Job ID = 1294329 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 38,963,284 reads read : 38,963,284 reads written : 38,963,284 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:34 38963284 reads; of these: 38963284 (100.00%) were unpaired; of these: 1396130 (3.58%) aligned 0 times 30211336 (77.54%) aligned exactly 1 time 7355818 (18.88%) aligned >1 times 96.42% overall alignment rate Time searching: 00:10:34 Overall time: 00:10:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3708340 / 37567154 = 0.0987 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 06:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 06:59:09: #1 read tag files... INFO @ Mon, 03 Jun 2019 06:59:09: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 06:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 06:59:09: #1 read tag files... INFO @ Mon, 03 Jun 2019 06:59:09: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 06:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 06:59:09: #1 read tag files... INFO @ Mon, 03 Jun 2019 06:59:09: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 06:59:16: 1000000 INFO @ Mon, 03 Jun 2019 06:59:17: 1000000 INFO @ Mon, 03 Jun 2019 06:59:17: 1000000 INFO @ Mon, 03 Jun 2019 06:59:23: 2000000 INFO @ Mon, 03 Jun 2019 06:59:24: 2000000 INFO @ Mon, 03 Jun 2019 06:59:25: 2000000 INFO @ Mon, 03 Jun 2019 06:59:30: 3000000 INFO @ Mon, 03 Jun 2019 06:59:31: 3000000 INFO @ Mon, 03 Jun 2019 06:59:32: 3000000 INFO @ Mon, 03 Jun 2019 06:59:37: 4000000 INFO @ Mon, 03 Jun 2019 06:59:39: 4000000 INFO @ Mon, 03 Jun 2019 06:59:40: 4000000 INFO @ Mon, 03 Jun 2019 06:59:44: 5000000 INFO @ Mon, 03 Jun 2019 06:59:46: 5000000 INFO @ Mon, 03 Jun 2019 06:59:48: 5000000 INFO @ Mon, 03 Jun 2019 06:59:51: 6000000 INFO @ Mon, 03 Jun 2019 06:59:53: 6000000 INFO @ Mon, 03 Jun 2019 06:59:56: 6000000 INFO @ Mon, 03 Jun 2019 06:59:59: 7000000 INFO @ Mon, 03 Jun 2019 07:00:01: 7000000 INFO @ Mon, 03 Jun 2019 07:00:03: 7000000 INFO @ Mon, 03 Jun 2019 07:00:05: 8000000 INFO @ Mon, 03 Jun 2019 07:00:08: 8000000 INFO @ Mon, 03 Jun 2019 07:00:11: 8000000 INFO @ Mon, 03 Jun 2019 07:00:12: 9000000 INFO @ Mon, 03 Jun 2019 07:00:15: 9000000 INFO @ Mon, 03 Jun 2019 07:00:18: 9000000 INFO @ Mon, 03 Jun 2019 07:00:19: 10000000 INFO @ Mon, 03 Jun 2019 07:00:23: 10000000 INFO @ Mon, 03 Jun 2019 07:00:26: 10000000 INFO @ Mon, 03 Jun 2019 07:00:27: 11000000 INFO @ Mon, 03 Jun 2019 07:00:30: 11000000 INFO @ Mon, 03 Jun 2019 07:00:34: 11000000 INFO @ Mon, 03 Jun 2019 07:00:35: 12000000 INFO @ Mon, 03 Jun 2019 07:00:37: 12000000 INFO @ Mon, 03 Jun 2019 07:00:41: 12000000 INFO @ Mon, 03 Jun 2019 07:00:42: 13000000 INFO @ Mon, 03 Jun 2019 07:00:44: 13000000 INFO @ Mon, 03 Jun 2019 07:00:49: 13000000 INFO @ Mon, 03 Jun 2019 07:00:49: 14000000 INFO @ Mon, 03 Jun 2019 07:00:51: 14000000 INFO @ Mon, 03 Jun 2019 07:00:56: 15000000 INFO @ Mon, 03 Jun 2019 07:00:57: 14000000 INFO @ Mon, 03 Jun 2019 07:00:58: 15000000 INFO @ Mon, 03 Jun 2019 07:01:04: 16000000 INFO @ Mon, 03 Jun 2019 07:01:04: 15000000 INFO @ Mon, 03 Jun 2019 07:01:05: 16000000 INFO @ Mon, 03 Jun 2019 07:01:11: 17000000 INFO @ Mon, 03 Jun 2019 07:01:12: 17000000 INFO @ Mon, 03 Jun 2019 07:01:12: 16000000 INFO @ Mon, 03 Jun 2019 07:01:18: 18000000 INFO @ Mon, 03 Jun 2019 07:01:19: 18000000 INFO @ Mon, 03 Jun 2019 07:01:19: 17000000 INFO @ Mon, 03 Jun 2019 07:01:26: 19000000 INFO @ Mon, 03 Jun 2019 07:01:26: 19000000 INFO @ Mon, 03 Jun 2019 07:01:27: 18000000 INFO @ Mon, 03 Jun 2019 07:01:33: 20000000 INFO @ Mon, 03 Jun 2019 07:01:33: 20000000 INFO @ Mon, 03 Jun 2019 07:01:34: 19000000 INFO @ Mon, 03 Jun 2019 07:01:40: 21000000 INFO @ Mon, 03 Jun 2019 07:01:40: 21000000 INFO @ Mon, 03 Jun 2019 07:01:42: 20000000 INFO @ Mon, 03 Jun 2019 07:01:47: 22000000 INFO @ Mon, 