Job ID = 1294369 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,264,926 reads read : 25,264,926 reads written : 25,264,926 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:58 25264926 reads; of these: 25264926 (100.00%) were unpaired; of these: 2062176 (8.16%) aligned 0 times 17205854 (68.10%) aligned exactly 1 time 5996896 (23.74%) aligned >1 times 91.84% overall alignment rate Time searching: 00:09:58 Overall time: 00:09:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5442159 / 23202750 = 0.2345 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 07:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 07:10:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 07:10:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 07:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 07:10:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 07:10:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 07:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 07:10:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 07:10:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 07:10:42: 1000000 INFO @ Mon, 03 Jun 2019 07:10:43: 1000000 INFO @ Mon, 03 Jun 2019 07:10:44: 1000000 INFO @ Mon, 03 Jun 2019 07:10:49: 2000000 INFO @ Mon, 03 Jun 2019 07:10:51: 2000000 INFO @ Mon, 03 Jun 2019 07:10:53: 2000000 INFO @ Mon, 03 Jun 2019 07:10:57: 3000000 INFO @ Mon, 03 Jun 2019 07:10:59: 3000000 INFO @ Mon, 03 Jun 2019 07:11:03: 3000000 INFO @ Mon, 03 Jun 2019 07:11:04: 4000000 INFO @ Mon, 03 Jun 2019 07:11:08: 4000000 INFO @ Mon, 03 Jun 2019 07:11:12: 5000000 INFO @ Mon, 03 Jun 2019 07:11:13: 4000000 INFO @ Mon, 03 Jun 2019 07:11:16: 5000000 INFO @ Mon, 03 Jun 2019 07:11:20: 6000000 INFO @ Mon, 03 Jun 2019 07:11:23: 5000000 INFO @ Mon, 03 Jun 2019 07:11:25: 6000000 INFO @ Mon, 03 Jun 2019 07:11:27: 7000000 INFO @ Mon, 03 Jun 2019 07:11:33: 6000000 INFO @ Mon, 03 Jun 2019 07:11:33: 7000000 INFO @ Mon, 03 Jun 2019 07:11:34: 8000000 INFO @ Mon, 03 Jun 2019 07:11:41: 8000000 INFO @ Mon, 03 Jun 2019 07:11:41: 9000000 INFO @ Mon, 03 Jun 2019 07:11:42: 7000000 INFO @ Mon, 03 Jun 2019 07:11:49: 10000000 INFO @ Mon, 03 Jun 2019 07:11:49: 9000000 INFO @ Mon, 03 Jun 2019 07:11:52: 8000000 INFO @ Mon, 03 Jun 2019 07:11:56: 11000000 INFO @ Mon, 03 Jun 2019 07:11:57: 10000000 INFO @ Mon, 03 Jun 2019 07:12:01: 9000000 INFO @ Mon, 03 Jun 2019 07:12:03: 12000000 INFO @ Mon, 03 Jun 2019 07:12:05: 11000000 INFO @ Mon, 03 Jun 2019 07:12:10: 13000000 INFO @ Mon, 03 Jun 2019 07:12:11: 10000000 INFO @ Mon, 03 Jun 2019 07:12:13: 12000000 INFO @ Mon, 03 Jun 2019 07:12:18: 14000000 INFO @ Mon, 03 Jun 2019 07:12:20: 11000000 INFO @ Mon, 03 Jun 2019 07:12:21: 13000000 INFO @ Mon, 03 Jun 2019 07:12:25: 15000000 INFO @ Mon, 03 Jun 2019 07:12:29: 12000000 INFO @ Mon, 03 Jun 2019 07:12:29: 14000000 INFO @ Mon, 03 Jun 2019 07:12:32: 16000000 INFO @ Mon, 03 Jun 2019 07:12:37: 15000000 INFO @ Mon, 03 Jun 2019 07:12:38: 13000000 INFO @ Mon, 03 Jun 2019 07:12:39: 17000000 INFO @ Mon, 03 Jun 2019 07:12:45: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 07:12:45: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 07:12:45: #1 total tags in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:12:45: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:12:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:12:45: #1 tags after filtering in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:12:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:12:45: #1 finished! INFO @ Mon, 03 Jun 2019 07:12:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:12:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:12:46: 16000000 INFO @ Mon, 03 Jun 2019 07:12:47: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 07:12:47: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:12:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 07:12:48: 14000000 INFO @ Mon, 03 Jun 2019 07:12:54: 17000000 INFO @ Mon, 03 Jun 2019 07:12:57: 15000000 INFO @ Mon, 03 Jun 2019 07:13:00: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 07:13:00: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 07:13:00: #1 total tags in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:13:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:13:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:13:00: #1 tags after filtering in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:13:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:13:00: #1 finished! INFO @ Mon, 03 Jun 2019 07:13:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:13:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:13:02: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 07:13:02: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:13:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 07:13:07: 16000000 INFO @ Mon, 03 Jun 2019 07:13:16: 17000000 INFO @ Mon, 03 Jun 2019 07:13:23: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 07:13:23: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 07:13:23: #1 total tags in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:13:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:13:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:13:23: #1 tags after filtering in treatment: 17760591 INFO @ Mon, 03 Jun 2019 07:13:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:13:23: #1 finished! INFO @ Mon, 03 Jun 2019 07:13:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:13:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:13:25: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 07:13:25: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:13:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX271403/SRX271403.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。