Job ID = 6527677 SRX = SRX202835 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:13:03 prefetch.2.10.7: 1) Downloading 'SRR611196'... 2020-06-29T13:13:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:14:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:14:37 prefetch.2.10.7: 'SRR611196' is valid 2020-06-29T13:14:37 prefetch.2.10.7: 1) 'SRR611196' was downloaded successfully Read 7449983 spots for SRR611196/SRR611196.sra Written 7449983 spots for SRR611196/SRR611196.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:38 7449983 reads; of these: 7449983 (100.00%) were unpaired; of these: 183306 (2.46%) aligned 0 times 6188974 (83.07%) aligned exactly 1 time 1077703 (14.47%) aligned >1 times 97.54% overall alignment rate Time searching: 00:02:38 Overall time: 00:02:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 384768 / 7266677 = 0.0529 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:23:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:23:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:23:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:23:12: 1000000 INFO @ Mon, 29 Jun 2020 22:23:18: 2000000 INFO @ Mon, 29 Jun 2020 22:23:24: 3000000 INFO @ Mon, 29 Jun 2020 22:23:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:23:36: 5000000 INFO @ Mon, 29 Jun 2020 22:23:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:23:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:23:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:23:42: 6000000 INFO @ Mon, 29 Jun 2020 22:23:43: 1000000 INFO @ Mon, 29 Jun 2020 22:23:48: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:23:48: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:23:48: #1 total tags in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:23:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:23:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:23:48: #1 tags after filtering in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:23:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:23:48: #1 finished! INFO @ Mon, 29 Jun 2020 22:23:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:23:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:23:49: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:23:49: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:23:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:23:49: 2000000 INFO @ Mon, 29 Jun 2020 22:23:56: 3000000 INFO @ Mon, 29 Jun 2020 22:24:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:24:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:24:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:24:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:24:08: 5000000 INFO @ Mon, 29 Jun 2020 22:24:13: 1000000 INFO @ Mon, 29 Jun 2020 22:24:15: 6000000 INFO @ Mon, 29 Jun 2020 22:24:19: 2000000 INFO @ Mon, 29 Jun 2020 22:24:21: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:24:21: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:24:21: #1 total tags in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:24:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:24:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:24:21: #1 tags after filtering in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:24:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:24:21: #1 finished! INFO @ Mon, 29 Jun 2020 22:24:21: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:24:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:24:21: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:24:21: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:24:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:24:26: 3000000 INFO @ Mon, 29 Jun 2020 22:24:32: 4000000 INFO @ Mon, 29 Jun 2020 22:24:38: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:24:44: 6000000 INFO @ Mon, 29 Jun 2020 22:24:50: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:24:50: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:24:50: #1 total tags in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:24:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:24:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:24:50: #1 tags after filtering in treatment: 6881909 INFO @ Mon, 29 Jun 2020 22:24:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:24:50: #1 finished! INFO @ Mon, 29 Jun 2020 22:24:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:24:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:24:50: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:24:50: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:24:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202835/SRX202835.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。