03 Jun 2019 07:01:47: 22000000 INFO @ Mon, 03 Jun 2019 07:01:50: 21000000 INFO @ Mon, 03 Jun 2019 07:01:53: 23000000 INFO @ Mon, 03 Jun 2019 07:01:54: 23000000 INFO @ Mon, 03 Jun 2019 07:01:58: 22000000 INFO @ Mon, 03 Jun 2019 07:02:00: 24000000 INFO @ Mon, 03 Jun 2019 07:02:02: 24000000 INFO @ Mon, 03 Jun 2019 07:02:05: 23000000 INFO @ Mon, 03 Jun 2019 07:02:07: 25000000 INFO @ Mon, 03 Jun 2019 07:02:09: 25000000 INFO @ Mon, 03 Jun 2019 07:02:13: 24000000 INFO @ Mon, 03 Jun 2019 07:02:14: 26000000 INFO @ Mon, 03 Jun 2019 07:02:16: 26000000 INFO @ Mon, 03 Jun 2019 07:02:21: 25000000 INFO @ Mon, 03 Jun 2019 07:02:21: 27000000 INFO @ Mon, 03 Jun 2019 07:02:23: 27000000 INFO @ Mon, 03 Jun 2019 07:02:28: 28000000 INFO @ Mon, 03 Jun 2019 07:02:29: 26000000 INFO @ Mon, 03 Jun 2019 07:02:30: 28000000 INFO @ Mon, 03 Jun 2019 07:02:35: 29000000 INFO @ Mon, 03 Jun 2019 07:02:36: 27000000 INFO @ Mon, 03 Jun 2019 07:02:38: 29000000 INFO @ Mon, 03 Jun 2019 07:02:42: 30000000 INFO @ Mon, 03 Jun 2019 07:02:44: 28000000 INFO @ Mon, 03 Jun 2019 07:02:45: 30000000 INFO @ Mon, 03 Jun 2019 07:02:49: 31000000 INFO @ Mon, 03 Jun 2019 07:02:52: 31000000 INFO @ Mon, 03 Jun 2019 07:02:52: 29000000 INFO @ Mon, 03 Jun 2019 07:02:56: 32000000 INFO @ Mon, 03 Jun 2019 07:02:59: 32000000 INFO @ Mon, 03 Jun 2019 07:03:00: 30000000 INFO @ Mon, 03 Jun 2019 07:03:03: 33000000 INFO @ Mon, 03 Jun 2019 07:03:06: 33000000 INFO @ Mon, 03 Jun 2019 07:03:08: 31000000 INFO @ Mon, 03 Jun 2019 07:03:09: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 07:03:09: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 07:03:09: #1 total tags in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:03:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:03:10: #1 tags after filtering in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:03:10: #1 finished! INFO @ Mon, 03 Jun 2019 07:03:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:03:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:03:12: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 07:03:12: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:03:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 07:03:13: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 07:03:13: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 07:03:13: #1 total tags in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:03:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:03:13: #1 tags after filtering in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:03:13: #1 finished! INFO @ Mon, 03 Jun 2019 07:03:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:03:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:03:15: 32000000 INFO @ Mon, 03 Jun 2019 07:03:16: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 07:03:16: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:03:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 07:03:23: 33000000 INFO @ Mon, 03 Jun 2019 07:03:30: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 07:03:30: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 07:03:30: #1 total tags in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 07:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 07:03:30: #1 tags after filtering in treatment: 33858814 INFO @ Mon, 03 Jun 2019 07:03:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 07:03:30: #1 finished! INFO @ Mon, 03 Jun 2019 07:03:30: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 07:03:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 07:03:33: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 07:03:33: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 07:03:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX245837/SRX245837.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。