Job ID = 6527676 SRX = SRX202834 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:17:55 prefetch.2.10.7: 1) Downloading 'SRR611195'... 2020-06-29T13:17:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:19:42 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:19:42 prefetch.2.10.7: 1) 'SRR611195' was downloaded successfully Read 12145465 spots for SRR611195/SRR611195.sra Written 12145465 spots for SRR611195/SRR611195.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:25 12145465 reads; of these: 12145465 (100.00%) were unpaired; of these: 301582 (2.48%) aligned 0 times 9550034 (78.63%) aligned exactly 1 time 2293849 (18.89%) aligned >1 times 97.52% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1437900 / 11843883 = 0.1214 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:35:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:35:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:35:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:35:36: 1000000 INFO @ Mon, 29 Jun 2020 22:35:43: 2000000 INFO @ Mon, 29 Jun 2020 22:35:50: 3000000 INFO @ Mon, 29 Jun 2020 22:35:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:35:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:35:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:35:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:36:05: 5000000 INFO @ Mon, 29 Jun 2020 22:36:08: 1000000 INFO @ Mon, 29 Jun 2020 22:36:13: 6000000 INFO @ Mon, 29 Jun 2020 22:36:16: 2000000 INFO @ Mon, 29 Jun 2020 22:36:21: 7000000 INFO @ Mon, 29 Jun 2020 22:36:23: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:36:29: 8000000 INFO @ Mon, 29 Jun 2020 22:36:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:36:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:36:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:36:32: 4000000 INFO @ Mon, 29 Jun 2020 22:36:37: 9000000 INFO @ Mon, 29 Jun 2020 22:36:38: 1000000 INFO @ Mon, 29 Jun 2020 22:36:40: 5000000 INFO @ Mon, 29 Jun 2020 22:36:45: 10000000 INFO @ Mon, 29 Jun 2020 22:36:46: 2000000 INFO @ Mon, 29 Jun 2020 22:36:48: 6000000 INFO @ Mon, 29 Jun 2020 22:36:48: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:36:48: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:36:48: #1 total tags in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:36:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:36:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:36:49: #1 tags after filtering in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:36:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:36:49: #1 finished! INFO @ Mon, 29 Jun 2020 22:36:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:36:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:36:49: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:36:49: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:36:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:36:54: 3000000 INFO @ Mon, 29 Jun 2020 22:36:56: 7000000 INFO @ Mon, 29 Jun 2020 22:37:02: 4000000 INFO @ Mon, 29 Jun 2020 22:37:03: 8000000 INFO @ Mon, 29 Jun 2020 22:37:10: 5000000 INFO @ Mon, 29 Jun 2020 22:37:11: 9000000 INFO @ Mon, 29 Jun 2020 22:37:18: 6000000 INFO @ Mon, 29 Jun 2020 22:37:20: 10000000 INFO @ Mon, 29 Jun 2020 22:37:23: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:37:23: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:37:23: #1 total tags in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:37:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:37:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:37:23: #1 tags after filtering in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:37:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:37:23: #1 finished! INFO @ Mon, 29 Jun 2020 22:37:23: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:37:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:37:24: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:37:24: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:37:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:37:26: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:37:33: 8000000 INFO @ Mon, 29 Jun 2020 22:37:41: 9000000 INFO @ Mon, 29 Jun 2020 22:37:48: 10000000 INFO @ Mon, 29 Jun 2020 22:37:52: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:37:52: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:37:52: #1 total tags in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:37:52: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:37:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:37:52: #1 tags after filtering in treatment: 10405983 INFO @ Mon, 29 Jun 2020 22:37:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:37:52: #1 finished! INFO @ Mon, 29 Jun 2020 22:37:52: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:37:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:37:52: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:37:52: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202834/SRX202834.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。