Job ID = 9158607 sra ファイルのダウンロード中... Completed: 1239479K bytes transferred in 13 seconds (743592K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 41989083 spots for /home/okishinya/chipatlas/results/dm3/SRX1847049/SRR3669866.sra Written 41989083 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:26 41989083 reads; of these: 41989083 (100.00%) were unpaired; of these: 1061560 (2.53%) aligned 0 times 35788477 (85.23%) aligned exactly 1 time 5139046 (12.24%) aligned >1 times 97.47% overall alignment rate Time searching: 00:12:26 Overall time: 00:12:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12580911 / 40927523 = 0.3074 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:54:58: # Command line: callpeak -t SRX1847049.bam -f BAM -g dm -n SRX1847049.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1847049.20 # format = BAM # ChIP-seq file = ['SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:54:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:54:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:54:58: # Command line: callpeak -t SRX1847049.bam -f BAM -g dm -n SRX1847049.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1847049.05 # format = BAM # ChIP-seq file = ['SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:54:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:54:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:54:58: # Command line: callpeak -t SRX1847049.bam -f BAM -g dm -n SRX1847049.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1847049.10 # format = BAM # ChIP-seq file = ['SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:54:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:54:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:55:05: 1000000 INFO @ Tue, 27 Jun 2017 18:55:05: 1000000 INFO @ Tue, 27 Jun 2017 18:55:05: 1000000 INFO @ Tue, 27 Jun 2017 18:55:12: 2000000 INFO @ Tue, 27 Jun 2017 18:55:12: 2000000 INFO @ Tue, 27 Jun 2017 18:55:13: 2000000 INFO @ Tue, 27 Jun 2017 18:55:20: 3000000 INFO @ Tue, 27 Jun 2017 18:55:20: 3000000 INFO @ Tue, 27 Jun 2017 18:55:21: 3000000 INFO @ Tue, 27 Jun 2017 18:55:28: 4000000 INFO @ Tue, 27 Jun 2017 18:55:28: 4000000 INFO @ Tue, 27 Jun 2017 18:55:29: 4000000 INFO @ Tue, 27 Jun 2017 18:55:36: 5000000 INFO @ Tue, 27 Jun 2017 18:55:36: 5000000 INFO @ Tue, 27 Jun 2017 18:55:37: 5000000 INFO @ Tue, 27 Jun 2017 18:55:44: 6000000 INFO @ Tue, 27 Jun 2017 18:55:44: 6000000 INFO @ Tue, 27 Jun 2017 18:55:45: 6000000 INFO @ Tue, 27 Jun 2017 18:55:52: 7000000 INFO @ Tue, 27 Jun 2017 18:55:52: 7000000 INFO @ Tue, 27 Jun 2017 18:55:54: 7000000 INFO @ Tue, 27 Jun 2017 18:56:01: 8000000 INFO @ Tue, 27 Jun 2017 18:56:01: 8000000 INFO @ Tue, 27 Jun 2017 18:56:02: 8000000 INFO @ Tue, 27 Jun 2017 18:56:09: 9000000 INFO @ Tue, 27 Jun 2017 18:56:09: 9000000 INFO @ Tue, 27 Jun 2017 18:56:10: 9000000 INFO @ Tue, 27 Jun 2017 18:56:17: 10000000 INFO @ Tue, 27 Jun 2017 18:56:17: 10000000 INFO @ Tue, 27 Jun 2017 18:56:18: 10000000 INFO @ Tue, 27 Jun 2017 18:56:25: 11000000 INFO @ Tue, 27 Jun 2017 18:56:25: 11000000 INFO @ Tue, 27 Jun 2017 18:56:26: 11000000 INFO @ Tue, 27 Jun 2017 18:56:33: 12000000 INFO @ Tue, 27 Jun 2017 18:56:33: 12000000 INFO @ Tue, 27 Jun 2017 18:56:35: 12000000 INFO @ Tue, 27 Jun 2017 18:56:41: 13000000 INFO @ Tue, 27 Jun 2017 18:56:41: 13000000 INFO @ Tue, 27 Jun 2017 18:56:43: 13000000 INFO @ Tue, 27 Jun 2017 18:56:49: 14000000 INFO @ Tue, 27 Jun 2017 18:56:49: 14000000 INFO @ Tue, 27 Jun 2017 18:56:51: 14000000 INFO @ Tue, 27 Jun 2017 18:56:57: 15000000 INFO @ Tue, 27 Jun 2017 18:56:57: 15000000 INFO @ Tue, 27 Jun 2017 18:56:59: 15000000 INFO @ Tue, 27 Jun 2017 18:57:06: 16000000 INFO @ Tue, 27 Jun 2017 18:57:06: 16000000 INFO @ Tue, 27 Jun 2017 18:57:08: 