Job ID = 1294352 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 36,056,600 reads read : 72,113,200 reads written : 72,113,200 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:31:35 36056600 reads; of these: 36056600 (100.00%) were paired; of these: 2374137 (6.58%) aligned concordantly 0 times 24863286 (68.96%) aligned concordantly exactly 1 time 8819177 (24.46%) aligned concordantly >1 times ---- 2374137 pairs aligned concordantly 0 times; of these: 554461 (23.35%) aligned discordantly 1 time ---- 1819676 pairs aligned 0 times concordantly or discordantly; of these: 3639352 mates make up the pairs; of these: 1719889 (47.26%) aligned 0 times 1004551 (27.60%) aligned exactly 1 time 914912 (25.14%) aligned >1 times 97.62% overall alignment rate Time searching: 01:31:35 Overall time: 01:31:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2327387 / 34102716 = 0.0682 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 09:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:11:23: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:11:23: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:11:23: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:11:23: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:11:23: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:11:23: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:11:32: 1000000 INFO @ Mon, 03 Jun 2019 09:11:32: 1000000 INFO @ Mon, 03 Jun 2019 09:11:32: 1000000 INFO @ Mon, 03 Jun 2019 09:11:40: 2000000 INFO @ Mon, 03 Jun 2019 09:11:41: 2000000 INFO @ Mon, 03 Jun 2019 09:11:41: 2000000 INFO @ Mon, 03 Jun 2019 09:11:49: 3000000 INFO @ Mon, 03 Jun 2019 09:11:50: 3000000 INFO @ Mon, 03 Jun 2019 09:11:50: 3000000 INFO @ Mon, 03 Jun 2019 09:11:57: 4000000 INFO @ Mon, 03 Jun 2019 09:11:58: 4000000 INFO @ Mon, 03 Jun 2019 09:11:59: 4000000 INFO @ Mon, 03 Jun 2019 09:12:06: 5000000 INFO @ Mon, 03 Jun 2019 09:12:06: 5000000 INFO @ Mon, 03 Jun 2019 09:12:09: 5000000 INFO @ Mon, 03 Jun 2019 09:12:15: 6000000 INFO @ Mon, 03 Jun 2019 09:12:15: 6000000 INFO @ Mon, 03 Jun 2019 09:12:18: 6000000 INFO @ Mon, 03 Jun 2019 09:12:24: 7000000 INFO @ Mon, 03 Jun 2019 09:12:24: 7000000 INFO @ Mon, 03 Jun 2019 09:12:27: 7000000 INFO @ Mon, 03 Jun 2019 09:12:32: 8000000 INFO @ Mon, 03 Jun 2019 09:12:33: 8000000 INFO @ Mon, 03 Jun 2019 09:12:36: 8000000 INFO @ Mon, 03 Jun 2019 09:12:41: 9000000 INFO @ Mon, 03 Jun 2019 09:12:41: 9000000 INFO @ Mon, 03 Jun 2019 09:12:45: 9000000 INFO @ Mon, 03 Jun 2019 09:12:50: 10000000 INFO @ Mon, 03 Jun 2019 09:12:50: 10000000 INFO @ Mon, 03 Jun 2019 09:12:54: 10000000 INFO @ Mon, 03 Jun 2019 09:12:58: 11000000 INFO @ Mon, 03 Jun 2019 09:12:59: 11000000 INFO @ Mon, 03 Jun 2019 09:13:03: 11000000 INFO @ Mon, 03 Jun 2019 09:13:07: 12000000 INFO @ Mon, 03 Jun 2019 09:13:08: 12000000 INFO @ Mon, 03 Jun 2019 09:13:12: 12000000 INFO @ Mon, 03 Jun 2019 09:13:15: 13000000 INFO @ Mon, 03 Jun 2019 09:13:17: 13000000 INFO @ Mon, 03 Jun 2019 09:13:21: 13000000 INFO @ Mon, 03 Jun 2019 09:13:23: 14000000 INFO @ Mon, 03 Jun 2019 