Job ID = 10320326 sra ファイルのダウンロード中... Completed: 1074336K bytes transferred in 15 seconds (552628K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 35931507 spots for /home/okishinya/chipatlas/results/dm3/SRX2010896/SRR4017019.sra Written 35931507 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:04 35931507 reads; of these: 35931507 (100.00%) were unpaired; of these: 900928 (2.51%) aligned 0 times 24328965 (67.71%) aligned exactly 1 time 10701614 (29.78%) aligned >1 times 97.49% overall alignment rate Time searching: 00:15:05 Overall time: 00:15:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5727945 / 35030579 = 0.1635 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 11 Jan 2018 02:30:28: # Command line: callpeak -t SRX2010896.bam -f BAM -g dm -n SRX2010896.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2010896.10 # format = BAM # ChIP-seq file = ['SRX2010896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:30:28: # Command line: callpeak -t SRX2010896.bam -f BAM -g dm -n SRX2010896.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2010896.20 # format = BAM # ChIP-seq file = ['SRX2010896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:30:28: # Command line: callpeak -t SRX2010896.bam -f BAM -g dm -n SRX2010896.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2010896.05 # format = BAM # ChIP-seq file = ['SRX2010896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:30:28: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:30:28: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:30:28: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:30:28: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:30:28: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:30:28: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:30:35: 1000000 INFO @ Thu, 11 Jan 2018 02:30:35: 1000000 INFO @ Thu, 11 Jan 2018 02:30:35: 1000000 INFO @ Thu, 11 Jan 2018 02:30:41: 2000000 INFO @ Thu, 11 Jan 2018 02:30:41: 2000000 INFO @ Thu, 11 Jan 2018 02:30:42: 2000000 INFO @ Thu, 11 Jan 2018 02:30:48: 3000000 INFO @ Thu, 11 Jan 2018 02:30:48: 3000000 INFO @ Thu, 11 Jan 2018 02:30:49: 3000000 INFO @ Thu, 11 Jan 2018 02:30:55: 4000000 INFO @ Thu, 11 Jan 2018 02:30:55: 4000000 INFO @ Thu, 11 Jan 2018 02:30:55: 4000000 INFO @ Thu, 11 Jan 2018 02:31:02: 5000000 INFO @ Thu, 11 Jan 2018 02:31:02: 5000000 INFO @ Thu, 11 Jan 2018 02:31:02: 5000000 INFO @ Thu, 11 Jan 2018 02:31:08: 6000000 INFO @ Thu, 11 Jan 2018 02:31:09: 6000000 INFO @ Thu, 11 Jan 2018 02:31:09: 6000000 INFO @ Thu, 11 Jan 2018 02:31:15: 7000000 INFO @ Thu, 11 Jan 2018 02:31:16: 7000000 INFO @ Thu, 11 Jan 2018 02:31:16: 7000000 INFO @ Thu, 11 Jan 2018 02:31:21: 8000000 INFO @ Thu, 11 Jan 2018 02:31:22: 8000000 INFO @ Thu, 11 Jan 2018 02:31:23: 8000000 INFO @ Thu, 11 Jan 2018 02:31:28: 9000000 INFO @ Thu, 11 Jan 2018 02:31:29: 9000000 INFO @ Thu, 11 Jan 2018 02:31:30: 9000000 INFO @ Thu, 11 Jan 2018 02:31:35: 10000000 INFO @ Thu, 11 Jan 2018 02:31:36: 10000000 INFO @ Thu, 11 Jan 2018 02:31:36: 10000000 INFO @ Thu, 11 Jan 2018 02:31:41: 11000000 INFO @ Thu, 11 Jan 2018 02:31:43: 11000000 INFO @ Thu, 11 Jan 2018 02:31:43: 11000000 INFO @ Thu, 11 Jan 2018 02:31:48: 12000000 INFO @ Thu, 11 Jan 2018 02:31:50: 12000000 INFO @ Thu, 11 Jan 2018 02:31:50: 12000000 INFO @ Thu, 11 Jan 2018 02:31:54: 13000000 INFO @ Thu, 11 Jan 2018 02:31:56: 13000000 INFO @ Thu, 11 Jan 2018 02:31:57: 13000000 INFO @ Thu, 11 Jan 2018 02:32:01: 14000000 INFO @ Thu, 11 Jan 2018 02:32:03: 14000000 INFO @ Thu, 11 Jan 2018 02:32:04: 14000000 INFO @ Thu, 11 Jan 2018 02:32:08: 15000000 INFO @ Thu, 11 Jan 2018 02:32:10: 15000000 INFO @ Thu, 11 Jan 2018 02:32:10: 15000000 INFO @ Thu, 11 Jan 2018 02:32:14: 16000000 INFO @ Thu, 11 Jan 2018 02:32:17: 16000000 INFO @ Thu, 11 Jan 2018 02:32:17: 16000000 INFO @ Thu, 11 Jan 2018 02:32:21: 17000000 INFO @ Thu, 11 Jan 2018 02:32:24: 17000000 INFO @ Thu, 11 Jan 2018 02:32:24: 17000000 INFO @ Thu, 11 Jan 2018 02:32:27: 18000000 INFO @ Thu, 11 Jan 2018 02:32:30: 18000000 INFO @ Thu, 11 Jan 2018 02:32:31: 18000000 INFO @ Thu, 11 Jan 2018 02:32:34: 19000000 INFO @ Thu, 11 Jan 2018 02:32:37: 