Job ID = 11171225 sra ファイルのダウンロード中... Completed: 478793K bytes transferred in 23 seconds (168070K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 19539289 spots for /home/okishinya/chipatlas/results/dm3/SRX2618545/SRR5319099.sra Written 19539289 spots for /home/okishinya/chipatlas/results/dm3/SRX2618545/SRR5319099.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:08:26 19539289 reads; of these: 19539289 (100.00%) were unpaired; of these: 1800662 (9.22%) aligned 0 times 11676786 (59.76%) aligned exactly 1 time 6061841 (31.02%) aligned >1 times 90.78% overall alignment rate Time searching: 00:08:27 Overall time: 00:08:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1565485 / 17738627 = 0.0883 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:18:45: # Command line: callpeak -t SRX2618545.bam -f BAM -g dm -n SRX2618545.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618545.05 # format = BAM # ChIP-seq file = ['SRX2618545.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:45: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:45: # Command line: callpeak -t SRX2618545.bam -f BAM -g dm -n SRX2618545.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618545.20 # format = BAM # ChIP-seq file = ['SRX2618545.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:45: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:45: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:45: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:45: # Command line: callpeak -t SRX2618545.bam -f BAM -g dm -n SRX2618545.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618545.10 # format = BAM # ChIP-seq file = ['SRX2618545.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:45: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:45: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:52: 1000000 INFO @ Sat, 08 Sep 2018 13:18:52: 1000000 INFO @ Sat, 08 Sep 2018 13:18:52: 1000000 INFO @ Sat, 08 Sep 2018 13:18:58: 2000000 INFO @ Sat, 08 Sep 2018 13:18:58: 2000000 INFO @ Sat, 08 Sep 2018 13:18:58: 2000000 INFO @ Sat, 08 Sep 2018 13:19:04: 3000000 INFO @ Sat, 08 Sep 2018 13:19:04: 3000000 INFO @ Sat, 08 Sep 2018 13:19:05: 3000000 INFO @ Sat, 08 Sep 2018 13:19:10: 4000000 INFO @ Sat, 08 Sep 2018 13:19:11: 4000000 INFO @ Sat, 08 Sep 2018 13:19:11: 4000000 INFO @ Sat, 08 Sep 2018 13:19:17: 5000000 INFO @ Sat, 08 Sep 2018 13:19:17: 5000000 INFO @ Sat, 08 Sep 2018 13:19:18: 5000000 INFO @ Sat, 08 Sep 2018 13:19:23: 6000000 INFO @ Sat, 08 Sep 2018 13:19:23: 6000000 INFO @ Sat, 08 Sep 2018 13:19:24: 6000000 INFO @ Sat, 08 Sep 2018 13:19:29: 7000000 INFO @ Sat, 08 Sep 2018 13:19:30: 7000000 INFO @ Sat, 08 Sep 2018 13:19:31: 7000000 INFO @ Sat, 08 Sep 2018 13:19:36: 8000000 INFO @ Sat, 08 Sep 2018 13:19:36: 8000000 INFO @ Sat, 08 Sep 2018 13:19:37: 8000000 INFO @ Sat, 08 Sep 2018 13:19:42: 9000000 INFO @ Sat, 08 Sep 2018 13:19:43: 9000000 INFO @ Sat, 08 Sep 2018 13:19:44: 9000000 INFO @ Sat, 08 Sep 2018 13:19:48: 10000000 INFO @ Sat, 08 Sep 2018 13:19:50: 10000000 INFO @ Sat, 08 Sep 2018 13:19:50: 10000000 INFO @ Sat, 08 Sep 2018 13:19:55: 11000000 INFO @ Sat, 08 Sep 2018 13:19:56: 11000000 INFO @ Sat, 08 Sep 2018 13:19:57: 11000000 INFO @ Sat, 08 Sep 2018 13:20:01: 12000000 INFO @ Sat, 08 Sep 2018 13:20:03: 12000000 INFO @ Sat, 08 Sep 2018 13:20:03: 12000000 INFO @ Sat, 08 Sep 2018 13:20:07: 13000000 INFO @ Sat, 08 Sep 2018 13:20:09: 13000000 INFO @ Sat, 08 Sep 2018 13:20:10: 13000000 INFO @ Sat, 08 Sep 2018 13:20:14: 14000000 INFO @ Sat, 08 Sep 2018 13:20:15: 14000000 INFO @ Sat, 08 Sep 2018 13:20:16: 14000000 INFO @ Sat, 08 Sep 2018 13:20:20: 15000000 INFO @ Sat, 08 Sep 2018 13:20:22: 15000000 INFO @ Sat, 08 Sep 2018 13:20:23: 15000000 INFO @ Sat, 08 Sep 2018 13:20:26: 16000000 INFO @ Sat, 08 Sep 2018 13:20:28: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:28: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:28: #1 total tags in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:28: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:28: #1 tags after filtering in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:28: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:28: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:28: 16000000 INFO @ Sat, 08 Sep 2018 13:20:29: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:29: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:29: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 13:20:29: 16000000 cat: SRX2618545.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618545.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:29: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:29: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:29: #1 total tags in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:29: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:30: #1 tags after filtering in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:30: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:30: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:31: #1 total tags in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:31: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:31: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:31: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:31: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 13:20:31: #1 tags after filtering in treatment: 16173142 INFO @ Sat, 08 Sep 2018 13:20:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:31: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:31: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:31: #2 looking for paired plus/minus strand peaks... cat: SRX2618545.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618545.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:32: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:32: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:32: Process for pairing-model is terminated! cat: SRX2618545.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618545.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618545.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。