09:13:26: 14000000 INFO @ Mon, 03 Jun 2019 09:13:30: 14000000 INFO @ Mon, 03 Jun 2019 09:13:31: 15000000 INFO @ Mon, 03 Jun 2019 09:13:34: 15000000 INFO @ Mon, 03 Jun 2019 09:13:39: 15000000 INFO @ Mon, 03 Jun 2019 09:13:40: 16000000 INFO @ Mon, 03 Jun 2019 09:13:42: 16000000 INFO @ Mon, 03 Jun 2019 09:13:47: 16000000 INFO @ Mon, 03 Jun 2019 09:13:48: 17000000 INFO @ Mon, 03 Jun 2019 09:13:51: 17000000 INFO @ Mon, 03 Jun 2019 09:13:55: 17000000 INFO @ Mon, 03 Jun 2019 09:13:56: 18000000 INFO @ Mon, 03 Jun 2019 09:14:00: 18000000 INFO @ Mon, 03 Jun 2019 09:14:04: 18000000 INFO @ Mon, 03 Jun 2019 09:14:05: 19000000 INFO @ Mon, 03 Jun 2019 09:14:09: 19000000 INFO @ Mon, 03 Jun 2019 09:14:12: 19000000 INFO @ Mon, 03 Jun 2019 09:14:14: 20000000 INFO @ Mon, 03 Jun 2019 09:14:17: 20000000 INFO @ Mon, 03 Jun 2019 09:14:20: 20000000 INFO @ Mon, 03 Jun 2019 09:14:22: 21000000 INFO @ Mon, 03 Jun 2019 09:14:26: 21000000 INFO @ Mon, 03 Jun 2019 09:14:29: 21000000 INFO @ Mon, 03 Jun 2019 09:14:30: 22000000 INFO @ Mon, 03 Jun 2019 09:14:35: 22000000 INFO @ Mon, 03 Jun 2019 09:14:37: 22000000 INFO @ Mon, 03 Jun 2019 09:14:39: 23000000 INFO @ Mon, 03 Jun 2019 09:14:43: 23000000 INFO @ Mon, 03 Jun 2019 09:14:46: 23000000 INFO @ Mon, 03 Jun 2019 09:14:47: 24000000 INFO @ Mon, 03 Jun 2019 09:14:51: 24000000 INFO @ Mon, 03 Jun 2019 09:14:54: 24000000 INFO @ Mon, 03 Jun 2019 09:14:56: 25000000 INFO @ Mon, 03 Jun 2019 09:15:01: 25000000 INFO @ Mon, 03 Jun 2019 09:15:02: 25000000 INFO @ Mon, 03 Jun 2019 09:15:04: 26000000 INFO @ Mon, 03 Jun 2019 09:15:09: 26000000 INFO @ Mon, 03 Jun 2019 09:15:10: 26000000 INFO @ Mon, 03 Jun 2019 09:15:13: 27000000 INFO @ Mon, 03 Jun 2019 09:15:18: 27000000 INFO @ Mon, 03 Jun 2019 09:15:18: 27000000 INFO @ Mon, 03 Jun 2019 09:15:21: 28000000 INFO @ Mon, 03 Jun 2019 09:15:26: 28000000 INFO @ Mon, 03 Jun 2019 09:15:26: 28000000 INFO @ Mon, 03 Jun 2019 09:15:30: 29000000 INFO @ Mon, 03 Jun 2019 09:15:35: 29000000 INFO @ Mon, 03 Jun 2019 09:15:35: 29000000 INFO @ Mon, 03 Jun 2019 09:15:38: 30000000 INFO @ Mon, 03 Jun 2019 09:15:43: 30000000 INFO @ Mon, 03 Jun 2019 09:15:43: 30000000 INFO @ Mon, 03 Jun 2019 09:15:47: 31000000 INFO @ Mon, 03 Jun 2019 09:15:52: 31000000 INFO @ Mon, 03 Jun 2019 09:15:52: 31000000 INFO @ Mon, 03 Jun 2019 09:15:56: 32000000 INFO @ Mon, 03 Jun 2019 09:16:01: 32000000 INFO @ Mon, 03 Jun 2019 09:16:02: 32000000 INFO @ Mon, 03 Jun 2019 09:16:05: 33000000 INFO @ Mon, 03 Jun 2019 09:16:10: 33000000 INFO @ Mon, 03 Jun 2019 09:16:13: 33000000 INFO @ Mon, 03 Jun 2019 09:16:14: 34000000 INFO @ Mon, 03 Jun 2019 09:16:19: 34000000 INFO @ Mon, 03 Jun 2019 09:16:23: 35000000 INFO @ Mon, 03 Jun 2019 09:16:23: 34000000 INFO @ Mon, 03 Jun 2019 09:16:29: 35000000 INFO @ Mon, 03 Jun 2019 09:16:32: 36000000 INFO @ Mon, 03 Jun 2019 09:16:33: 35000000 INFO @ Mon, 03 Jun 2019 09:16:37: 36000000 INFO @ Mon, 03 Jun 2019 09:16:41: 37000000 INFO @ Mon, 03 Jun 2019 09:16:44: 36000000 INFO @ Mon, 03 Jun 2019 09:16:46: 37000000 INFO @ Mon, 03 Jun 2019 09:16:50: 38000000 INFO @ Mon, 03 Jun 2019 