Job ID = 6527749 SRX = SRX2618507 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:35:37 prefetch.2.10.7: 1) Downloading 'SRR5319061'... 2020-06-29T13:35:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:38:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:38:57 prefetch.2.10.7: 1) 'SRR5319061' was downloaded successfully 2020-06-29T13:38:57 prefetch.2.10.7: 'SRR5319061' has 0 unresolved dependencies Read 44145239 spots for SRR5319061/SRR5319061.sra Written 44145239 spots for SRR5319061/SRR5319061.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:47 44145239 reads; of these: 44145239 (100.00%) were unpaired; of these: 17226288 (39.02%) aligned 0 times 24339273 (55.13%) aligned exactly 1 time 2579678 (5.84%) aligned >1 times 60.98% overall alignment rate Time searching: 00:07:47 Overall time: 00:07:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3330686 / 26918951 = 0.1237 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:04:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:04:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:04:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:04:40: 1000000 INFO @ Mon, 29 Jun 2020 23:04:46: 2000000 INFO @ Mon, 29 Jun 2020 23:04:51: 3000000 INFO @ Mon, 29 Jun 2020 23:04:56: 4000000 INFO @ Mon, 29 Jun 2020 23:05:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:06: 6000000 INFO @ Mon, 29 Jun 2020 23:05:10: 1000000 INFO @ Mon, 29 Jun 2020 23:05:12: 7000000 INFO @ Mon, 29 Jun 2020 23:05:16: 2000000 INFO @ Mon, 29 Jun 2020 23:05:17: 8000000 INFO @ Mon, 29 Jun 2020 23:05:21: 3000000 INFO @ Mon, 29 Jun 2020 23:05:22: 9000000 INFO @ Mon, 29 Jun 2020 23:05:26: 4000000 INFO @ Mon, 29 Jun 2020 23:05:27: 10000000 INFO @ Mon, 29 Jun 2020 23:05:31: 5000000 INFO @ Mon, 29 Jun 2020 23:05:33: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:37: 6000000 INFO @ Mon, 29 Jun 2020 23:05:38: 12000000 INFO @ Mon, 29 Jun 2020 23:05:40: 1000000 INFO @ Mon, 29 Jun 2020 23:05:42: 7000000 INFO @ Mon, 29 Jun 2020 23:05:43: 13000000 INFO @ Mon, 29 Jun 2020 23:05:46: 2000000 INFO @ Mon, 29 Jun 2020 23:05:47: 8000000 INFO @ Mon, 29 Jun 2020 23:05:49: 14000000 INFO @ Mon, 29 Jun 2020 23:05:51: 3000000 INFO @ Mon, 29 Jun 2020 23:05:53: 9000000 INFO @ Mon, 29 Jun 2020 23:05:54: 15000000 INFO @ Mon, 29 Jun 2020 23:05:56: 4000000 INFO @ Mon, 29 Jun 2020 23:05:58: 10000000 INFO @ Mon, 29 Jun 2020 23:06:00: 16000000 INFO @ Mon, 29 Jun 2020 23:06:02: 5000000 INFO @ Mon, 29 Jun 2020 23:06:03: 11000000 INFO @ Mon, 29 Jun 2020 23:06:05: 17000000 INFO @ Mon, 29 Jun 2020 23:06:07: 6000000 INFO @ Mon, 29 Jun 2020 23:06:08: 12000000 INFO @ Mon, 29 Jun 2020 23:06:10: 18000000 INFO @ Mon, 29 Jun 2020 23:06:12: 7000000 INFO @ Mon, 29 Jun 2020 23:06:13: 13000000 INFO @ Mon, 29 Jun 2020 23:06:16: 19000000 INFO @ Mon, 29 Jun 2020 23:06:18: 8000000 INFO @ Mon, 29 Jun 2020 23:06:19: 14000000 INFO @ Mon, 29 Jun 2020 23:06:21: 20000000 INFO @ Mon, 29 Jun 2020 23:06:23: 9000000 INFO @ Mon, 29 Jun 2020 23:06:24: 15000000 INFO @ Mon, 29 Jun 2020 23:06:26: 21000000 INFO @ Mon, 29 Jun 2020 23:06:28: 10000000 INFO @ Mon, 29 Jun 2020 23:06:30: 16000000 INFO @ Mon, 29 Jun 2020 23:06:31: 22000000 INFO @ Mon, 29 Jun 2020 23:06:33: 11000000 INFO @ Mon, 29 Jun 2020 23:06:35: 17000000 INFO @ Mon, 29 Jun 2020 23:06:36: 23000000 INFO @ Mon, 29 Jun 2020 23:06:39: 12000000 INFO @ Mon, 29 Jun 2020 23:06:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:06:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:06:39: #1 total tags in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:06:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:40: #1 tags after filtering in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:06:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:40: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:40: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:40: 18000000 INFO @ Mon, 29 Jun 2020 23:06:41: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:06:41: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:06:44: 13000000 INFO @ Mon, 29 Jun 2020 23:06:46: 19000000 INFO @ Mon, 29 Jun 2020 23:06:49: 14000000 INFO @ Mon, 29 Jun 2020 23:06:51: 20000000 INFO @ Mon, 29 Jun 2020 23:06:55: 15000000 INFO @ Mon, 29 Jun 2020 23:06:56: 21000000 INFO @ Mon, 29 Jun 2020 23:07:00: 16000000 INFO @ Mon, 29 Jun 2020 23:07:01: 22000000 INFO @ Mon, 29 Jun 2020 23:07:05: 17000000 INFO @ Mon, 29 Jun 2020 23:07:06: 23000000 INFO @ Mon, 29 Jun 2020 23:07:09: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:09: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:09: #1 total tags in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:07:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:09: #1 tags after filtering in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:09: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:11: 18000000 INFO @ Mon, 29 Jun 2020 23:07:11: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:07:11: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:07:16: 19000000 INFO @ Mon, 29 Jun 2020 23:07:21: 20000000 INFO @ Mon, 29 Jun 2020 23:07:27: 21000000 INFO @ Mon, 29 Jun 2020 23:07:31: 22000000 INFO @ Mon, 29 Jun 2020 23:07:36: 23000000 INFO @ Mon, 29 Jun 2020 23:07:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:39: #1 total tags in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:07:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:40: #1 tags after filtering in treatment: 23588265 INFO @ Mon, 29 Jun 2020 23:07:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:40: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:40: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:41: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:07:41: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618507/SRX2618507.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。