Job ID = 11171229 sra ファイルのダウンロード中... Completed: 433252K bytes transferred in 22 seconds (156039K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 22628889 spots for /home/okishinya/chipatlas/results/dm3/SRX2618548/SRR5319102.sra Written 22628889 spots for /home/okishinya/chipatlas/results/dm3/SRX2618548/SRR5319102.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:45 22628889 reads; of these: 22628889 (100.00%) were unpaired; of these: 815082 (3.60%) aligned 0 times 14255168 (63.00%) aligned exactly 1 time 7558639 (33.40%) aligned >1 times 96.40% overall alignment rate Time searching: 00:10:45 Overall time: 00:10:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2663289 / 21813807 = 0.1221 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:23:39: # Command line: callpeak -t SRX2618548.bam -f BAM -g dm -n SRX2618548.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618548.05 # format = BAM # ChIP-seq file = ['SRX2618548.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:23:39: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:23:39: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:23:39: # Command line: callpeak -t SRX2618548.bam -f BAM -g dm -n SRX2618548.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618548.20 # format = BAM # ChIP-seq file = ['SRX2618548.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:23:39: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:23:39: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:23:39: # Command line: callpeak -t SRX2618548.bam -f BAM -g dm -n SRX2618548.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618548.10 # format = BAM # ChIP-seq file = ['SRX2618548.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:23:39: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:23:39: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:23:44: 1000000 INFO @ Sat, 08 Sep 2018 13:23:44: 1000000 INFO @ Sat, 08 Sep 2018 13:23:44: 1000000 INFO @ Sat, 08 Sep 2018 13:23:50: 2000000 INFO @ Sat, 08 Sep 2018 13:23:50: 2000000 INFO @ Sat, 08 Sep 2018 13:23:50: 2000000 INFO @ Sat, 08 Sep 2018 13:23:56: 3000000 INFO @ Sat, 08 Sep 2018 13:23:56: 3000000 INFO @ Sat, 08 Sep 2018 13:23:56: 3000000 INFO @ Sat, 08 Sep 2018 13:24:02: 4000000 INFO @ Sat, 08 Sep 2018 13:24:02: 4000000 INFO @ Sat, 08 Sep 2018 13:24:02: 4000000 INFO @ Sat, 08 Sep 2018 13:24:08: 5000000 INFO @ Sat, 08 Sep 2018 13:24:08: 5000000 INFO @ Sat, 08 Sep 2018 13:24:08: 5000000 INFO @ Sat, 08 Sep 2018 13:24:14: 6000000 INFO @ Sat, 08 Sep 2018 13:24:14: 6000000 INFO @ Sat, 08 Sep 2018 13:24:15: 6000000 INFO @ Sat, 08 Sep 2018 13:24:20: 7000000 INFO @ Sat, 08 Sep 2018 13:24:20: 7000000 INFO @ Sat, 08 Sep 2018 13:24:21: 7000000 INFO @ Sat, 08 Sep 2018 13:24:25: 8000000 INFO @ Sat, 08 Sep 2018 13:24:25: 8000000 INFO @ Sat, 08 Sep 2018 13:24:27: 8000000 INFO @ Sat, 08 Sep 2018 13:24:31: 9000000 INFO @ Sat, 08 Sep 2018 13:24:31: 9000000 INFO @ Sat, 08 Sep 2018 13:24:33: 9000000 INFO @ Sat, 08 Sep 2018 13:24:37: 10000000 INFO @ Sat, 08 Sep 2018 13:24:37: 10000000 INFO @ Sat, 08 Sep 2018 13:24:39: 10000000 INFO @ Sat, 08 Sep 2018 13:24:43: 11000000 INFO @ Sat, 08 Sep 2018 13:24:43: 11000000 INFO @ Sat, 08 Sep 2018 13:24:45: 11000000 INFO @ Sat, 08 Sep 2018 13:24:49: 12000000 INFO @ Sat, 08 Sep 2018 13:24:49: 12000000 INFO @ Sat, 08 Sep 2018 13:24:51: 12000000 INFO @ Sat, 08 Sep 2018 13:24:55: 13000000 INFO @ Sat, 08 Sep 2018 13:24:55: 13000000 INFO @ Sat, 08 Sep 2018 13:24:57: 13000000 INFO @ Sat, 08 Sep 2018 13:25:01: 14000000 INFO @ Sat, 08 Sep 2018 13:25:02: 14000000 INFO @ Sat, 08 Sep 2018 13:25:04: 14000000 INFO @ Sat, 08 Sep 2018 13:25:07: 15000000 INFO @ Sat, 08 Sep 2018 13:25:09: 15000000 INFO @ Sat, 08 Sep 2018 13:25:10: 15000000 INFO @ Sat, 08 Sep 2018 13:25:14: 16000000 INFO @ Sat, 08 Sep 2018 13:25:16: 16000000 INFO @ Sat, 08 Sep 2018 13:25:17: 16000000 INFO @ Sat, 08 Sep 2018 13:25:20: 17000000 INFO @ Sat, 08 Sep 2018 13:25:23: 17000000 INFO @ Sat, 08 Sep 2018 13:25:24: 17000000 INFO @ Sat, 08 Sep 2018 13:25:26: 18000000 INFO @ Sat, 08 Sep 2018 13:25:30: 18000000 INFO @ Sat, 08 Sep 2018 13:25:30: 18000000 INFO @ Sat, 08 Sep 2018 13:25:33: 19000000 INFO @ Sat, 08 Sep 2018 13:25:34: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:25:34: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:25:34: #1 total tags in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:34: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:25:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:25:34: #1 tags after filtering in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:25:34: #1 finished! INFO @ Sat, 08 Sep 2018 13:25:34: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:25:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:25:35: #2 number of paired peaks: 76 WARNING @ Sat, 08 Sep 2018 13:25:35: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:25:35: Process for pairing-model is terminated! cat: SRX2618548.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618548.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:25:36: 19000000 INFO @ Sat, 08 Sep 2018 13:25:36: 19000000 INFO @ Sat, 08 Sep 2018 13:25:37: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:25:37: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:25:37: #1 total tags in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:37: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:25:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:25:37: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:25:37: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:25:37: #1 total tags in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:37: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:25:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:25:37: #1 tags after filtering in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:25:37: #1 finished! INFO @ Sat, 08 Sep 2018 13:25:37: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:25:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:25:38: #1 tags after filtering in treatment: 19150518 INFO @ Sat, 08 Sep 2018 13:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:25:38: #1 finished! INFO @ Sat, 08 Sep 2018 13:25:38: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:25:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:25:39: #2 number of paired peaks: 76 WARNING @ Sat, 08 Sep 2018 13:25:39: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:25:39: Process for pairing-model is terminated! cat: SRX2618548.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618548.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:25:39: #2 number of paired peaks: 76 WARNING @ Sat, 08 Sep 2018 13:25:39: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:25:39: Process for pairing-model is terminated! cat: SRX2618548.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618548.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618548.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。