Job ID = 12264834 SRX = SRX2612501 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 60742187 spots for SRR5312676/SRR5312676.sra Written 60742187 spots for SRR5312676/SRR5312676.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265943 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:05:36 60742187 reads; of these: 60742187 (100.00%) were paired; of these: 12294555 (20.24%) aligned concordantly 0 times 28772493 (47.37%) aligned concordantly exactly 1 time 19675139 (32.39%) aligned concordantly >1 times ---- 12294555 pairs aligned concordantly 0 times; of these: 4411507 (35.88%) aligned discordantly 1 time ---- 7883048 pairs aligned 0 times concordantly or discordantly; of these: 15766096 mates make up the pairs; of these: 9707773 (61.57%) aligned 0 times 1895347 (12.02%) aligned exactly 1 time 4162976 (26.40%) aligned >1 times 92.01% overall alignment rate Time searching: 02:05:37 Overall time: 02:05:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 23808894 / 52296851 = 0.4553 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:03:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:03:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:03:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:03:36: 1000000 INFO @ Sat, 03 Apr 2021 09:03:42: 2000000 INFO @ Sat, 03 Apr 2021 09:03:49: 3000000 INFO @ Sat, 03 Apr 2021 09:03:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:04:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:04:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:04:01: 5000000 INFO @ Sat, 03 Apr 2021 09:04:07: 1000000 INFO @ Sat, 03 Apr 2021 09:04:08: 6000000 INFO @ Sat, 03 Apr 2021 09:04:14: 7000000 INFO @ Sat, 03 Apr 2021 09:04:15: 2000000 INFO @ Sat, 03 Apr 2021 09:04:20: 8000000 INFO @ Sat, 03 Apr 2021 09:04:22: 3000000 INFO @ Sat, 03 Apr 2021 09:04:26: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:04:29: 4000000 INFO @ Sat, 03 Apr 2021 09:04:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:04:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:04:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:04:32: 10000000 INFO @ Sat, 03 Apr 2021 09:04:36: 5000000 INFO @ Sat, 03 Apr 2021 09:04:37: 1000000 INFO @ Sat, 03 Apr 2021 09:04:38: 11000000 INFO @ Sat, 03 Apr 2021 09:04:44: 6000000 INFO @ Sat, 03 Apr 2021 09:04:45: 2000000 INFO @ Sat, 03 Apr 2021 09:04:45: 12000000 INFO @ Sat, 03 Apr 2021 09:04:51: 7000000 INFO @ Sat, 03 Apr 2021 09:04:51: 13000000 INFO @ Sat, 03 Apr 2021 09:04:52: 3000000 INFO @ Sat, 03 Apr 2021 09:04:57: 14000000 INFO @ Sat, 03 Apr 2021 09:04:58: 8000000 INFO @ Sat, 03 Apr 2021 09:04:59: 4000000 INFO @ Sat, 03 Apr 2021 09:05:03: 15000000 INFO @ Sat, 03 Apr 2021 09:05:05: 9000000 INFO @ Sat, 03 Apr 2021 09:05:06: 5000000 INFO @ Sat, 03 Apr 2021 09:05:09: 16000000 INFO @ Sat, 03 Apr 2021 09:05:12: 10000000 INFO @ Sat, 03 Apr 2021 09:05:13: 6000000 INFO @ Sat, 03 Apr 2021 09:05:15: 17000000 INFO @ Sat, 03 Apr 2021 09:05:18: 11000000 INFO @ Sat, 03 Apr 2021 09:05:20: 7000000 INFO @ Sat, 03 Apr 2021 09:05:22: 18000000 INFO @ Sat, 03 Apr 2021 09:05:25: 12000000 INFO @ Sat, 03 Apr 2021 09:05:27: 8000000 INFO @ Sat, 03 Apr 2021 09:05:28: 19000000 INFO @ Sat, 03 Apr 2021 09:05:32: 13000000 INFO @ Sat, 03 Apr 2021 09:05:34: 20000000 INFO @ Sat, 03 Apr 2021 09:05:34: 9000000 INFO @ Sat, 03 Apr 2021 09:05:39: 14000000 INFO @ Sat, 03 Apr 2021 09:05:40: 21000000 INFO @ Sat, 03 Apr 2021 09:05:41: 10000000 INFO @ Sat, 03 Apr 2021 09:05:46: 15000000 INFO @ Sat, 03 Apr 2021 09:05:46: 22000000 INFO @ Sat, 03 Apr 2021 09:05:47: 11000000 INFO @ Sat, 03 Apr 2021 09:05:52: 23000000 INFO @ Sat, 03 Apr 2021 09:05:53: 16000000 INFO @ Sat, 03 Apr 2021 09:05:54: 12000000 INFO @ Sat, 03 Apr 2021 09:05:58: 24000000 INFO @ Sat, 03 Apr 2021 09:06:00: 17000000 INFO @ Sat, 03 Apr 2021 09:06:01: 13000000 INFO @ Sat, 03 Apr 2021 09:06:04: 25000000 INFO @ Sat, 03 Apr 2021 09:06:06: 18000000 INFO @ Sat, 03 Apr 2021 09:06:08: 14000000 INFO @ Sat, 03 Apr 2021 09:06:11: 26000000 INFO @ Sat, 03 Apr 2021 09:06:13: 19000000 INFO @ Sat, 03 Apr 2021 09:06:14: 15000000 INFO @ Sat, 03 Apr 2021 09:06:17: 27000000 INFO @ Sat, 03 Apr 2021 09:06:20: 20000000 INFO @ Sat, 03 Apr 2021 09:06:21: 16000000 INFO @ Sat, 03 Apr 2021 09:06:23: 28000000 INFO @ Sat, 03 Apr 2021 09:06:27: 21000000 INFO @ Sat, 03 Apr 2021 09:06:28: 17000000 INFO @ Sat, 03 Apr 2021 09:06:29: 29000000 INFO @ Sat, 03 Apr 2021 09:06:34: 22000000 INFO @ Sat, 03 Apr 2021 09:06:35: 18000000 INFO @ Sat, 03 Apr 2021 09:06:35: 30000000 INFO @ Sat, 03 Apr 2021 09:06:40: 23000000 INFO @ Sat, 03 Apr 2021 09:06:41: 31000000 INFO @ Sat, 03 Apr 2021 09:06:41: 19000000 INFO @ Sat, 03 Apr 2021 09:06:47: 24000000 INFO @ Sat, 03 Apr 2021 09:06:47: 32000000 INFO @ Sat, 03 Apr 2021 09:06:48: 20000000 INFO @ Sat, 03 Apr 2021 09:06:53: 33000000 INFO @ Sat, 03 Apr 2021 09:06:54: 25000000 INFO @ Sat, 03 Apr 2021 09:06:55: 21000000 INFO @ Sat, 03 Apr 2021 09:07:00: 34000000 INFO @ Sat, 03 Apr 2021 09:07:01: 26000000 INFO @ Sat, 03 Apr 2021 09:07:01: 22000000 INFO @ Sat, 03 Apr 2021 09:07:06: 35000000 INFO @ Sat, 03 Apr 2021 09:07:08: 27000000 INFO @ Sat, 03 Apr 2021 09:07:08: 23000000 INFO @ Sat, 03 Apr 2021 09:07:12: 36000000 INFO @ Sat, 03 Apr 2021 09:07:14: 28000000 INFO @ Sat, 03 Apr 2021 09:07:15: 24000000 INFO @ Sat, 03 Apr 2021 09:07:18: 37000000 INFO @ Sat, 03 Apr 2021 09:07:21: 29000000 INFO @ Sat, 03 Apr 2021 09:07:21: 25000000 INFO @ Sat, 03 Apr 2021 09:07:25: 38000000 INFO @ Sat, 03 Apr 2021 09:07:28: 30000000 INFO @ Sat, 03 Apr 2021 09:07:28: 26000000 INFO @ Sat, 03 Apr 2021 09:07:31: 39000000 INFO @ Sat, 03 Apr 2021 09:07:35: 27000000 INFO @ Sat, 03 Apr 2021 09:07:35: 31000000 INFO @ Sat, 03 Apr 2021 09:07:37: 40000000 INFO @ Sat, 03 Apr 2021 09:07:42: 28000000 INFO @ Sat, 03 Apr 2021 09:07:42: 32000000 INFO @ Sat, 03 Apr 2021 09:07:43: 41000000 INFO @ Sat, 03 Apr 2021 09:07:48: 29000000 INFO @ Sat, 03 Apr 2021 09:07:49: 33000000 INFO @ Sat, 03 Apr 2021 09:07:50: 42000000 INFO @ Sat, 03 Apr 2021 09:07:55: 30000000 INFO @ Sat, 03 Apr 2021 09:07:55: 34000000 INFO @ Sat, 03 Apr 2021 09:07:56: 43000000 INFO @ Sat, 03 Apr 2021 09:08:02: 31000000 INFO @ Sat, 03 Apr 2021 09:08:02: 44000000 INFO @ Sat, 03 Apr 2021 09:08:03: 35000000 INFO @ Sat, 03 Apr 2021 09:08:08: 45000000 INFO @ Sat, 03 Apr 2021 09:08:08: 32000000 INFO @ Sat, 03 Apr 2021 09:08:10: 36000000 INFO @ Sat, 