Job ID = 1294232 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,626,062 reads read : 12,626,062 reads written : 12,626,062 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 12626062 reads; of these: 12626062 (100.00%) were unpaired; of these: 7176433 (56.84%) aligned 0 times 3862279 (30.59%) aligned exactly 1 time 1587350 (12.57%) aligned >1 times 43.16% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2168662 / 5449629 = 0.3979 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:46:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:46:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:46:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:46:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:46:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:46:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:46:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:46:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:46:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:46:41: 1000000 INFO @ Mon, 03 Jun 2019 05:46:41: 1000000 INFO @ Mon, 03 Jun 2019 05:46:43: 1000000 INFO @ Mon, 03 Jun 2019 05:46:48: 2000000 INFO @ Mon, 03 Jun 2019 05:46:48: 2000000 INFO @ Mon, 03 Jun 2019 05:46:52: 2000000 INFO @ Mon, 03 Jun 2019 05:46:55: 3000000 INFO @ Mon, 03 Jun 2019 05:46:55: 3000000 INFO @ Mon, 03 Jun 2019 05:46:57: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 05:46:57: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 05:46:57: #1 total tags in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:46:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:46:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:46:57: #1 tags after filtering in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:46:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:46:57: #1 finished! INFO @ Mon, 03 Jun 2019 05:46:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:46:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:46:57: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 05:46:57: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 05:46:57: #1 total tags in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:46:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:46:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:46:58: #1 tags after filtering in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:46:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:46:58: #1 finished! INFO @ Mon, 03 Jun 2019 05:46:58: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:46:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:46:58: #2 number of paired peaks: 460 WARNING @ Mon, 03 Jun 2019 05:46:58: Fewer paired peaks (460) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 460 pairs to build model! INFO @ Mon, 03 Jun 2019 05:46:58: start model_add_line... INFO @ Mon, 03 Jun 2019 05:46:58: start X-correlation... INFO @ Mon, 03 Jun 2019 05:46:58: end of X-cor INFO @ Mon, 03 Jun 2019 05:46:58: #2 finished! INFO @ Mon, 03 Jun 2019 05:46:58: #2 predicted fragment length is 66 bps INFO @ Mon, 03 Jun 2019 05:46:58: #2 alternative fragment length(s) may be 66,91,499,587 bps INFO @ Mon, 03 Jun 2019 05:46:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20_model.r WARNING @ Mon, 03 Jun 2019 05:46:58: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:46:58: #2 You may need to consider one of the other alternative d(s): 66,91,499,587 WARNING @ Mon, 03 Jun 2019 05:46:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:46:58: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:46:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:46:58: #2 number of paired peaks: 460 WARNING @ Mon, 03 Jun 2019 05:46:58: Fewer paired peaks (460) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 460 pairs to build model! INFO @ Mon, 03 Jun 2019 05:46:58: start model_add_line... INFO @ Mon, 03 Jun 2019 05:46:58: start X-correlation... INFO @ Mon, 03 Jun 2019 05:46:58: end of X-cor INFO @ Mon, 03 Jun 2019 05:46:58: #2 finished! INFO @ Mon, 03 Jun 2019 05:46:58: #2 predicted fragment length is 66 bps INFO @ Mon, 03 Jun 2019 05:46:58: #2 alternative fragment length(s) may be 66,91,499,587 bps INFO @ Mon, 03 Jun 2019 05:46:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05_model.r WARNING @ Mon, 03 Jun 2019 05:46:58: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:46:58: #2 You may need to consider one of the other alternative d(s): 66,91,499,587 WARNING @ Mon, 03 Jun 2019 05:46:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:46:58: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:46:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:47:01: 3000000 INFO @ Mon, 03 Jun 2019 05:47:03: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 05:47:03: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 05:47:03: #1 total tags in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:47:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:47:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:47:03: #1 tags after filtering in treatment: 3280967 INFO @ Mon, 03 Jun 2019 05:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:47:03: #1 finished! INFO @ Mon, 03 Jun 2019 05:47:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:47:03: #2 number of paired peaks: 460 WARNING @ Mon, 03 Jun 2019 05:47:03: Fewer paired peaks (460) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 460 pairs to build model! INFO @ Mon, 03 Jun 2019 05:47:03: start model_add_line... INFO @ Mon, 03 Jun 2019 05:47:03: start X-correlation... INFO @ Mon, 03 Jun 2019 05:47:03: end of X-cor INFO @ Mon, 03 Jun 2019 05:47:03: #2 finished! INFO @ Mon, 03 Jun 2019 05:47:03: #2 predicted fragment length is 66 bps INFO @ Mon, 03 Jun 2019 05:47:03: #2 alternative fragment length(s) may be 66,91,499,587 bps INFO @ Mon, 03 Jun 2019 05:47:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10_model.r WARNING @ Mon, 03 Jun 2019 05:47:03: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:47:03: #2 You may need to consider one of the other alternative d(s): 66,91,499,587 WARNING @ Mon, 03 Jun 2019 05:47:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:47:03: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:47:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:47:07: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:47:08: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:47:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20_peaks.xls INFO @ Mon, 03 Jun 2019 05:47:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:47:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.20_summits.bed INFO @ Mon, 03 Jun 2019 05:47:12: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (129 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:47:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05_peaks.xls INFO @ Mon, 03 Jun 2019 05:47:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:47:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.05_summits.bed INFO @ Mon, 03 Jun 2019 05:47:13: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1219 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:47:13: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:47:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10_peaks.xls INFO @ Mon, 03 Jun 2019 05:47:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:47:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX202121/SRX202121.10_summits.bed INFO @ Mon, 03 Jun 2019 05:47:18: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (389 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。