file = ['SRX2804229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:22:37: # Command line: callpeak -t SRX2804229.bam -f BAM -g dm -n SRX2804229.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2804229.20 # format = BAM # ChIP-seq file = ['SRX2804229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:22:37: # Command line: callpeak -t SRX2804229.bam -f BAM -g dm -n SRX2804229.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2804229.05 # format = BAM # ChIP-seq file = ['SRX2804229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Nov 2017 12:22:37: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:22:37: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:22:37: #1 read tag files... INFO @ Mon, 06 Nov 2017 12:22:37: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:22:37: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:22:37: #1 read treatment tags... INFO @ Mon, 06 Nov 2017 12:22:44: 1000000 INFO @ Mon, 06 Nov 2017 12:22:44: 1000000 INFO @ Mon, 06 Nov 2017 12:22:44: 1000000 INFO @ Mon, 06 Nov 2017 12:22:51: 2000000 INFO @ Mon, 06 Nov 2017 12:22:51: 2000000 INFO @ Mon, 06 Nov 2017 12:22:51: 2000000 INFO @ Mon, 06 Nov 2017 12:22:58: 3000000 INFO @ Mon, 06 Nov 2017 12:22:58: 3000000 INFO @ Mon, 06 Nov 2017 12:22:58: 3000000 INFO @ Mon, 06 Nov 2017 12:23:04: 4000000 INFO @ Mon, 06 Nov 2017 12:23:04: 4000000 INFO @ Mon, 06 Nov 2017 12:23:05: 4000000 INFO @ Mon, 06 Nov 2017 12:23:11: 5000000 INFO @ Mon, 06 Nov 2017 12:23:11: 5000000 INFO @ Mon, 06 Nov 2017 12:23:12: 5000000 INFO @ Mon, 06 Nov 2017 12:23:18: 6000000 INFO @ Mon, 06 Nov 2017 12:23:18: 6000000 INFO @ Mon, 06 Nov 2017 12:23:19: 6000000 INFO @ Mon, 06 Nov 2017 12:23:25: 7000000 INFO @ Mon, 06 Nov 2017 12:23:25: 7000000 INFO @ Mon, 06 Nov 2017 12:23:27: 7000000 INFO @ Mon, 06 Nov 2017 12:23:31: 8000000 INFO @ Mon, 06 Nov 2017 12:23:32: 8000000 INFO @ Mon, 06 Nov 2017 12:23:34: 8000000 INFO @ Mon, 06 Nov 2017 12:23:38: 9000000 INFO @ Mon, 06 Nov 2017 12:23:39: 9000000 INFO @ Mon, 06 Nov 2017 12:23:41: 9000000 INFO @ Mon, 06 Nov 2017 12:23:45: 10000000 INFO @ Mon, 06 Nov 2017 12:23:46: 10000000 INFO @ Mon, 06 Nov 2017 12:23:48: 10000000 INFO @ Mon, 06 Nov 2017 12:23:52: 11000000 INFO @ Mon, 06 Nov 2017 12:23:53: 11000000 INFO @ Mon, 06 Nov 2017 12:23:55: 11000000 INFO @ Mon, 06 Nov 2017 12:23:59: 12000000 INFO @ Mon, 06 Nov 2017 12:24:00: 12000000 INFO @ Mon, 06 Nov 2017 12:24:02: 12000000 INFO @ Mon, 06 Nov 2017 12:24:06: 13000000 INFO @ Mon, 06 Nov 2017 12:24:08: 13000000 INFO @ Mon, 06 Nov 2017 12:24:10: 13000000 INFO @ Mon, 06 Nov 2017 12:24:13: 14000000 INFO @ Mon, 06 Nov 2017 12:24:15: 14000000 INFO @ Mon, 06 Nov 2017 12:24:17: 14000000 INFO @ Mon, 06 Nov 2017 12:24:20: 15000000 INFO @ Mon, 06 Nov 2017 12:24:23: 15000000 INFO @ Mon, 06 Nov 2017 12:24:24: 15000000 INFO @ Mon, 06 Nov 2017 12:24:26: 16000000 INFO @ Mon, 06 Nov 2017 12:24:30: 16000000 INFO @ Mon, 06 Nov 2017 12:24:32: 16000000 INFO @ Mon, 06 Nov 2017 12:24:33: 17000000 INFO @ Mon, 06 Nov 2017 12:24:37: 17000000 INFO @ Mon, 06 Nov 2017 12:24:39: 17000000 INFO @ Mon, 06 Nov 2017 12:24:40: 18000000 INFO @ Mon, 06 Nov 2017 12:24:45: 18000000 INFO @ Mon, 06 Nov 2017 12:24:47: 18000000 INFO @ Mon, 06 Nov 2017 12:24:47: 19000000 INFO @ Mon, 06 Nov 2017 12:24:53: 19000000 INFO @ Mon, 06 Nov 2017 12:24:54: 20000000 INFO @ Mon, 06 Nov 2017 12:24:54: 19000000 