03 Apr 2021 09:08:13: 46000000 INFO @ Sat, 03 Apr 2021 09:08:15: 33000000 INFO @ Sat, 03 Apr 2021 09:08:17: 37000000 INFO @ Sat, 03 Apr 2021 09:08:20: 47000000 INFO @ Sat, 03 Apr 2021 09:08:22: 34000000 INFO @ Sat, 03 Apr 2021 09:08:24: 38000000 INFO @ Sat, 03 Apr 2021 09:08:26: 48000000 INFO @ Sat, 03 Apr 2021 09:08:29: 35000000 INFO @ Sat, 03 Apr 2021 09:08:31: 39000000 INFO @ Sat, 03 Apr 2021 09:08:32: 49000000 INFO @ Sat, 03 Apr 2021 09:08:36: 36000000 INFO @ Sat, 03 Apr 2021 09:08:38: 50000000 INFO @ Sat, 03 Apr 2021 09:08:38: 40000000 INFO @ Sat, 03 Apr 2021 09:08:42: 37000000 INFO @ Sat, 03 Apr 2021 09:08:44: 51000000 INFO @ Sat, 03 Apr 2021 09:08:45: 41000000 INFO @ Sat, 03 Apr 2021 09:08:49: 38000000 INFO @ Sat, 03 Apr 2021 09:08:50: 52000000 INFO @ Sat, 03 Apr 2021 09:08:52: 42000000 INFO @ Sat, 03 Apr 2021 09:08:56: 39000000 INFO @ Sat, 03 Apr 2021 09:08:56: 53000000 INFO @ Sat, 03 Apr 2021 09:08:59: 43000000 INFO @ Sat, 03 Apr 2021 09:09:03: 54000000 INFO @ Sat, 03 Apr 2021 09:09:03: 40000000 INFO @ Sat, 03 Apr 2021 09:09:06: 44000000 INFO @ Sat, 03 Apr 2021 09:09:09: 55000000 INFO @ Sat, 03 Apr 2021 09:09:10: 41000000 INFO @ Sat, 03 Apr 2021 09:09:12: 45000000 INFO @ Sat, 03 Apr 2021 09:09:15: 56000000 INFO @ Sat, 03 Apr 2021 09:09:17: 42000000 INFO @ Sat, 03 Apr 2021 09:09:19: 46000000 INFO @ Sat, 03 Apr 2021 09:09:21: 57000000 INFO @ Sat, 03 Apr 2021 09:09:23: 43000000 INFO @ Sat, 03 Apr 2021 09:09:26: 47000000 INFO @ Sat, 03 Apr 2021 09:09:28: 58000000 INFO @ Sat, 03 Apr 2021 09:09:30: 44000000 INFO @ Sat, 03 Apr 2021 09:09:32: 48000000 INFO @ Sat, 03 Apr 2021 09:09:34: 59000000 INFO @ Sat, 03 Apr 2021 09:09:36: 45000000 INFO @ Sat, 03 Apr 2021 09:09:39: 49000000 INFO @ Sat, 03 Apr 2021 09:09:40: 60000000 INFO @ Sat, 03 Apr 2021 09:09:43: 46000000 INFO @ Sat, 03 Apr 2021 09:09:46: 50000000 INFO @ Sat, 03 Apr 2021 09:09:46: 61000000 INFO @ Sat, 03 Apr 2021 09:09:49: 47000000 INFO @ Sat, 03 Apr 2021 09:09:53: 51000000 INFO @ Sat, 03 Apr 2021 09:09:53: 62000000 INFO @ Sat, 03 Apr 2021 09:09:56: 48000000 INFO @ Sat, 03 Apr 2021 09:09:59: 63000000 INFO @ Sat, 03 Apr 2021 09:09:59: 52000000 INFO @ Sat, 03 Apr 2021 09:10:02: 49000000 INFO @ Sat, 03 Apr 2021 09:10:06: 64000000 INFO @ Sat, 03 Apr 2021 09:10:06: 53000000 INFO @ Sat, 03 Apr 2021 09:10:07: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 09:10:07: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 09:10:07: #1 total tags in treatment: 25856152 INFO @ Sat, 03 Apr 2021 09:10:07: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:10:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:10:08: #1 tags after filtering in treatment: 18853981 INFO @ Sat, 03 Apr 2021 09:10:08: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 03 Apr 2021 09:10:08: #1 finished! INFO @ Sat, 03 Apr 2021 09:10:08: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:10:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:10:09: 50000000 INFO @ Sat, 03 Apr 2021 09:10:09: #2 number of paired peaks: 215 WARNING @ Sat, 03 Apr 2021 09:10:09: Fewer paired peaks (215) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 215 pairs to build model! INFO @ Sat, 03 Apr 2021 09:10:09: start model_add_line... INFO @ Sat, 03 Apr 2021 09:10:09: start X-correlation... INFO @ Sat, 03 Apr 2021 09:10:09: end of X-cor INFO @ Sat, 03 Apr 2021 09:10:09: #2 finished! INFO @ Sat, 03 Apr 2021 09:10:09: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 09:10:09: #2 alternative fragment length(s) may be 3,56,84 bps INFO @ Sat, 03 Apr 2021 09:10:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05_model.r WARNING @ Sat, 03 Apr 2021 09:10:09: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:10:09: #2 You may need to consider one of the other alternative d(s): 3,56,84 WARNING @ Sat, 03 Apr 2021 09:10:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:10:09: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:10:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:10:13: 54000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:10:16: 51000000 INFO @ Sat, 03 Apr 2021 09:10:20: 55000000 INFO @ Sat, 03 Apr 2021 09:10:22: 52000000 INFO @ Sat, 03 Apr 2021 09:10:27: 56000000 INFO @ Sat, 03 Apr 2021 09:10:29: 53000000 INFO @ Sat, 03 Apr 2021 09:10:34: 57000000 INFO @ Sat, 03 Apr 2021 09:10:36: 54000000 INFO @ Sat, 03 Apr 2021 09:10:41: 58000000 INFO @ Sat, 03 Apr 2021 09:10:43: 55000000 INFO @ Sat, 03 Apr 2021 09:10:49: 59000000 INFO @ Sat, 03 Apr 2021 09:10:50: 56000000 INFO @ Sat, 03 Apr 2021 09:10:56: 60000000 INFO @ Sat, 03 Apr 2021 09:10:57: 57000000 INFO @ Sat, 03 Apr 2021 09:10:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:11:03: 61000000 INFO @ Sat, 03 Apr 2021 09:11:05: 58000000 INFO @ Sat, 03 Apr 2021 09:11:11: 62000000 INFO @ Sat, 03 Apr 2021 09:11:12: 59000000 INFO @ Sat, 03 Apr 2021 09:11:18: 63000000 INFO @ Sat, 03 Apr 2021 09:11:19: 60000000 INFO @ Sat, 03 Apr 2021 09:11:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:11:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:11:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.05_summits.bed INFO @ Sat, 03 Apr 2021 09:11:25: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (6555 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:11:26: 64000000 INFO @ Sat, 03 Apr 2021 09:11:26: 61000000 INFO @ Sat, 03 Apr 2021 09:11:28: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 09:11:28: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 09:11:28: #1 total tags in treatment: 25856152 INFO @ Sat, 03 Apr 2021 09:11:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:11:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:11:28: #1 tags after filtering in treatment: 18853981 INFO @ Sat, 03 Apr 2021 09:11:28: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 03 Apr 2021 09:11:28: #1 finished! INFO @ Sat, 03 Apr 2021 09:11:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:11:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:11:30: #2 number of paired peaks: 215 WARNING @ Sat, 03 Apr 2021 09:11:30: Fewer paired peaks (215) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 215 pairs to build model! INFO @ Sat, 03 Apr 2021 09:11:30: start model_add_line... INFO @ Sat, 03 Apr 2021 09:11:30: start X-correlation... INFO @ Sat, 03 Apr 2021 09:11:30: end of X-cor INFO @ Sat, 03 Apr 2021 09:11:30: #2 finished! INFO @ Sat, 03 Apr 