09:16:53: 37000000 INFO @ Mon, 03 Jun 2019 09:16:55: 38000000 INFO @ Mon, 03 Jun 2019 09:16:59: 39000000 INFO @ Mon, 03 Jun 2019 09:17:03: 38000000 INFO @ Mon, 03 Jun 2019 09:17:03: 39000000 INFO @ Mon, 03 Jun 2019 09:17:08: 40000000 INFO @ Mon, 03 Jun 2019 09:17:12: 40000000 INFO @ Mon, 03 Jun 2019 09:17:13: 39000000 INFO @ Mon, 03 Jun 2019 09:17:17: 41000000 INFO @ Mon, 03 Jun 2019 09:17:20: 41000000 INFO @ Mon, 03 Jun 2019 09:17:23: 40000000 INFO @ Mon, 03 Jun 2019 09:17:26: 42000000 INFO @ Mon, 03 Jun 2019 09:17:29: 42000000 INFO @ Mon, 03 Jun 2019 09:17:32: 41000000 INFO @ Mon, 03 Jun 2019 09:17:35: 43000000 INFO @ Mon, 03 Jun 2019 09:17:37: 43000000 INFO @ Mon, 03 Jun 2019 09:17:41: 42000000 INFO @ Mon, 03 Jun 2019 09:17:44: 44000000 INFO @ Mon, 03 Jun 2019 09:17:46: 44000000 INFO @ Mon, 03 Jun 2019 09:17:50: 43000000 INFO @ Mon, 03 Jun 2019 09:17:53: 45000000 INFO @ Mon, 03 Jun 2019 09:17:55: 45000000 INFO @ Mon, 03 Jun 2019 09:17:59: 44000000 INFO @ Mon, 03 Jun 2019 09:18:02: 46000000 INFO @ Mon, 03 Jun 2019 09:18:03: 46000000 INFO @ Mon, 03 Jun 2019 09:18:08: 45000000 INFO @ Mon, 03 Jun 2019 09:18:11: 47000000 INFO @ Mon, 03 Jun 2019 09:18:12: 47000000 INFO @ Mon, 03 Jun 2019 09:18:17: 46000000 INFO @ Mon, 03 Jun 2019 09:18:19: 48000000 INFO @ Mon, 03 Jun 2019 09:18:21: 48000000 INFO @ Mon, 03 Jun 2019 09:18:27: 47000000 INFO @ Mon, 03 Jun 2019 09:18:28: 49000000 INFO @ Mon, 03 Jun 2019 09:18:31: 49000000 INFO @ Mon, 03 Jun 2019 09:18:36: 48000000 INFO @ Mon, 03 Jun 2019 09:18:38: 50000000 INFO @ Mon, 03 Jun 2019 09:18:41: 50000000 INFO @ Mon, 03 Jun 2019 09:18:44: 49000000 INFO @ Mon, 03 Jun 2019 09:18:47: 51000000 INFO @ Mon, 03 Jun 2019 09:18:50: 51000000 INFO @ Mon, 03 Jun 2019 09:18:53: 50000000 INFO @ Mon, 03 Jun 2019 09:18:56: 52000000 INFO @ Mon, 03 Jun 2019 09:18:59: 52000000 INFO @ Mon, 03 Jun 2019 09:19:02: 51000000 INFO @ Mon, 03 Jun 2019 09:19:06: 53000000 INFO @ Mon, 03 Jun 2019 09:19:09: 53000000 INFO @ Mon, 03 Jun 2019 09:19:10: 52000000 INFO @ Mon, 03 Jun 2019 09:19:15: 54000000 INFO @ Mon, 03 Jun 2019 09:19:18: 54000000 INFO @ Mon, 03 Jun 2019 09:19:19: 53000000 INFO @ Mon, 03 Jun 2019 09:19:24: 55000000 INFO @ Mon, 03 Jun 2019 09:19:26: 55000000 INFO @ Mon, 03 Jun 2019 09:19:28: 54000000 INFO @ Mon, 03 Jun 2019 09:19:32: 56000000 INFO @ Mon, 03 Jun 2019 09:19:35: 56000000 INFO @ Mon, 03 Jun 2019 09:19:37: 55000000 INFO @ Mon, 03 Jun 2019 09:19:41: 57000000 INFO @ Mon, 03 Jun 2019 09:19:44: 57000000 INFO @ Mon, 03 Jun 2019 09:19:45: 56000000 INFO @ Mon, 03 Jun 2019 09:19:50: 58000000 INFO @ Mon, 03 Jun 2019 09:19:53: 58000000 INFO @ Mon, 03 Jun 2019 09:19:54: 57000000 INFO @ Mon, 03 Jun 2019 09:20:00: 59000000 INFO @ Mon, 03 Jun 2019 09:20:03: 59000000 INFO @ Mon, 03 Jun 2019 09:20:03: 58000000 INFO @ Mon, 03 Jun 2019 09:20:09: 60000000 INFO @ Mon, 03 Jun 2019 09:20:11: 59000000 INFO @ Mon, 03 Jun 2019 09:20:12: 60000000 INFO @ Mon, 03 Jun 2019 09:20:17: 61000000 INFO @ Mon, 03 Jun 2019 09:20:20: 60000000 INFO @ Mon, 03 Jun 2019 09:20:20: 61000000 INFO @ Mon, 03 Jun 2019 