Job ID = 11171203 sra ファイルのダウンロード中... Completed: 897983K bytes transferred in 39 seconds (186396K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 46509756 spots for /home/okishinya/chipatlas/results/dm3/SRX2618513/SRR5319067.sra Written 46509756 spots for /home/okishinya/chipatlas/results/dm3/SRX2618513/SRR5319067.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:48 46509756 reads; of these: 46509756 (100.00%) were unpaired; of these: 17238973 (37.07%) aligned 0 times 25405974 (54.63%) aligned exactly 1 time 3864809 (8.31%) aligned >1 times 62.93% overall alignment rate Time searching: 00:10:48 Overall time: 00:10:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3300437 / 29270783 = 0.1128 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:12:41: # Command line: callpeak -t SRX2618513.bam -f BAM -g dm -n SRX2618513.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618513.05 # format = BAM # ChIP-seq file = ['SRX2618513.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:12:41: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:12:41: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:12:41: # Command line: callpeak -t SRX2618513.bam -f BAM -g dm -n SRX2618513.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618513.10 # format = BAM # ChIP-seq file = ['SRX2618513.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:12:41: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:12:41: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:12:41: # Command line: callpeak -t SRX2618513.bam -f BAM -g dm -n SRX2618513.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618513.20 # format = BAM # ChIP-seq file = ['SRX2618513.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:12:41: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:12:41: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:12:48: 1000000 INFO @ Sat, 08 Sep 2018 13:12:48: 1000000 INFO @ Sat, 08 Sep 2018 13:12:48: 1000000 INFO @ Sat, 08 Sep 2018 13:12:54: 2000000 INFO @ Sat, 08 Sep 2018 13:12:54: 2000000 INFO @ Sat, 08 Sep 2018 13:12:55: 2000000 INFO @ Sat, 08 Sep 2018 13:13:01: 3000000 INFO @ Sat, 08 Sep 2018 13:13:01: 3000000 INFO @ Sat, 08 Sep 2018 13:13:03: 3000000 INFO @ Sat, 08 Sep 2018 13:13:08: 4000000 INFO @ Sat, 08 Sep 2018 13:13:08: 4000000 INFO @ Sat, 08 Sep 2018 13:13:11: 4000000 INFO @ Sat, 08 Sep 2018 13:13:15: 5000000 INFO @ Sat, 08 Sep 2018 13:13:15: 5000000 INFO @ Sat, 08 Sep 2018 13:13:18: 5000000 INFO @ Sat, 08 Sep 2018 13:13:22: 6000000 INFO @ Sat, 08 Sep 2018 13:13:22: 6000000 INFO @ Sat, 08 Sep 2018 13:13:26: 6000000 INFO @ Sat, 08 Sep 2018 13:13:29: 7000000 INFO @ Sat, 08 Sep 2018 13:13:29: 7000000 INFO @ Sat, 08 Sep 2018 13:13:34: 7000000 INFO @ Sat, 08 Sep 2018 13:13:36: 8000000 INFO @ Sat, 08 Sep 2018 13:13:36: 8000000 INFO @ Sat, 08 Sep 2018 13:13:41: 8000000 INFO @ Sat, 08 Sep 2018 13:13:43: 9000000 INFO @ Sat, 08 Sep 2018 13:13:43: 9000000 INFO @ Sat, 08 Sep 2018 13:13:49: 9000000 INFO @ Sat, 08 Sep 2018 13:13:50: 10000000 INFO @ Sat, 08 Sep 2018 13:13:50: 10000000 INFO @ Sat, 08 Sep 2018 13:13:57: 10000000 INFO @ Sat, 08 Sep 2018 13:13:57: 11000000 INFO @ Sat, 08 Sep 2018 13:13:58: 11000000 INFO @ Sat, 08 Sep 2018 13:14:04: 12000000 INFO @ Sat, 08 Sep 2018 13:14:05: 11000000 INFO @ Sat, 08 Sep 2018 13:14:05: 12000000 INFO @ Sat, 08 Sep 2018 13:14:11: 13000000 INFO @ Sat, 08 Sep 2018 13:14:12: 13000000 INFO @ Sat, 08 Sep 2018 13:14:12: 12000000 INFO @ Sat, 08 Sep 2018 13:14:18: 14000000 INFO @ Sat, 08 Sep 2018 13:14:19: 14000000 INFO @ Sat, 08 Sep 2018 13:14:20: 13000000 INFO @ Sat, 08 Sep 2018 13:14:25: 15000000 INFO @ Sat, 08 Sep 2018 13:14:26: 15000000 INFO @ Sat, 08 Sep 2018 13:14:28: 14000000 INFO @ Sat, 08 Sep 2018 13:14:33: 16000000 INFO @ Sat, 08 Sep 2018 13:14:33: 16000000 INFO @ Sat, 08 Sep 2018 13:14:36: 15000000 INFO @ Sat, 08 Sep 2018 13:14:40: 17000000 INFO @ Sat, 08 Sep 2018 13:14:40: 17000000 INFO @ Sat, 08 Sep 2018 13:14:44: 16000000 