Job ID = 10845191 sra ファイルのダウンロード中... Completed: 153415K bytes transferred in 13 seconds (92800K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 3555682 spots for /home/okishinya/chipatlas/results/dm3/SRX2642407/SRR5345747.sra Written 3555682 spots for /home/okishinya/chipatlas/results/dm3/SRX2642407/SRR5345747.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:51 3555682 reads; of these: 3555682 (100.00%) were unpaired; of these: 846513 (23.81%) aligned 0 times 2038042 (57.32%) aligned exactly 1 time 671127 (18.87%) aligned >1 times 76.19% overall alignment rate Time searching: 00:01:51 Overall time: 00:01:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 58767 / 2709169 = 0.0217 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 04 Jul 2018 09:29:42: # Command line: callpeak -t SRX2642407.bam -f BAM -g dm -n SRX2642407.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2642407.20 # format = BAM # ChIP-seq file = ['SRX2642407.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:29:42: # Command line: callpeak -t SRX2642407.bam -f BAM -g dm -n SRX2642407.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2642407.05 # format = BAM # ChIP-seq file = ['SRX2642407.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:29:42: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:29:42: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:29:42: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:29:42: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:29:42: # Command line: callpeak -t SRX2642407.bam -f BAM -g dm -n SRX2642407.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2642407.10 # format = BAM # ChIP-seq file = ['SRX2642407.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:29:42: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:29:42: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:29:50: 1000000 INFO @ Wed, 04 Jul 2018 09:29:50: 1000000 INFO @ Wed, 04 Jul 2018 09:29:50: 1000000 INFO @ Wed, 04 Jul 2018 09:29:58: 2000000 INFO @ Wed, 04 Jul 2018 09:29:58: 2000000 INFO @ Wed, 04 Jul 2018 09:29:58: 2000000 INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size is determined as 97 bps INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size = 97 INFO @ Wed, 04 Jul 2018 09:30:03: #1 total tags in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:30:03: #1 tags after filtering in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:30:03: #1 finished! INFO @ Wed, 04 Jul 2018 09:30:03: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size is determined as 97 bps INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size = 97 INFO @ Wed, 04 Jul 2018 09:30:03: #1 total tags in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:30:03: #1 tags after filtering in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:30:03: #1 finished! INFO @ Wed, 04 Jul 2018 09:30:03: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:30:03: #2 number of paired peaks: 170 WARNING @ Wed, 04 Jul 2018 09:30:03: Fewer paired peaks (170) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 170 pairs to build model! INFO @ Wed, 04 Jul 2018 09:30:03: start model_add_line... INFO @ Wed, 04 Jul 2018 09:30:03: start X-correlation... INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size is determined as 97 bps INFO @ Wed, 04 Jul 2018 09:30:03: #1 tag size = 97 INFO @ Wed, 04 Jul 2018 09:30:03: #1 total tags in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:30:03: #1 tags after filtering in treatment: 2650402 INFO @ Wed, 04 Jul 2018 09:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:30:03: #1 finished! INFO @ Wed, 04 Jul 2018 09:30:03: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:30:03: #2 number of paired peaks: 170 WARNING @ Wed, 04 Jul 2018 09:30:03: Fewer paired peaks (170) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 170 pairs to build model! INFO @ Wed, 04 Jul 2018 09:30:03: start model_add_line... INFO @ Wed, 04 Jul 2018 09:30:03: start X-correlation... INFO @ Wed, 04 Jul 2018 09:30:03: end of X-cor INFO @ Wed, 04 Jul 2018 09:30:03: end of X-cor INFO @ Wed, 04 Jul 2018 09:30:03: #2 finished! INFO @ Wed, 04 Jul 2018 09:30:03: #2 finished! INFO @ Wed, 04 Jul 2018 09:30:03: #2 predicted fragment length is 94 bps INFO @ Wed, 04 Jul 2018 09:30:03: #2 predicted fragment length is 94 bps INFO @ Wed, 04 Jul 2018 09:30:03: #2 alternative fragment length(s) may be 94 bps INFO @ Wed, 04 Jul 2018 09:30:03: #2 alternative fragment length(s) may be 94 bps INFO @ Wed, 04 Jul 2018 09:30:03: #2.2 Generate R script for model : SRX2642407.05_model.r INFO @ Wed, 04 Jul 2018 09:30:03: #2.2 Generate R script for model : SRX2642407.20_model.r WARNING @ Wed, 04 Jul 2018 09:30:03: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:30:03: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Wed, 04 Jul 2018 09:30:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:30:03: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:30:03: #3 Pre-compute pvalue-qvalue table... WARNING @ Wed, 04 Jul 2018 09:30:03: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:30:03: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Wed, 04 Jul 2018 09:30:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:30:03: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:30:03: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 04 Jul 2018 09:30:04: #2 number of paired peaks: 170 WARNING @ Wed, 04 Jul 2018 09:30:04: Fewer paired peaks (170) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 170 pairs to build model! INFO @ Wed, 04 Jul 2018 09:30:04: start model_add_line... INFO @ Wed, 04 Jul 2018 09:30:04: start X-correlation... INFO @ Wed, 04 Jul 2018 09:30:04: end of X-cor INFO @ Wed, 04 Jul 2018 09:30:04: #2 finished! INFO @ Wed, 04 Jul 2018 09:30:04: #2 predicted fragment length is 94 bps INFO @ Wed, 04 Jul 2018 09:30:04: #2 alternative fragment length(s) may be 94 bps INFO @ Wed, 04 Jul 2018 09:30:04: #2.2 Generate R script for model : SRX2642407.10_model.r WARNING @ Wed, 04 Jul 2018 09:30:04: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:30:04: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Wed, 04 Jul 2018 09:30:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:30:04: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:30:04: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 04 Jul 2018 09:30:09: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:30:10: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:30:10: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write output xls file... SRX2642407.05_peaks.xls INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write peak in narrowPeak format file... SRX2642407.05_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write summits bed file... SRX2642407.05_summits.bed INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write output xls file... SRX2642407.10_peaks.xls INFO @ Wed, 04 Jul 2018 09:30:13: Done! INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write peak in narrowPeak format file... SRX2642407.10_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write summits bed file... SRX2642407.10_summits.bed INFO @ Wed, 04 Jul 2018 09:30:13: Done! pass1 - making usageList (4 chroms): 1 millis pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (261 records, 4 fields): 1 millis pass2 - checking and writing primary data (390 records, 4 fields): 2 millis CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write output xls file... SRX2642407.20_peaks.xls INFO @ Wed, 04 Jul 2018 09:30:13: #4 Write peak in narrowPeak format file... SRX2642407.20_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:30:14: #4 Write summits bed file... SRX2642407.20_summits.bed INFO @ Wed, 04 Jul 2018 09:30:14: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (141 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。