Job ID = 11171150 sra ファイルのダウンロード中... Completed: 1202211K bytes transferred in 14 seconds (672037K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 46993833 spots for /home/okishinya/chipatlas/results/dm3/SRX2325656/SRR4896344.sra Written 46993833 spots for /home/okishinya/chipatlas/results/dm3/SRX2325656/SRR4896344.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:15 46993833 reads; of these: 46993833 (100.00%) were unpaired; of these: 3154315 (6.71%) aligned 0 times 35710906 (75.99%) aligned exactly 1 time 8128612 (17.30%) aligned >1 times 93.29% overall alignment rate Time searching: 00:22:15 Overall time: 00:22:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7612472 / 43839518 = 0.1736 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:43:44: # Command line: callpeak -t SRX2325656.bam -f BAM -g dm -n SRX2325656.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2325656.20 # format = BAM # ChIP-seq file = ['SRX2325656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:43:44: # Command line: callpeak -t SRX2325656.bam -f BAM -g dm -n SRX2325656.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2325656.05 # format = BAM # ChIP-seq file = ['SRX2325656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:43:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:43:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:43:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:43:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:43:44: # Command line: callpeak -t SRX2325656.bam -f BAM -g dm -n SRX2325656.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2325656.10 # format = BAM # ChIP-seq file = ['SRX2325656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:43:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:43:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:43:51: 1000000 INFO @ Sat, 08 Sep 2018 12:43:51: 1000000 INFO @ Sat, 08 Sep 2018 12:43:51: 1000000 INFO @ Sat, 08 Sep 2018 12:43:58: 2000000 INFO @ Sat, 08 Sep 2018 12:43:58: 2000000 INFO @ Sat, 08 Sep 2018 12:43:58: 2000000 INFO @ Sat, 08 Sep 2018 12:44:05: 3000000 INFO @ Sat, 08 Sep 2018 12:44:05: 3000000 INFO @ Sat, 08 Sep 2018 12:44:05: 3000000 INFO @ Sat, 08 Sep 2018 12:44:12: 4000000 INFO @ Sat, 08 Sep 2018 12:44:12: 4000000 INFO @ Sat, 08 Sep 2018 12:44:12: 4000000 INFO @ Sat, 08 Sep 2018 12:44:19: 5000000 INFO @ Sat, 08 Sep 2018 12:44:19: 5000000 INFO @ Sat, 08 Sep 2018 12:44:19: 5000000 INFO @ Sat, 08 Sep 2018 12:44:26: 6000000 INFO @ Sat, 08 Sep 2018 12:44:26: 6000000 INFO @ Sat, 08 Sep 2018 12:44:26: 6000000 INFO @ Sat, 08 Sep 2018 12:44:33: 7000000 INFO @ Sat, 08 Sep 2018 12:44:34: 7000000 INFO @ Sat, 08 Sep 2018 12:44:34: 7000000 INFO @ Sat, 08 Sep 2018 12:44:41: 8000000 INFO @ Sat, 08 Sep 2018 12:44:41: 8000000 INFO @ Sat, 08 Sep 2018 12:44:41: 8000000 INFO @ Sat, 08 Sep 2018 12:44:48: 9000000 INFO @ Sat, 08 Sep 2018 12:44:48: 9000000 INFO @ Sat, 08 Sep 2018 12:44:48: 9000000 INFO @ Sat, 08 Sep 2018 12:44:55: 10000000 INFO @ Sat, 08 Sep 2018 12:44:55: 10000000 INFO @ Sat, 08 Sep 2018 12:44:56: 10000000 INFO @ Sat, 08 Sep 2018 12:45:03: 11000000 INFO @ Sat, 08 Sep 2018 12:45:03: 11000000 INFO @ Sat, 08 Sep 2018 12:45:04: 11000000 INFO @ Sat, 08 Sep 2018 12:45:10: 12000000 INFO @ Sat, 08 Sep 2018 12:45:10: 12000000 INFO @ Sat, 08 Sep 2018 12:45:12: 12000000 INFO @ Sat, 08 Sep 2018 12:45:18: 13000000 INFO @ Sat, 08 Sep 2018 12:45:18: 13000000 INFO @ Sat, 08 Sep 2018 12:45:20: 13000000 INFO @ Sat, 08 Sep 2018 12:45:25: 14000000 INFO @ Sat, 08 Sep 2018 12:45:25: 14000000 INFO @ Sat, 08 Sep 2018 12:45:27: 14000000 INFO @ Sat, 08 Sep 2018 12:45:33: 15000000 INFO @ Sat, 08 Sep 2018 12:45:33: 15000000 INFO @ Sat, 08 Sep 2018 12:45:35: 15000000 INFO @ Sat, 08 Sep 2018 12:45:40: 16000000 INFO @ Sat, 08 Sep 2018 12:45:40: 16000000 INFO @ Sat, 08 Sep 2018 12:45:43: 16000000 INFO @ Sat, 08 Sep 2018 12:45:48: 17000000 INFO @ Sat, 08 Sep 2018 12:45:48: 17000000 INFO @ Sat, 08 Sep 2018 12:45:51: 17000000 INFO @ Sat, 08 Sep 2018 12:45:55: 18000000 INFO @ Sat, 08 Sep 2018 12:45:55: 18000000 INFO @ Sat, 08 Sep 2018 12:45:59: 18000000 INFO @ Sat, 08 Sep 2018 12:46:02: 19000000 INFO @ Sat, 08 Sep 2018 12:46:02: 19000000 INFO @ Sat, 08 Sep 2018 12:46:06: 19000000 INFO @ Sat, 08 Sep 2018 12:46:10: 20000000 INFO @ Sat, 08 Sep 2018 12:46:10: 20000000 INFO @ Sat, 08 Sep 2018 12:46:14: 20000000 INFO @ Sat, 08 Sep 2018 12:46:17: 21000000 INFO @ Sat, 08 Sep 2018 12:46:17: 21000000 INFO @ Sat, 08 Sep 2018 12:46:22: 21000000 INFO @ Sat, 08 Sep 2018 12:46:24: 