INFO @ Mon, 06 Nov 2017 12:25:00: 20000000 INFO @ Mon, 06 Nov 2017 12:25:01: 21000000 INFO @ Mon, 06 Nov 2017 12:25:02: 20000000 INFO @ Mon, 06 Nov 2017 12:25:08: 22000000 INFO @ Mon, 06 Nov 2017 12:25:08: 21000000 INFO @ Mon, 06 Nov 2017 12:25:09: 21000000 INFO @ Mon, 06 Nov 2017 12:25:14: 23000000 INFO @ Mon, 06 Nov 2017 12:25:16: 22000000 INFO @ Mon, 06 Nov 2017 12:25:17: 22000000 INFO @ Mon, 06 Nov 2017 12:25:21: 24000000 INFO @ Mon, 06 Nov 2017 12:25:23: 23000000 INFO @ Mon, 06 Nov 2017 12:25:25: 23000000 INFO @ Mon, 06 Nov 2017 12:25:28: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:25:28: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:25:28: #1 total tags in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:28: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:25:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:25:29: #1 tags after filtering in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:25:29: #1 finished! INFO @ Mon, 06 Nov 2017 12:25:29: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:25:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:25:30: #2 number of paired peaks: 94 WARNING @ Mon, 06 Nov 2017 12:25:30: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:25:30: Process for pairing-model is terminated! cat: SRX2804229.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2804229.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Nov 2017 12:25:31: 24000000 INFO @ Mon, 06 Nov 2017 12:25:32: 24000000 INFO @ Mon, 06 Nov 2017 12:25:38: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:25:38: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:25:38: #1 total tags in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:38: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:25:38: #1 tags after filtering in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:25:38: #1 finished! INFO @ Mon, 06 Nov 2017 12:25:38: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:25:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:25:39: #1 tag size is determined as 51 bps INFO @ Mon, 06 Nov 2017 12:25:39: #1 tag size = 51 INFO @ Mon, 06 Nov 2017 12:25:39: #1 total tags in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:39: #1 user defined the maximum tags... INFO @ Mon, 06 Nov 2017 12:25:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Nov 2017 12:25:39: #1 tags after filtering in treatment: 24996435 INFO @ Mon, 06 Nov 2017 12:25:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Nov 2017 12:25:39: #1 finished! INFO @ Mon, 06 Nov 2017 12:25:39: #2 Build Peak Model... INFO @ Mon, 06 Nov 2017 12:25:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Nov 2017 12:25:40: #2 number of paired peaks: 94 WARNING @ Mon, 06 Nov 2017 12:25:40: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:25:40: Process for pairing-model is terminated! cat: SRX2804229.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2804229.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Nov 2017 12:25:41: #2 number of paired peaks: 94 WARNING @ Mon, 06 Nov 2017 12:25:41: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Nov 2017 12:25:41: Process for pairing-model is terminated! cat: SRX2804229.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2804229.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2804229.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。