09:20:26: 62000000 INFO @ Mon, 03 Jun 2019 09:20:28: 61000000 INFO @ Mon, 03 Jun 2019 09:20:29: 62000000 INFO @ Mon, 03 Jun 2019 09:20:35: 63000000 INFO @ Mon, 03 Jun 2019 09:20:38: 62000000 INFO @ Mon, 03 Jun 2019 09:20:38: 63000000 INFO @ Mon, 03 Jun 2019 09:20:44: 64000000 INFO @ Mon, 03 Jun 2019 09:20:46: 63000000 INFO @ Mon, 03 Jun 2019 09:20:48: 64000000 INFO @ Mon, 03 Jun 2019 09:20:54: 65000000 INFO @ Mon, 03 Jun 2019 09:20:55: 64000000 INFO @ Mon, 03 Jun 2019 09:20:57: 65000000 INFO @ Mon, 03 Jun 2019 09:21:01: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:21:01: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:21:01: #1 total tags in treatment: 31362559 INFO @ Mon, 03 Jun 2019 09:21:01: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:21:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:21:01: #1 tags after filtering in treatment: 28436938 INFO @ Mon, 03 Jun 2019 09:21:01: #1 Redundant rate of treatment: 0.09 INFO @ Mon, 03 Jun 2019 09:21:01: #1 finished! INFO @ Mon, 03 Jun 2019 09:21:01: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:21:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:21:03: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:21:03: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:21:03: #1 total tags in treatment: 31362559 INFO @ Mon, 03 Jun 2019 09:21:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:21:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:21:04: 65000000 INFO @ Mon, 03 Jun 2019 09:21:04: #2 number of paired peaks: 166 WARNING @ Mon, 03 Jun 2019 09:21:04: Fewer paired peaks (166) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 166 pairs to build model! INFO @ Mon, 03 Jun 2019 09:21:04: start model_add_line... INFO @ Mon, 03 Jun 2019 09:21:04: #1 tags after filtering in treatment: 28436938 INFO @ Mon, 03 Jun 2019 09:21:04: #1 Redundant rate of treatment: 0.09 INFO @ Mon, 03 Jun 2019 09:21:04: #1 finished! INFO @ Mon, 03 Jun 2019 09:21:04: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:21:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:21:04: start X-correlation... INFO @ Mon, 03 Jun 2019 09:21:04: end of X-cor INFO @ Mon, 03 Jun 2019 09:21:04: #2 finished! INFO @ Mon, 03 Jun 2019 09:21:04: #2 predicted fragment length is 162 bps INFO @ Mon, 03 Jun 2019 09:21:04: #2 alternative fragment length(s) may be 162,169 bps INFO @ Mon, 03 Jun 2019 09:21:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05_model.r INFO @ Mon, 03 Jun 2019 09:21:04: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:21:04: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:21:06: #2 number of paired peaks: 166 WARNING @ Mon, 03 Jun 2019 09:21:06: Fewer paired peaks (166) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 166 pairs to build model! INFO @ Mon, 03 Jun 2019 09:21:06: start model_add_line... INFO @ Mon, 03 Jun 2019 09:21:07: start X-correlation... INFO @ Mon, 03 Jun 2019 09:21:07: end of X-cor INFO @ Mon, 03 Jun 2019 09:21:07: #2 finished! INFO @ Mon, 03 Jun 2019 09:21:07: #2 predicted fragment length is 162 