Job ID = 11171235 sra ファイルのダウンロード中... Completed: 536922K bytes transferred in 21 seconds (201525K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 27915269 spots for /home/okishinya/chipatlas/results/dm3/SRX2618554/SRR5319108.sra Written 27915269 spots for /home/okishinya/chipatlas/results/dm3/SRX2618554/SRR5319108.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:31 27915269 reads; of these: 27915269 (100.00%) were unpaired; of these: 876758 (3.14%) aligned 0 times 19734617 (70.69%) aligned exactly 1 time 7303894 (26.16%) aligned >1 times 96.86% overall alignment rate Time searching: 00:11:31 Overall time: 00:11:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3041579 / 27038511 = 0.1125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:29:33: # Command line: callpeak -t SRX2618554.bam -f BAM -g dm -n SRX2618554.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618554.10 # format = BAM # ChIP-seq file = ['SRX2618554.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:33: # Command line: callpeak -t SRX2618554.bam -f BAM -g dm -n SRX2618554.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618554.05 # format = BAM # ChIP-seq file = ['SRX2618554.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:33: # Command line: callpeak -t SRX2618554.bam -f BAM -g dm -n SRX2618554.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618554.20 # format = BAM # ChIP-seq file = ['SRX2618554.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:39: 1000000 INFO @ Sat, 08 Sep 2018 13:29:39: 1000000 INFO @ Sat, 08 Sep 2018 13:29:39: 1000000 INFO @ Sat, 08 Sep 2018 13:29:46: 2000000 INFO @ Sat, 08 Sep 2018 13:29:46: 2000000 INFO @ Sat, 08 Sep 2018 13:29:46: 2000000 INFO @ Sat, 08 Sep 2018 13:29:53: 3000000 INFO @ Sat, 08 Sep 2018 13:29:53: 3000000 INFO @ Sat, 08 Sep 2018 13:29:53: 3000000 INFO @ Sat, 08 Sep 2018 13:29:59: 4000000 INFO @ Sat, 08 Sep 2018 13:29:59: 4000000 INFO @ Sat, 08 Sep 2018 13:30:00: 4000000 INFO @ Sat, 08 Sep 2018 13:30:06: 5000000 INFO @ Sat, 08 Sep 2018 13:30:06: 5000000 INFO @ Sat, 08 Sep 2018 13:30:06: 5000000 INFO @ Sat, 08 Sep 2018 13:30:12: 6000000 INFO @ Sat, 08 Sep 2018 13:30:13: 6000000 INFO @ Sat, 08 Sep 2018 13:30:13: 6000000 INFO @ Sat, 08 Sep 2018 13:30:19: 7000000 INFO @ Sat, 08 Sep 2018 13:30:19: 7000000 INFO @ Sat, 08 Sep 2018 13:30:19: 7000000 INFO @ Sat, 08 Sep 2018 13:30:25: 8000000 INFO @ Sat, 08 Sep 2018 13:30:26: 8000000 INFO @ Sat, 08 Sep 2018 13:30:26: 8000000 INFO @ Sat, 08 Sep 2018 13:30:32: 9000000 INFO @ Sat, 08 Sep 2018 13:30:32: 9000000 INFO @ Sat, 08 Sep 2018 13:30:33: 9000000 INFO @ Sat, 08 Sep 2018 13:30:38: 10000000 INFO @ Sat, 08 Sep 2018 13:30:39: 10000000 INFO @ Sat, 08 Sep 2018 13:30:39: 10000000 INFO @ Sat, 08 Sep 2018 13:30:45: 11000000 INFO @ Sat, 08 Sep 2018 13:30:45: 11000000 INFO @ Sat, 08 Sep 2018 13:30:46: 11000000 INFO @ Sat, 08 Sep 2018 13:30:51: 12000000 INFO @ Sat, 08 Sep 2018 13:30:52: 12000000 INFO @ Sat, 08 Sep 2018 13:30:52: 12000000 INFO @ Sat, 08 Sep 2018 13:30:58: 13000000 INFO @ Sat, 08 Sep 2018 13:30:59: 13000000 INFO @ Sat, 08 Sep 2018 13:30:59: 13000000 INFO @ Sat, 08 Sep 2018 13:31:04: 14000000 INFO @ Sat, 08 Sep 2018 13:31:05: 14000000 INFO @ Sat, 08 Sep 2018 13:31:05: 14000000 INFO @ Sat, 08 Sep 2018 13:31:11: 15000000 INFO @ Sat, 08 Sep 2018 13:31:12: 15000000 INFO @ Sat, 08 Sep 2018 13:31:12: 15000000 INFO @ Sat, 08 Sep 2018 13:31:17: 16000000 INFO @ Sat, 08 Sep 2018 13:31:18: 16000000 INFO @ Sat, 08 Sep 2018 13:31:19: 16000000 INFO @ Sat, 08 Sep 2018 13:31:24: 17000000 INFO @ Sat, 08 Sep 2018 13:31:25: 17000000 INFO @ Sat, 08 Sep 2018 13:31:25: 17000000 INFO @ Sat, 08 Sep 2018 13:31:30: 18000000 INFO @ Sat, 08 Sep 2018 13:31:31: 18000000 INFO @ Sat, 08 Sep 2018 13:31:32: 18000000 INFO @ Sat, 08 Sep 2018 13:31:37: 19000000 INFO @ Sat, 08 Sep 2018 13:31:38: 19000000 INFO @ Sat, 08 Sep 2018 13:31:38: 19000000 INFO @ Sat, 08 Sep 2018 13:31:43: 20000000 INFO @ Sat, 08 Sep 2018 13:31:45: 20000000 INFO @ Sat, 08 Sep 2018 13:31:45: 20000000 INFO @ Sat, 08 Sep 2018 13:31:50: 21000000 INFO @ Sat, 08 Sep 2018 13:31:51: 21000000 INFO @ Sat, 08 Sep 2018 13:31:52: 21000000 INFO @ Sat, 08 Sep 2018 13:31:56: 22000000 INFO @ Sat, 08 Sep 2018 13:31:58: 22000000 INFO @ Sat, 08 Sep 2018 13:31:59: 22000000 INFO @ Sat, 08 Sep 2018 13:32:03: 23000000 INFO @ Sat, 08 Sep 2018 13:32:04: 23000000 INFO @ Sat, 08 Sep 2018 13:32:05: 23000000 INFO @ Sat, 08 Sep 2018 13:32:10: #1 tag size is determined as 51 bps INFO @ Sat, 08 Sep 2018 13:32:10: #1 tag size = 51 INFO @ Sat, 08 Sep 2018 13:32:10: #1 total tags in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:11: #1 tags after filtering in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:11: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size is determined as 51 bps INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size = 51 INFO @ Sat, 08 Sep 2018 13:32:11: #1 total tags in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:11: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:11: #1 tags after filtering in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:11: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:12: #1 tag size is determined as 51 bps INFO @ Sat, 08 Sep 2018 13:32:12: #1 tag size = 51 INFO @ Sat, 08 Sep 2018 13:32:12: #1 total tags in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:12: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:12: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:12: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:12: Process for pairing-model is terminated! cat: SRX2618554.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618554.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:32:13: #1 tags after filtering in treatment: 23996932 INFO @ Sat, 08 Sep 2018 13:32:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:13: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:13: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:13: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:13: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:13: Process for pairing-model is terminated! cat: SRX2618554.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618554.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:32:14: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:14: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:14: Process for pairing-model is terminated! cat: SRX2618554.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618554.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618554.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。