19000000 INFO @ Thu, 11 Jan 2018 02:32:38: 19000000 INFO @ Thu, 11 Jan 2018 02:32:41: 20000000 INFO @ Thu, 11 Jan 2018 02:32:44: 20000000 INFO @ Thu, 11 Jan 2018 02:32:44: 20000000 INFO @ Thu, 11 Jan 2018 02:32:47: 21000000 INFO @ Thu, 11 Jan 2018 02:32:51: 21000000 INFO @ Thu, 11 Jan 2018 02:32:51: 21000000 INFO @ Thu, 11 Jan 2018 02:32:54: 22000000 INFO @ Thu, 11 Jan 2018 02:32:58: 22000000 INFO @ Thu, 11 Jan 2018 02:32:58: 22000000 INFO @ Thu, 11 Jan 2018 02:33:01: 23000000 INFO @ Thu, 11 Jan 2018 02:33:05: 23000000 INFO @ Thu, 11 Jan 2018 02:33:05: 23000000 INFO @ Thu, 11 Jan 2018 02:33:07: 24000000 INFO @ Thu, 11 Jan 2018 02:33:12: 24000000 INFO @ Thu, 11 Jan 2018 02:33:12: 24000000 INFO @ Thu, 11 Jan 2018 02:33:14: 25000000 INFO @ Thu, 11 Jan 2018 02:33:19: 25000000 INFO @ Thu, 11 Jan 2018 02:33:19: 25000000 INFO @ Thu, 11 Jan 2018 02:33:21: 26000000 INFO @ Thu, 11 Jan 2018 02:33:25: 26000000 INFO @ Thu, 11 Jan 2018 02:33:26: 26000000 INFO @ Thu, 11 Jan 2018 02:33:27: 27000000 INFO @ Thu, 11 Jan 2018 02:33:32: 27000000 INFO @ Thu, 11 Jan 2018 02:33:33: 27000000 INFO @ Thu, 11 Jan 2018 02:33:34: 28000000 INFO @ Thu, 11 Jan 2018 02:33:39: 28000000 INFO @ Thu, 11 Jan 2018 02:33:40: 28000000 INFO @ Thu, 11 Jan 2018 02:33:40: 29000000 INFO @ Thu, 11 Jan 2018 02:33:43: #1 tag size is determined as 50 bps INFO @ Thu, 11 Jan 2018 02:33:43: #1 tag size = 50 INFO @ Thu, 11 Jan 2018 02:33:43: #1 total tags in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:43: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:33:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:33:43: #1 tags after filtering in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:43: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:33:43: #1 finished! INFO @ Thu, 11 Jan 2018 02:33:43: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:33:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:33:45: #2 number of paired peaks: 67 WARNING @ Thu, 11 Jan 2018 02:33:45: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:33:45: Process for pairing-model is terminated! cat: SRX2010896.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2010896.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:33:46: 29000000 INFO @ Thu, 11 Jan 2018 02:33:47: 29000000 INFO @ Thu, 11 Jan 2018 02:33:48: #1 tag size is determined as 50 bps INFO @ Thu, 11 Jan 2018 02:33:48: #1 tag size = 50 INFO @ Thu, 11 Jan 2018 02:33:48: #1 total tags in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:48: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:33:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:33:49: #1 tags after filtering in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:33:49: #1 finished! INFO @ Thu, 11 Jan 2018 02:33:49: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:33:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:33:49: #1 tag size is determined as 50 bps INFO @ Thu, 11 Jan 2018 02:33:49: #1 tag size = 50 INFO @ Thu, 11 Jan 2018 02:33:49: #1 total tags in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:49: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:33:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:33:49: #1 tags after filtering in treatment: 29302634 INFO @ Thu, 11 Jan 2018 02:33:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:33:49: #1 finished! INFO @ Thu, 11 Jan 2018 02:33:49: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:33:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:33:50: #2 number of paired peaks: 67 WARNING @ Thu, 11 Jan 2018 02:33:50: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:33:50: Process for pairing-model is terminated! cat: SRX2010896.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2010896.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:33:51: #2 number of paired peaks: 67 WARNING @ Thu, 11 Jan 2018 02:33:51: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:33:51: Process for pairing-model is terminated! cat: SRX2010896.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2010896.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2010896.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。