Job ID = 1294201 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 42,007,865 reads read : 42,007,865 reads written : 42,007,865 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:08 42007865 reads; of these: 42007865 (100.00%) were unpaired; of these: 1314146 (3.13%) aligned 0 times 28754292 (68.45%) aligned exactly 1 time 11939427 (28.42%) aligned >1 times 96.87% overall alignment rate Time searching: 00:16:08 Overall time: 00:16:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4338708 / 40693719 = 0.1066 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:57:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:57:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:57:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:57:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:57:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:57:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:57:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:57:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:57:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:57:34: 1000000 INFO @ Mon, 03 Jun 2019 05:57:34: 1000000 INFO @ Mon, 03 Jun 2019 05:57:34: 1000000 INFO @ Mon, 03 Jun 2019 05:57:41: 2000000 INFO @ Mon, 03 Jun 2019 05:57:42: 2000000 INFO @ Mon, 03 Jun 2019 05:57:42: 2000000 INFO @ Mon, 03 Jun 2019 05:57:48: 3000000 INFO @ Mon, 03 Jun 2019 05:57:50: 3000000 INFO @ Mon, 03 Jun 2019 05:57:50: 3000000 INFO @ Mon, 03 Jun 2019 05:57:55: 4000000 INFO @ Mon, 03 Jun 2019 05:57:58: 4000000 INFO @ Mon, 03 Jun 2019 05:57:58: 4000000 INFO @ Mon, 03 Jun 2019 05:58:02: 5000000 INFO @ Mon, 03 Jun 2019 05:58:05: 5000000 INFO @ Mon, 03 Jun 2019 05:58:05: 5000000 INFO @ Mon, 03 Jun 2019 05:58:09: 6000000 INFO @ Mon, 03 Jun 2019 05:58:13: 6000000 INFO @ Mon, 03 Jun 2019 05:58:13: 6000000 INFO @ Mon, 03 Jun 2019 05:58:17: 7000000 INFO @ Mon, 03 Jun 2019 05:58:21: 7000000 INFO @ Mon, 03 Jun 2019 05:58:21: 7000000 INFO @ Mon, 03 Jun 2019 05:58:24: 8000000 INFO @ Mon, 03 Jun 2019 05:58:29: 8000000 INFO @ Mon, 03 Jun 2019 05:58:29: 8000000 INFO @ Mon, 03 Jun 2019 05:58:31: 9000000 INFO @ Mon, 03 Jun 2019 05:58:37: 9000000 INFO @ Mon, 03 Jun 2019 05:58:37: 9000000 INFO @ Mon, 03 Jun 2019 05:58:38: 10000000 INFO @ Mon, 03 Jun 2019 05:58:44: 10000000 INFO @ Mon, 03 Jun 2019 05:58:44: 10000000 INFO @ Mon, 03 Jun 2019 05:58:45: 11000000 INFO @ Mon, 03 Jun 2019 05:58:52: 12000000 INFO @ Mon, 03 Jun 2019 05:58:52: 11000000 INFO @ Mon, 03 Jun 2019 05:58:52: 11000000 INFO @ Mon, 03 Jun 2019 05:58:59: 13000000 INFO @ Mon, 03 Jun 2019 05:59:00: 12000000 INFO @ Mon, 03 Jun 2019 05:59:00: 12000000 INFO @ Mon, 03 Jun 2019 05:59:06: 14000000 INFO @ Mon, 03 Jun 2019 05:59:07: 13000000 INFO @ Mon, 03 Jun 2019 05:59:08: 13000000 INFO @ Mon, 03 Jun 2019 05:59:13: 15000000 INFO @ Mon, 03 Jun 2019 05:59:15: 14000000 INFO @ Mon, 03 Jun 2019 05:59:15: 14000000 INFO @ Mon, 03 Jun 2019 05:59:20: 16000000 INFO @ Mon, 03 Jun 2019 05:59:23: 15000000 INFO @ Mon, 03 Jun 2019 05:59:23: 15000000 INFO @ Mon, 03 Jun 2019 05:59:27: 17000000 INFO @ Mon, 03 Jun 2019 05:59:31: 16000000 INFO @ Mon, 03 Jun 2019 05:59:31: 16000000 INFO @ Mon, 03 Jun 2019 05:59:34: 18000000 INFO @ Mon, 03 Jun 2019 05:59:38: 17000000 INFO @ Mon, 03 Jun 2019 05:59:39: 17000000 INFO @ Mon, 03 Jun 2019 05:59:41: 19000000 INFO @ Mon, 03 Jun 2019 05:59:46: 18000000 INFO @ Mon, 03 Jun 2019 05:59:46: 18000000 INFO @ Mon, 03 Jun 2019 05:59:49: 20000000 INFO @ Mon, 03 Jun 2019 05:59:53: 19000000 INFO @ Mon, 03 Jun 2019 05:59:54: 19000000 INFO @ Mon, 03 Jun 2019 05:59:56: 21000000 INFO @ Mon, 03 Jun 2019 06:00:00: 20000000 INFO @ Mon, 03 Jun 2019 06:00:02: 20000000 INFO @ Mon, 03 Jun 2019 06:00:04: 22000000 INFO @ Mon, 03 Jun 2019 06:00:07: 21000000 INFO @ Mon, 03 Jun 2019 06:00:09: 21000000 INFO @ Mon, 03 Jun 2019 06:00:12: 23000000 INFO @ Mon, 03 Jun 2019 06:00:14: 22000000 INFO @ Mon, 03 Jun 2019 06:00:17: 22000000 INFO @ Mon, 03 Jun 2019 06:00:20: 24000000 INFO @ Mon, 03 Jun 2019 06:00:21: 23000000 INFO @ Mon, 03 Jun 2019 06:00:25: 23000000 INFO @ Mon, 03 Jun 2019 06:00:27: 25000000 INFO @ Mon, 03 Jun 2019 06:00:28: 24000000 INFO @ Mon, 03 Jun 2019 06:00:32: 24000000 INFO @ Mon, 03 Jun 2019 06:00:35: 26000000 INFO @ Mon, 03 Jun 2019 06:00:35: 25000000 INFO @ Mon, 03 Jun 2019 06:00:40: 25000000 INFO @ Mon, 03 Jun 2019 06:00:42: 26000000 INFO @ Mon, 03 Jun 2019 06:00:43: 27000000 INFO @ Mon, 03 Jun 2019 06:00:48: 26000000 INFO @ Mon, 03 Jun 2019 06:00:49: 27000000 INFO @ Mon, 03 Jun 2019 06:00:50: 28000000 INFO @ Mon, 03 Jun 2019 06:00:56: 27000000 INFO @ Mon, 03 Jun 2019 06:00:56: 28000000 INFO @ Mon, 03 Jun 2019 06:00:58: 29000000 INFO @ Mon, 03 Jun 2019 06:01:03: 29000000 INFO @ Mon, 03 Jun 2019 06:01:04: 28000000 INFO @ Mon, 03 Jun 2019 06:01:06: 30000000 INFO @ Mon, 03 Jun 2019 06:01:10: 30000000 INFO @ Mon, 03 Jun 2019 06:01:11: 29000000 INFO @ Mon, 03 Jun 2019 06:01:14: 31000000 INFO @ Mon, 03 Jun 2019 06:01:17: 31000000 INFO @ Mon, 03 Jun 2019 06:01:18: 30000000 INFO @ Mon, 03 Jun 2019 06:01:23: 32000000 INFO @ Mon, 03 Jun 2019 06:01:24: 32000000 INFO @ Mon, 03 Jun 2019 06:01:25: 31000000 INFO @ Mon, 03 Jun 2019 06:01:31: 33000000 INFO @ Mon, 03 Jun 2019 06:01:32: 33000000 INFO @ Mon, 03 Jun 2019 06:01:32: 32000000 INFO @ Mon, 03 Jun 2019 06:01:39: 33000000 INFO @ Mon, 03 Jun 2019 06:01:39: 34000000 INFO @ Mon, 03 Jun 2019 06:01:40: 34000000 INFO @ Mon, 03 Jun 2019 06:01:46: 34000000 INFO @ Mon, 03 Jun 2019 06:01:47: 35000000 INFO @ Mon, 03 Jun 2019 06:01:47: 35000000 INFO @ Mon, 03 Jun 2019 06:01:53: 35000000 INFO @ Mon, 03 Jun 2019 06:01:55: 36000000 INFO @ Mon, 03 Jun 2019 06:01:55: 36000000 INFO @ Mon, 03 Jun 2019 06:01:58: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 06:01:58: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 06:01:58: #1 total tags in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:01:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 06:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 06:01:58: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 06:01:58: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 06:01:58: #1 total tags in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:01:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 06:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 06:01:58: #1 tags after filtering in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:01:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 06:01:58: #1 finished! INFO @ Mon, 03 Jun 2019 06:01:58: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 06:01:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 06:01:59: #1 tags after filtering in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 06:01:59: #1 finished! INFO @ Mon, 03 Jun 2019 06:01:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 06:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 06:02:00: 36000000 INFO @ Mon, 03 Jun 2019 06:02:01: #2 number of paired peaks: 48 WARNING @ Mon, 03 Jun 2019 06:02:01: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 06:02:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 06:02:02: #2 number of paired peaks: 48 WARNING @ Mon, 03 Jun 2019 06:02:02: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 06:02:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 06:02:03: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 06:02:03: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 06:02:03: #1 total tags in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:02:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 06:02:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 06:02:03: #1 tags after filtering in treatment: 36355011 INFO @ Mon, 03 Jun 2019 06:02:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 06:02:03: #1 finished! INFO @ Mon, 03 Jun 2019 06:02:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 06:02:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 06:02:06: #2 number of paired peaks: 48 WARNING @ Mon, 03 Jun 2019 06:02:06: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 06:02:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197585/SRX197585.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。