Job ID = 6527717 SRX = SRX245838 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:16:53 prefetch.2.10.7: 1) Downloading 'SRR765858'... 2020-06-29T13:16:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:20:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:20:10 prefetch.2.10.7: 1) 'SRR765858' was downloaded successfully Read 26465335 spots for SRR765858/SRR765858.sra Written 26465335 spots for SRR765858/SRR765858.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 26465335 reads; of these: 26465335 (100.00%) were unpaired; of these: 4578176 (17.30%) aligned 0 times 17199247 (64.99%) aligned exactly 1 time 4687912 (17.71%) aligned >1 times 82.70% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2059063 / 21887159 = 0.0941 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:36:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:36:11: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:36:11: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:36:16: 1000000 INFO @ Mon, 29 Jun 2020 22:36:22: 2000000 INFO @ Mon, 29 Jun 2020 22:36:27: 3000000 INFO @ Mon, 29 Jun 2020 22:36:32: 4000000 INFO @ Mon, 29 Jun 2020 22:36:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:36:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:36:40: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:36:40: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:36:43: 6000000 INFO @ Mon, 29 Jun 2020 22:36:45: 1000000 INFO @ Mon, 29 Jun 2020 22:36:49: 7000000 INFO @ Mon, 29 Jun 2020 22:36:50: 2000000 INFO @ Mon, 29 Jun 2020 22:36:54: 8000000 INFO @ Mon, 29 Jun 2020 22:36:55: 3000000 INFO @ Mon, 29 Jun 2020 22:36:59: 9000000 INFO @ Mon, 29 Jun 2020 22:37:00: 4000000 INFO @ Mon, 29 Jun 2020 22:37:05: 10000000 INFO @ Mon, 29 Jun 2020 22:37:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:37:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:37:10: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:37:10: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:37:10: 11000000 INFO @ Mon, 29 Jun 2020 22:37:10: 6000000 INFO @ Mon, 29 Jun 2020 22:37:15: 7000000 INFO @ Mon, 29 Jun 2020 22:37:16: 1000000 INFO @ Mon, 29 Jun 2020 22:37:16: 12000000 INFO @ Mon, 29 Jun 2020 22:37:20: 8000000 INFO @ Mon, 29 Jun 2020 22:37:21: 2000000 INFO @ Mon, 29 Jun 2020 22:37:21: 13000000 INFO @ Mon, 29 Jun 2020 22:37:25: 9000000 INFO @ Mon, 29 Jun 2020 22:37:26: 3000000 INFO @ Mon, 29 Jun 2020 22:37:27: 14000000 INFO @ Mon, 29 Jun 2020 22:37:31: 10000000 INFO @ Mon, 29 Jun 2020 22:37:32: 4000000 INFO @ Mon, 29 Jun 2020 22:37:32: 15000000 INFO @ Mon, 29 Jun 2020 22:37:35: 11000000 INFO @ Mon, 29 Jun 2020 22:37:37: 5000000 INFO @ Mon, 29 Jun 2020 22:37:38: 16000000 INFO @ Mon, 29 Jun 2020 22:37:40: 12000000 INFO @ Mon, 29 Jun 2020 22:37:43: 6000000 INFO @ Mon, 29 Jun 2020 22:37:43: 17000000 INFO @ Mon, 29 Jun 2020 22:37:45: 13000000 INFO @ Mon, 29 Jun 2020 22:37:48: 7000000 INFO @ Mon, 29 Jun 2020 22:37:49: 18000000 INFO @ Mon, 29 Jun 2020 22:37:50: 14000000 INFO @ Mon, 29 Jun 2020 22:37:53: 8000000 INFO @ Mon, 29 Jun 2020 22:37:54: 19000000 INFO @ Mon, 29 Jun 2020 22:37:56: 15000000 INFO @ Mon, 29 Jun 2020 22:37:59: 9000000 INFO @ Mon, 29 Jun 2020 22:37:59: #1 tag size is determined as 42 bps INFO @ Mon, 29 Jun 2020 22:37:59: #1 tag size = 42 INFO @ Mon, 29 Jun 2020 22:37:59: #1 total tags in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:37:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:37:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:37:59: #1 tags after filtering in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:37:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:37:59: #1 finished! INFO @ Mon, 29 Jun 2020 22:37:59: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:37:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:38:01: 16000000 INFO @ Mon, 29 Jun 2020 22:38:01: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 22:38:01: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:38:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:38:04: 10000000 INFO @ Mon, 29 Jun 2020 22:38:06: 17000000 INFO @ Mon, 29 Jun 2020 22:38:09: 11000000 INFO @ Mon, 29 Jun 2020 22:38:11: 18000000 INFO @ Mon, 29 Jun 2020 22:38:15: 12000000 INFO @ Mon, 29 Jun 2020 22:38:16: 19000000 INFO @ Mon, 29 Jun 2020 22:38:20: #1 tag size is determined as 42 bps INFO @ Mon, 29 Jun 2020 22:38:20: #1 tag size = 42 INFO @ Mon, 29 Jun 2020 22:38:20: #1 total tags in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:38:20: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:38:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:38:20: #1 tags after filtering in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:38:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:38:20: #1 finished! INFO @ Mon, 29 Jun 2020 22:38:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:38:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:38:20: 13000000 INFO @ Mon, 29 Jun 2020 22:38:21: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 22:38:21: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:38:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:38:26: 14000000 INFO @ Mon, 29 Jun 2020 22:38:31: 15000000 INFO @ Mon, 29 Jun 2020 22:38:37: 16000000 INFO @ Mon, 29 Jun 2020 22:38:42: 17000000 INFO @ Mon, 29 Jun 2020 22:38:47: 18000000 INFO @ Mon, 29 Jun 2020 22:38:53: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:38:57: #1 tag size is determined as 42 bps INFO @ Mon, 29 Jun 2020 22:38:57: #1 tag size = 42 INFO @ Mon, 29 Jun 2020 22:38:57: #1 total tags in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:38:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:38:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:38:58: #1 tags after filtering in treatment: 19828096 INFO @ Mon, 29 Jun 2020 22:38:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:38:58: #1 finished! INFO @ Mon, 29 Jun 2020 22:38:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:38:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:38:59: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 22:38:59: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:38:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245838/SRX245838.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。