Job ID = 11171173 sra ファイルのダウンロード中... Completed: 1364580K bytes transferred in 66 seconds (168675K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 47692266 spots for /home/okishinya/chipatlas/results/dm3/SRX2564146/SRR5259265.sra Written 47692266 spots for /home/okishinya/chipatlas/results/dm3/SRX2564146/SRR5259265.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:48 47692266 reads; of these: 47692266 (100.00%) were unpaired; of these: 3549052 (7.44%) aligned 0 times 32494989 (68.13%) aligned exactly 1 time 11648225 (24.42%) aligned >1 times 92.56% overall alignment rate Time searching: 00:23:48 Overall time: 00:23:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11468646 / 44143214 = 0.2598 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:07:10: # Command line: callpeak -t SRX2564146.bam -f BAM -g dm -n SRX2564146.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2564146.20 # format = BAM # ChIP-seq file = ['SRX2564146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:07:10: # Command line: callpeak -t SRX2564146.bam -f BAM -g dm -n SRX2564146.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2564146.05 # format = BAM # ChIP-seq file = ['SRX2564146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:07:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:07:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:07:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:07:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:07:10: # Command line: callpeak -t SRX2564146.bam -f BAM -g dm -n SRX2564146.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2564146.10 # format = BAM # ChIP-seq file = ['SRX2564146.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:07:10: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:07:10: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:07:17: 1000000 INFO @ Sat, 08 Sep 2018 13:07:17: 1000000 INFO @ Sat, 08 Sep 2018 13:07:17: 1000000 INFO @ Sat, 08 Sep 2018 13:07:25: 2000000 INFO @ Sat, 08 Sep 2018 13:07:25: 2000000 INFO @ Sat, 08 Sep 2018 13:07:25: 2000000 INFO @ Sat, 08 Sep 2018 13:07:32: 3000000 INFO @ Sat, 08 Sep 2018 13:07:33: 3000000 INFO @ Sat, 08 Sep 2018 13:07:33: 3000000 INFO @ Sat, 08 Sep 2018 13:07:40: 4000000 INFO @ Sat, 08 Sep 2018 13:07:40: 4000000 INFO @ Sat, 08 Sep 2018 13:07:41: 4000000 INFO @ Sat, 08 Sep 2018 13:07:48: 5000000 INFO @ Sat, 08 Sep 2018 13:07:48: 5000000 INFO @ Sat, 08 Sep 2018 13:07:48: 5000000 INFO @ Sat, 08 Sep 2018 13:07:55: 6000000 INFO @ Sat, 08 Sep 2018 13:07:56: 6000000 INFO @ Sat, 08 Sep 2018 13:07:56: 6000000 INFO @ Sat, 08 Sep 2018 13:08:03: 7000000 INFO @ Sat, 08 Sep 2018 13:08:03: 7000000 INFO @ Sat, 08 Sep 2018 13:08:04: 7000000 INFO @ Sat, 08 Sep 2018 13:08:10: 8000000 INFO @ Sat, 08 Sep 2018 13:08:11: 8000000 INFO @ Sat, 08 Sep 2018 13:08:11: 8000000 INFO @ Sat, 08 Sep 2018 13:08:18: 9000000 INFO @ Sat, 08 Sep 2018 13:08:19: 9000000 INFO @ Sat, 08 Sep 2018 13:08:19: 9000000 INFO @ Sat, 08 Sep 2018 13:08:25: 10000000 INFO @ Sat, 08 Sep 2018 13:08:26: 10000000 INFO @ Sat, 08 Sep 2018 13:08:27: 10000000 INFO @ Sat, 08 Sep 2018 13:08:33: 11000000 INFO @ Sat, 08 Sep 2018 13:08:34: 11000000 INFO @ Sat, 08 Sep 2018 13:08:35: 11000000 INFO @ Sat, 08 Sep 2018 13:08:40: 12000000 INFO @ Sat, 08 Sep 2018 13:08:42: 12000000 INFO @ Sat, 08 Sep 2018 13:08:42: 12000000 INFO @ Sat, 08 Sep 2018 13:08:48: 13000000 INFO @ Sat, 08 Sep 2018 13:08:50: 13000000 INFO @ Sat, 08 Sep 2018 13:08:50: 13000000 INFO @ Sat, 08 Sep 2018 13:08:55: 14000000 INFO @ Sat, 08 Sep 2018 13:08:57: 14000000 INFO @ Sat, 08 Sep 2018 13:08:58: 14000000 INFO @ Sat, 08 Sep 2018 13:09:03: 15000000 INFO @ Sat, 08 Sep 2018 13:09:05: 15000000 INFO @ Sat, 08 Sep 2018 13:09:05: 15000000 INFO @ Sat, 08 Sep 2018 13:09:10: 16000000 INFO @ Sat, 08 Sep 2018 13:09:13: 16000000 INFO @ Sat, 08 Sep 2018 13:09:13: 16000000 INFO @ Sat, 08 Sep 2018 13:09:17: 17000000 INFO @ Sat, 08 Sep 2018 13:09:20: 17000000 INFO @ Sat, 08 Sep 2018 13:09:21: 17000000 INFO @ Sat, 08 Sep 2018 13:09:25: 18000000 INFO @ Sat, 08 Sep 2018 13:09:28: 18000000 INFO @ Sat, 08 Sep 2018 13:09:29: 18000000 INFO @ Sat, 08 Sep 2018 13:09:32: 19000000 INFO @ Sat, 08 Sep 2018 13:09:36: 19000000 INFO @ Sat, 08 Sep 2018 13:09:36: 19000000 INFO @ Sat, 08 Sep 2018 13:09:40: 20000000 INFO @ Sat, 08 Sep 2018 13:09:43: 20000000 INFO @ Sat, 08 Sep 2018 13:09:44: 20000000 INFO @ Sat, 08 Sep 2018 13:09:47: 21000000 INFO @ Sat, 08 Sep 2018 13:09:51: 21000000 INFO @ Sat, 08 Sep 2018 13:09:52: 21000000 INFO @ Sat, 08 Sep 2018 13:09:55: 22000000 INFO @ Sat, 08 Sep 2018 13:09:59: 22000000 INFO @ Sat, 08 Sep 2018 13:10:00: 22000000 INFO @ Sat, 08 Sep 2018 13:10:02: 23000000 INFO @ Sat, 08 Sep 2018 13:10:07: 23000000 INFO @ Sat, 08 Sep 2018 13:10:08: 23000000 INFO @ Sat, 08 Sep 2018 13:10:10: 24000000 INFO @ Sat, 08 Sep 2018 13:10:15: 24000000 INFO @ Sat, 08 Sep 2018 13:10:16: 24000000 INFO @ Sat, 08 Sep 2018 13:10:17: 25000000 INFO @ Sat, 08 Sep 2018 13:10:23: 25000000 INFO @ Sat, 08 Sep 2018 13:10:24: 25000000 INFO @ Sat, 08 Sep 2018 13:10:25: 26000000 INFO @ Sat, 08 Sep 2018 13:10:31: 26000000 INFO @ Sat, 08 Sep 2018 13:10:32: 26000000 INFO @ Sat, 08 Sep 2018 13:10:33: 27000000 INFO @ Sat, 08 Sep 2018 13:10:39: 27000000 INFO @ Sat, 08 Sep 2018 13:10:40: 28000000 INFO @ Sat, 08 Sep 2018 13:10:40: 27000000 INFO @ Sat, 08 Sep 2018 13:10:47: 28000000 INFO @ Sat, 08 Sep 2018 13:10:48: 29000000 INFO @ Sat, 08 Sep 2018 13:10:49: 28000000 INFO @ Sat, 08 Sep 2018 13:10:55: 30000000 INFO @ Sat, 08 Sep 2018 13:10:55: 29000000 INFO @ Sat, 08 Sep 2018 13:10:57: 29000000 INFO @ Sat, 08 Sep 2018 13:11:03: 31000000 INFO @ Sat, 08 Sep 2018 13:11:03: 30000000 INFO @ Sat, 08 Sep 2018 13:11:05: 30000000 INFO @ Sat, 08 Sep 2018 13:11:10: 32000000 INFO @ Sat, 08 Sep 2018 13:11:12: 31000000 INFO @ Sat, 08 Sep 2018 13:11:13: 31000000 INFO @ Sat, 08 Sep 2018 13:11:16: #1 tag size is determined as 74 bps INFO @ Sat, 08 Sep 2018 13:11:16: #1 tag size = 74 INFO @ Sat, 08 Sep 2018 13:11:16: #1 total tags in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:16: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:11:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:11:16: #1 tags after filtering in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:11:16: #1 finished! INFO @ Sat, 08 Sep 2018 13:11:16: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:11:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:11:18: #2 number of paired peaks: 41 WARNING @ Sat, 08 Sep 2018 13:11:18: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:11:18: Process for pairing-model is terminated! cat: SRX2564146.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564146.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:11:20: 32000000 INFO @ Sat, 08 Sep 2018 13:11:21: 32000000 INFO @ Sat, 08 Sep 2018 13:11:25: #1 tag size is determined as 74 bps INFO @ Sat, 08 Sep 2018 13:11:25: #1 tag size = 74 INFO @ Sat, 08 Sep 2018 13:11:25: #1 total tags in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:25: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:11:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:11:26: #1 tags after filtering in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:11:26: #1 finished! INFO @ Sat, 08 Sep 2018 13:11:26: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:11:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:11:27: #1 tag size is determined as 74 bps INFO @ Sat, 08 Sep 2018 13:11:27: #1 tag size = 74 INFO @ Sat, 08 Sep 2018 13:11:27: #1 total tags in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:27: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:11:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:11:27: #1 tags after filtering in treatment: 32674568 INFO @ Sat, 08 Sep 2018 13:11:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:11:27: #1 finished! INFO @ Sat, 08 Sep 2018 13:11:27: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:11:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:11:28: #2 number of paired peaks: 41 WARNING @ Sat, 08 Sep 2018 13:11:28: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:11:28: Process for pairing-model is terminated! cat: SRX2564146.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564146.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:11:29: #2 number of paired peaks: 41 WARNING @ Sat, 08 Sep 2018 13:11:29: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:11:29: Process for pairing-model is terminated! cat: SRX2564146.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564146.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564146.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。