2021 09:11:30: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 09:11:30: #2 alternative fragment length(s) may be 3,56,84 bps INFO @ Sat, 03 Apr 2021 09:11:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10_model.r WARNING @ Sat, 03 Apr 2021 09:11:30: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:11:30: #2 You may need to consider one of the other alternative d(s): 3,56,84 WARNING @ Sat, 03 Apr 2021 09:11:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:11:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:11:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:11:33: 62000000 INFO @ Sat, 03 Apr 2021 09:11:40: 63000000 INFO @ Sat, 03 Apr 2021 09:11:47: 64000000 INFO @ Sat, 03 Apr 2021 09:11:49: #1 tag size is determined as 51 bps INFO @ Sat, 03 Apr 2021 09:11:49: #1 tag size = 51 INFO @ Sat, 03 Apr 2021 09:11:49: #1 total tags in treatment: 25856152 INFO @ Sat, 03 Apr 2021 09:11:49: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:11:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:11:49: #1 tags after filtering in treatment: 18853981 INFO @ Sat, 03 Apr 2021 09:11:49: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 03 Apr 2021 09:11:49: #1 finished! INFO @ Sat, 03 Apr 2021 09:11:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:11:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:11:51: #2 number of paired peaks: 215 WARNING @ Sat, 03 Apr 2021 09:11:51: Fewer paired peaks (215) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 215 pairs to build model! INFO @ Sat, 03 Apr 2021 09:11:51: start model_add_line... INFO @ Sat, 03 Apr 2021 09:11:51: start X-correlation... INFO @ Sat, 03 Apr 2021 09:11:51: end of X-cor INFO @ Sat, 03 Apr 2021 09:11:51: #2 finished! INFO @ Sat, 03 Apr 2021 09:11:51: #2 predicted fragment length is 84 bps INFO @ Sat, 03 Apr 2021 09:11:51: #2 alternative fragment length(s) may be 3,56,84 bps INFO @ Sat, 03 Apr 2021 09:11:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20_model.r WARNING @ Sat, 03 Apr 2021 09:11:51: #2 Since the d (84) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:11:51: #2 You may need to consider one of the other alternative d(s): 3,56,84 WARNING @ Sat, 03 Apr 2021 09:11:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:11:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:11:51: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:12:20: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:12:42: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:12:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:12:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:12:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.10_summits.bed INFO @ Sat, 03 Apr 2021 09:12:46: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2810 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:13:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:13:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:13:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612501/SRX2612501.20_summits.bed INFO @ Sat, 03 Apr 2021 09:13:09: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (912 records, 4 fields): 5 millis CompletedMACS2peakCalling