Job ID = 11171153 sra ファイルのダウンロード中... Completed: 1746649K bytes transferred in 20 seconds (713677K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 66927365 spots for /home/okishinya/chipatlas/results/dm3/SRX2325659/SRR4896347.sra Written 66927365 spots for /home/okishinya/chipatlas/results/dm3/SRX2325659/SRR4896347.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:34 66927365 reads; of these: 66927365 (100.00%) were unpaired; of these: 15971013 (23.86%) aligned 0 times 40851146 (61.04%) aligned exactly 1 time 10105206 (15.10%) aligned >1 times 76.14% overall alignment rate Time searching: 00:30:34 Overall time: 00:30:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 18999988 / 50956352 = 0.3729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:57:38: # Command line: callpeak -t SRX2325659.bam -f BAM -g dm -n SRX2325659.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2325659.20 # format = BAM # ChIP-seq file = ['SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:57:38: # Command line: callpeak -t SRX2325659.bam -f BAM -g dm -n SRX2325659.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2325659.05 # format = BAM # ChIP-seq file = ['SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:57:38: # Command line: callpeak -t SRX2325659.bam -f BAM -g dm -n SRX2325659.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2325659.10 # format = BAM # ChIP-seq file = ['SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:57:38: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:57:38: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:57:38: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:57:38: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:57:38: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:57:38: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:57:45: 1000000 INFO @ Sat, 08 Sep 2018 12:57:46: 1000000 INFO @ Sat, 08 Sep 2018 12:57:46: 1000000 INFO @ Sat, 08 Sep 2018 12:57:52: 2000000 INFO @ Sat, 08 Sep 2018 12:57:54: 2000000 INFO @ Sat, 08 Sep 2018 12:57:54: 2000000 INFO @ Sat, 08 Sep 2018 12:57:59: 3000000 INFO @ Sat, 08 Sep 2018 12:58:02: 3000000 INFO @ Sat, 08 Sep 2018 12:58:02: 3000000 INFO @ Sat, 08 Sep 2018 12:58:06: 4000000 INFO @ Sat, 08 Sep 2018 12:58:10: 4000000 INFO @ Sat, 08 Sep 2018 12:58:11: 4000000 INFO @ Sat, 08 Sep 2018 12:58:13: 5000000 INFO @ Sat, 08 Sep 2018 12:58:18: 5000000 INFO @ Sat, 08 Sep 2018 12:58:20: 5000000 INFO @ Sat, 08 Sep 2018 12:58:20: 6000000 INFO @ Sat, 08 Sep 2018 12:58:26: 6000000 INFO @ Sat, 08 Sep 2018 12:58:27: 7000000 INFO @ Sat, 08 Sep 2018 12:58:28: 6000000 INFO @ Sat, 08 Sep 2018 12:58:34: 7000000 INFO @ Sat, 08 Sep 2018 12:58:34: 8000000 INFO @ Sat, 08 Sep 2018 12:58:36: 7000000 INFO @ Sat, 08 Sep 2018 12:58:41: 9000000 INFO @ Sat, 08 Sep 2018 12:58:42: 8000000 INFO @ Sat, 08 Sep 2018 12:58:45: 8000000 INFO @ Sat, 08 Sep 2018 12:58:48: 10000000 INFO @ Sat, 08 Sep 2018 12:58:49: 9000000 INFO @ Sat, 08 Sep 2018 12:58:53: 9000000 INFO @ Sat, 08 Sep 2018 12:58:55: 11000000 INFO @ Sat, 08 Sep 2018 12:58:57: 10000000 INFO @ Sat, 08 Sep 2018 12:59:01: 10000000 INFO @ Sat, 08 Sep 2018 12:59:02: 12000000 INFO @ Sat, 08 Sep 2018 12:59:05: 11000000 INFO @ Sat, 08 Sep 2018 12:59:09: 13000000 INFO @ Sat, 08 Sep 2018 12:59:09: 11000000 INFO @ Sat, 08 Sep 2018 12:59:13: 12000000 INFO @ Sat, 08 Sep 2018 12:59:16: 14000000 INFO @ Sat, 08 Sep 2018 12:59:18: 12000000 INFO @ Sat, 08 Sep 2018 12:59:21: 13000000 INFO @ Sat, 08 Sep 2018 12:59:23: 15000000 INFO @ Sat, 08 Sep 2018 12:59:26: 13000000 INFO @ Sat, 08 Sep 2018 12:59:28: 14000000 INFO @ Sat, 08 Sep 2018 12:59:30: 16000000 INFO @ Sat, 08 Sep 2018 12:59:34: 14000000 INFO @ Sat, 08 Sep 2018 12:59:36: 15000000 INFO @ Sat, 08 Sep 2018 12:59:37: 17000000 INFO @ Sat, 08 Sep 2018 12:59:42: 15000000 INFO @ Sat, 08 Sep 2018 12:59:44: 16000000 INFO @ Sat, 08 Sep 2018 12:59:44: 18000000 INFO @ Sat, 08 Sep 2018 12:59:50: 16000000 INFO @ Sat, 08 Sep 2018 12:59:51: 19000000 INFO @ Sat, 08 Sep 2018 12:59:51: 17000000 INFO @ Sat, 08 Sep 2018 12:59:58: 17000000 INFO @ Sat, 08 Sep 2018 12:59:58: 20000000 INFO @ Sat, 08 Sep 2018 12:59:59: 18000000 INFO @ Sat, 08 Sep 2018 13:00:05: 21000000 INFO @ Sat, 08 Sep 2018 13:00:06: 18000000 