Job ID = 6527746 SRX = SRX2618502 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:18:20 prefetch.2.10.7: 1) Downloading 'SRR5319056'... 2020-06-29T13:18:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:20:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:20:52 prefetch.2.10.7: 'SRR5319056' is valid 2020-06-29T13:20:52 prefetch.2.10.7: 1) 'SRR5319056' was downloaded successfully 2020-06-29T13:20:52 prefetch.2.10.7: 'SRR5319056' has 0 unresolved dependencies Read 20795653 spots for SRR5319056/SRR5319056.sra Written 20795653 spots for SRR5319056/SRR5319056.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:33 20795653 reads; of these: 20795653 (100.00%) were unpaired; of these: 6357984 (30.57%) aligned 0 times 12441674 (59.83%) aligned exactly 1 time 1995995 (9.60%) aligned >1 times 69.43% overall alignment rate Time searching: 00:04:33 Overall time: 00:04:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1221068 / 14437669 = 0.0846 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:37:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:37:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:37:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:37:53: 1000000 INFO @ Mon, 29 Jun 2020 22:37:59: 2000000 INFO @ Mon, 29 Jun 2020 22:38:05: 3000000 INFO @ Mon, 29 Jun 2020 22:38:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:38:16: 5000000 INFO @ Mon, 29 Jun 2020 22:38:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:38:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:38:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:38:22: 6000000 INFO @ Mon, 29 Jun 2020 22:38:24: 1000000 INFO @ Mon, 29 Jun 2020 22:38:28: 7000000 INFO @ Mon, 29 Jun 2020 22:38:30: 2000000 INFO @ Mon, 29 Jun 2020 22:38:35: 8000000 INFO @ Mon, 29 Jun 2020 22:38:36: 3000000 INFO @ Mon, 29 Jun 2020 22:38:42: 9000000 INFO @ Mon, 29 Jun 2020 22:38:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:38:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:38:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:38:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:38:48: 10000000 INFO @ Mon, 29 Jun 2020 22:38:49: 5000000 INFO @ Mon, 29 Jun 2020 22:38:53: 1000000 INFO @ Mon, 29 Jun 2020 22:38:55: 11000000 INFO @ Mon, 29 Jun 2020 22:38:55: 6000000 INFO @ Mon, 29 Jun 2020 22:38:59: 2000000 INFO @ Mon, 29 Jun 2020 22:39:01: 12000000 INFO @ Mon, 29 Jun 2020 22:39:02: 7000000 INFO @ Mon, 29 Jun 2020 22:39:04: 3000000 INFO @ Mon, 29 Jun 2020 22:39:08: 8000000 INFO @ Mon, 29 Jun 2020 22:39:08: 13000000 INFO @ Mon, 29 Jun 2020 22:39:10: 4000000 INFO @ Mon, 29 Jun 2020 22:39:10: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:39:10: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:39:10: #1 total tags in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:39:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:39:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:39:10: #1 tags after filtering in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:39:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:39:10: #1 finished! INFO @ Mon, 29 Jun 2020 22:39:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:39:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:39:11: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:39:11: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:39:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:39:14: 9000000 INFO @ Mon, 29 Jun 2020 22:39:15: 5000000 INFO @ Mon, 29 Jun 2020 22:39:20: 6000000 INFO @ Mon, 29 Jun 2020 22:39:21: 10000000 INFO @ Mon, 29 Jun 2020 22:39:26: 7000000 INFO @ Mon, 29 Jun 2020 22:39:27: 11000000 INFO @ Mon, 29 Jun 2020 22:39:31: 8000000 INFO @ Mon, 29 Jun 2020 22:39:34: 12000000 INFO @ Mon, 29 Jun 2020 22:39:36: 9000000 INFO @ Mon, 29 Jun 2020 22:39:40: 13000000 INFO @ Mon, 29 Jun 2020 22:39:42: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:39:42: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:39:42: #1 total tags in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:39:42: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:39:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:39:42: 10000000 INFO @ Mon, 29 Jun 2020 22:39:42: #1 tags after filtering in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:39:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:39:42: #1 finished! INFO @ Mon, 29 Jun 2020 22:39:42: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:39:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:39:43: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:39:43: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:39:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:39:47: 11000000 INFO @ Mon, 29 Jun 2020 22:39:53: 12000000 INFO @ Mon, 29 Jun 2020 22:39:59: 13000000 INFO @ Mon, 29 Jun 2020 22:40:00: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:40:00: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:40:00: #1 total tags in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:40:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:40:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:40:00: #1 tags after filtering in treatment: 13216601 INFO @ Mon, 29 Jun 2020 22:40:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:40:00: #1 finished! INFO @ Mon, 29 Jun 2020 22:40:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:40:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:40:01: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:40:01: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:40:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2618502/SRX2618502.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。