22000000 INFO @ Sat, 08 Sep 2018 12:46:24: 22000000 INFO @ Sat, 08 Sep 2018 12:46:29: 22000000 INFO @ Sat, 08 Sep 2018 12:46:32: 23000000 INFO @ Sat, 08 Sep 2018 12:46:32: 23000000 INFO @ Sat, 08 Sep 2018 12:46:37: 23000000 INFO @ Sat, 08 Sep 2018 12:46:39: 24000000 INFO @ Sat, 08 Sep 2018 12:46:39: 24000000 INFO @ Sat, 08 Sep 2018 12:46:45: 24000000 INFO @ Sat, 08 Sep 2018 12:46:47: 25000000 INFO @ Sat, 08 Sep 2018 12:46:47: 25000000 INFO @ Sat, 08 Sep 2018 12:46:52: 25000000 INFO @ Sat, 08 Sep 2018 12:46:54: 26000000 INFO @ Sat, 08 Sep 2018 12:46:54: 26000000 INFO @ Sat, 08 Sep 2018 12:47:00: 26000000 INFO @ Sat, 08 Sep 2018 12:47:01: 27000000 INFO @ Sat, 08 Sep 2018 12:47:01: 27000000 INFO @ Sat, 08 Sep 2018 12:47:08: 27000000 INFO @ Sat, 08 Sep 2018 12:47:09: 28000000 INFO @ Sat, 08 Sep 2018 12:47:09: 28000000 INFO @ Sat, 08 Sep 2018 12:47:15: 28000000 INFO @ Sat, 08 Sep 2018 12:47:16: 29000000 INFO @ Sat, 08 Sep 2018 12:47:16: 29000000 INFO @ Sat, 08 Sep 2018 12:47:23: 29000000 INFO @ Sat, 08 Sep 2018 12:47:24: 30000000 INFO @ Sat, 08 Sep 2018 12:47:24: 30000000 INFO @ Sat, 08 Sep 2018 12:47:31: 30000000 INFO @ Sat, 08 Sep 2018 12:47:31: 31000000 INFO @ Sat, 08 Sep 2018 12:47:31: 31000000 INFO @ Sat, 08 Sep 2018 12:47:39: 32000000 INFO @ Sat, 08 Sep 2018 12:47:39: 32000000 INFO @ Sat, 08 Sep 2018 12:47:39: 31000000 INFO @ Sat, 08 Sep 2018 12:47:46: 33000000 INFO @ Sat, 08 Sep 2018 12:47:46: 33000000 INFO @ Sat, 08 Sep 2018 12:47:46: 32000000 INFO @ Sat, 08 Sep 2018 12:47:54: 34000000 INFO @ Sat, 08 Sep 2018 12:47:54: 34000000 INFO @ Sat, 08 Sep 2018 12:47:54: 33000000 INFO @ Sat, 08 Sep 2018 12:48:01: 35000000 INFO @ Sat, 08 Sep 2018 12:48:01: 35000000 INFO @ Sat, 08 Sep 2018 12:48:02: 34000000 INFO @ Sat, 08 Sep 2018 12:48:08: 36000000 INFO @ Sat, 08 Sep 2018 12:48:08: 36000000 INFO @ Sat, 08 Sep 2018 12:48:10: 35000000 INFO @ Sat, 08 Sep 2018 12:48:10: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:48:10: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:48:10: #1 total tags in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:48:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:48:10: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:48:10: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:48:10: #1 total tags in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:48:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:48:11: #1 tags after filtering in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:48:11: #1 finished! INFO @ Sat, 08 Sep 2018 12:48:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:48:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:48:11: #1 tags after filtering in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:48:11: #1 finished! INFO @ Sat, 08 Sep 2018 12:48:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:48:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:48:13: #2 number of paired peaks: 23 WARNING @ Sat, 08 Sep 2018 12:48:13: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:48:13: Process for pairing-model is terminated! cat: SRX2325656.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325656.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:48:13: #2 number of paired peaks: 23 WARNING @ Sat, 08 Sep 2018 12:48:13: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:48:13: Process for pairing-model is terminated! cat: SRX2325656.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325656.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:48:16: 36000000 INFO @ Sat, 08 Sep 2018 12:48:18: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:48:18: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:48:18: #1 total tags in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:18: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:48:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:48:19: #1 tags after filtering in treatment: 36227046 INFO @ Sat, 08 Sep 2018 12:48:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:48:19: #1 finished! INFO @ Sat, 08 Sep 2018 12:48:19: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:48:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:48:22: #2 number of paired peaks: 23 WARNING @ Sat, 08 Sep 2018 12:48:22: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:48:22: Process for pairing-model is terminated! cat: SRX2325656.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325656.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325656.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。