INFO @ Sat, 08 Sep 2018 13:00:06: 19000000 INFO @ Sat, 08 Sep 2018 13:00:12: 22000000 INFO @ Sat, 08 Sep 2018 13:00:14: 19000000 INFO @ Sat, 08 Sep 2018 13:00:14: 20000000 INFO @ Sat, 08 Sep 2018 13:00:19: 23000000 INFO @ Sat, 08 Sep 2018 13:00:22: 21000000 INFO @ Sat, 08 Sep 2018 13:00:22: 20000000 INFO @ Sat, 08 Sep 2018 13:00:26: 24000000 INFO @ Sat, 08 Sep 2018 13:00:29: 22000000 INFO @ Sat, 08 Sep 2018 13:00:30: 21000000 INFO @ Sat, 08 Sep 2018 13:00:33: 25000000 INFO @ Sat, 08 Sep 2018 13:00:37: 23000000 INFO @ Sat, 08 Sep 2018 13:00:38: 22000000 INFO @ Sat, 08 Sep 2018 13:00:40: 26000000 INFO @ Sat, 08 Sep 2018 13:00:44: 24000000 INFO @ Sat, 08 Sep 2018 13:00:46: 23000000 INFO @ Sat, 08 Sep 2018 13:00:47: 27000000 INFO @ Sat, 08 Sep 2018 13:00:52: 25000000 INFO @ Sat, 08 Sep 2018 13:00:54: 24000000 INFO @ Sat, 08 Sep 2018 13:00:54: 28000000 INFO @ Sat, 08 Sep 2018 13:01:00: 26000000 INFO @ Sat, 08 Sep 2018 13:01:01: 29000000 INFO @ Sat, 08 Sep 2018 13:01:02: 25000000 INFO @ Sat, 08 Sep 2018 13:01:07: 27000000 INFO @ Sat, 08 Sep 2018 13:01:08: 30000000 INFO @ Sat, 08 Sep 2018 13:01:10: 26000000 INFO @ Sat, 08 Sep 2018 13:01:15: 28000000 INFO @ Sat, 08 Sep 2018 13:01:15: 31000000 INFO @ Sat, 08 Sep 2018 13:01:18: 27000000 INFO @ Sat, 08 Sep 2018 13:01:22: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:01:22: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:01:22: #1 total tags in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:22: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:01:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:01:23: #1 tags after filtering in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:01:23: #1 finished! INFO @ Sat, 08 Sep 2018 13:01:23: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:01:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:01:23: 29000000 INFO @ Sat, 08 Sep 2018 13:01:25: #2 number of paired peaks: 2 WARNING @ Sat, 08 Sep 2018 13:01:25: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:01:25: Process for pairing-model is terminated! cat: SRX2325659.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325659.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:01:26: 28000000 INFO @ Sat, 08 Sep 2018 13:01:31: 30000000 INFO @ Sat, 08 Sep 2018 13:01:35: 29000000 INFO @ Sat, 08 Sep 2018 13:01:39: 31000000 INFO @ Sat, 08 Sep 2018 13:01:44: 30000000 INFO @ Sat, 08 Sep 2018 13:01:47: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:01:47: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:01:47: #1 total tags in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:47: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:01:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:01:48: #1 tags after filtering in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:01:48: #1 finished! INFO @ Sat, 08 Sep 2018 13:01:48: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:01:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:01:50: #2 number of paired peaks: 2 WARNING @ Sat, 08 Sep 2018 13:01:50: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:01:50: Process for pairing-model is terminated! cat: SRX2325659.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 10 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325659.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:01:51: 31000000 INFO @ Sat, 08 Sep 2018 13:01:58: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:01:58: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:01:58: #1 total tags in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:58: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:01:59: #1 tags after filtering in treatment: 31956364 INFO @ Sat, 08 Sep 2018 13:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:01:59: #1 finished! INFO @ Sat, 08 Sep 2018 13:01:59: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:02:01: #2 number of paired peaks: 2 WARNING @ Sat, 08 Sep 2018 13:02:01: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:02:01: Process for pairing-model is terminated! cat: SRX2325659.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325659.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325659.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。