bps INFO @ Mon, 03 Jun 2019 09:21:07: #2 alternative fragment length(s) may be 162,169 bps INFO @ Mon, 03 Jun 2019 09:21:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20_model.r INFO @ Mon, 03 Jun 2019 09:21:07: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:21:07: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:21:10: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:21:10: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:21:10: #1 total tags in treatment: 31362559 INFO @ Mon, 03 Jun 2019 09:21:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:21:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:21:11: #1 tags after filtering in treatment: 28436938 INFO @ Mon, 03 Jun 2019 09:21:11: #1 Redundant rate of treatment: 0.09 INFO @ Mon, 03 Jun 2019 09:21:11: #1 finished! INFO @ Mon, 03 Jun 2019 09:21:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:21:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:21:13: #2 number of paired peaks: 166 WARNING @ Mon, 03 Jun 2019 09:21:13: Fewer paired peaks (166) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 166 pairs to build model! INFO @ Mon, 03 Jun 2019 09:21:13: start model_add_line... INFO @ Mon, 03 Jun 2019 09:21:14: start X-correlation... INFO @ Mon, 03 Jun 2019 09:21:14: end of X-cor INFO @ Mon, 03 Jun 2019 09:21:14: #2 finished! INFO @ Mon, 03 Jun 2019 09:21:14: #2 predicted fragment length is 162 bps INFO @ Mon, 03 Jun 2019 09:21:14: #2 alternative fragment length(s) may be 162,169 bps INFO @ Mon, 03 Jun 2019 09:21:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10_model.r INFO @ Mon, 03 Jun 2019 09:21:14: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:21:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:22:15: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:22:17: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:22:23: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:22:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05_peaks.xls INFO @ Mon, 03 Jun 2019 09:22:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:22:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.05_summits.bed INFO @ Mon, 03 Jun 2019 09:22:47: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2888 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 09:22:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20_peaks.xls INFO @ Mon, 03 Jun 2019 09:22:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:22:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.20_summits.bed INFO @ Mon, 03 Jun 2019 09:22:50: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (927 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 09:22:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10_peaks.xls INFO @ Mon, 03 Jun 2019 09:22:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:22:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612517/SRX2612517.10_summits.bed INFO @ Mon, 03 Jun 2019 09:22:57: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (1864 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。