INFO @ Sat, 08 Sep 2018 13:14:47: 18000000 INFO @ Sat, 08 Sep 2018 13:14:48: 18000000 INFO @ Sat, 08 Sep 2018 13:14:52: 17000000 INFO @ Sat, 08 Sep 2018 13:14:54: 19000000 INFO @ Sat, 08 Sep 2018 13:14:55: 19000000 INFO @ Sat, 08 Sep 2018 13:15:00: 18000000 INFO @ Sat, 08 Sep 2018 13:15:01: 20000000 INFO @ Sat, 08 Sep 2018 13:15:02: 20000000 INFO @ Sat, 08 Sep 2018 13:15:07: 19000000 INFO @ Sat, 08 Sep 2018 13:15:09: 21000000 INFO @ Sat, 08 Sep 2018 13:15:10: 21000000 INFO @ Sat, 08 Sep 2018 13:15:15: 20000000 INFO @ Sat, 08 Sep 2018 13:15:16: 22000000 INFO @ Sat, 08 Sep 2018 13:15:17: 22000000 INFO @ Sat, 08 Sep 2018 13:15:23: 23000000 INFO @ Sat, 08 Sep 2018 13:15:24: 21000000 INFO @ Sat, 08 Sep 2018 13:15:25: 23000000 INFO @ Sat, 08 Sep 2018 13:15:31: 24000000 INFO @ Sat, 08 Sep 2018 13:15:32: 22000000 INFO @ Sat, 08 Sep 2018 13:15:32: 24000000 INFO @ Sat, 08 Sep 2018 13:15:38: 25000000 INFO @ Sat, 08 Sep 2018 13:15:40: 23000000 INFO @ Sat, 08 Sep 2018 13:15:40: 25000000 INFO @ Sat, 08 Sep 2018 13:15:45: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:15:45: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:15:45: #1 total tags in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:15:45: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:15:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:15:46: #1 tags after filtering in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:15:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:15:46: #1 finished! INFO @ Sat, 08 Sep 2018 13:15:46: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:15:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:15:47: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:15:47: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:15:47: #1 total tags in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:15:47: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:15:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:15:47: 24000000 INFO @ Sat, 08 Sep 2018 13:15:48: #1 tags after filtering in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:15:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:15:48: #1 finished! INFO @ Sat, 08 Sep 2018 13:15:48: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:15:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:15:48: #2 number of paired peaks: 48 WARNING @ Sat, 08 Sep 2018 13:15:48: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:15:48: Process for pairing-model is terminated! cat: SRX2618513.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618513.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:15:49: #2 number of paired peaks: 48 WARNING @ Sat, 08 Sep 2018 13:15:49: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:15:49: Process for pairing-model is terminated! cat: SRX2618513.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618513.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:15:53: 25000000 INFO @ Sat, 08 Sep 2018 13:16:00: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:16:00: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:16:00: #1 total tags in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:16:00: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:16:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:16:00: #1 tags after filtering in treatment: 25970346 INFO @ Sat, 08 Sep 2018 13:16:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:16:00: #1 finished! INFO @ Sat, 08 Sep 2018 13:16:00: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:16:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:16:02: #2 number of paired peaks: 48 WARNING @ Sat, 08 Sep 2018 13:16:02: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:16:02: Process for pairing-model is terminated! cat: SRX2618513.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618513.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618513.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。