16000000 INFO @ Tue, 27 Jun 2017 18:57:14: 17000000 INFO @ Tue, 27 Jun 2017 18:57:14: 17000000 INFO @ Tue, 27 Jun 2017 18:57:16: 17000000 INFO @ Tue, 27 Jun 2017 18:57:22: 18000000 INFO @ Tue, 27 Jun 2017 18:57:22: 18000000 INFO @ Tue, 27 Jun 2017 18:57:24: 18000000 INFO @ Tue, 27 Jun 2017 18:57:30: 19000000 INFO @ Tue, 27 Jun 2017 18:57:30: 19000000 INFO @ Tue, 27 Jun 2017 18:57:32: 19000000 INFO @ Tue, 27 Jun 2017 18:57:38: 20000000 INFO @ Tue, 27 Jun 2017 18:57:38: 20000000 INFO @ Tue, 27 Jun 2017 18:57:40: 20000000 INFO @ Tue, 27 Jun 2017 18:57:46: 21000000 INFO @ Tue, 27 Jun 2017 18:57:46: 21000000 INFO @ Tue, 27 Jun 2017 18:57:48: 21000000 INFO @ Tue, 27 Jun 2017 18:57:54: 22000000 INFO @ Tue, 27 Jun 2017 18:57:54: 22000000 INFO @ Tue, 27 Jun 2017 18:57:56: 22000000 INFO @ Tue, 27 Jun 2017 18:58:02: 23000000 INFO @ Tue, 27 Jun 2017 18:58:02: 23000000 INFO @ Tue, 27 Jun 2017 18:58:04: 23000000 INFO @ Tue, 27 Jun 2017 18:58:10: 24000000 INFO @ Tue, 27 Jun 2017 18:58:10: 24000000 INFO @ Tue, 27 Jun 2017 18:58:11: 24000000 INFO @ Tue, 27 Jun 2017 18:58:17: 25000000 INFO @ Tue, 27 Jun 2017 18:58:17: 25000000 INFO @ Tue, 27 Jun 2017 18:58:18: 25000000 INFO @ Tue, 27 Jun 2017 18:58:23: 26000000 INFO @ Tue, 27 Jun 2017 18:58:23: 26000000 INFO @ Tue, 27 Jun 2017 18:58:25: 26000000 INFO @ Tue, 27 Jun 2017 18:58:29: 27000000 INFO @ Tue, 27 Jun 2017 18:58:30: 27000000 INFO @ Tue, 27 Jun 2017 18:58:31: 27000000 INFO @ Tue, 27 Jun 2017 18:58:35: 28000000 INFO @ Tue, 27 Jun 2017 18:58:36: 28000000 INFO @ Tue, 27 Jun 2017 18:58:37: 28000000 INFO @ Tue, 27 Jun 2017 18:58:38: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:58:38: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:58:38: #1 total tags in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:38: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:58:38: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:58:38: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:58:38: #1 total tags in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:38: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:58:38: #1 tags after filtering in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:58:38: #1 finished! INFO @ Tue, 27 Jun 2017 18:58:38: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:58:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:58:39: #1 tags after filtering in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:58:39: #1 finished! INFO @ Tue, 27 Jun 2017 18:58:39: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:58:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:58:39: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:58:39: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:58:39: #1 total tags in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:39: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:58:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:58:39: #1 tags after filtering in treatment: 28346612 INFO @ Tue, 27 Jun 2017 18:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:58:39: #1 finished! INFO @ Tue, 27 Jun 2017 18:58:39: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:58:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:58:40: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:58:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:58:40: Process for pairing-model is terminated! cat: SRX1847049.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847049.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:58:40: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:58:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:58:40: Process for pairing-model is terminated! cat: SRX1847049.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847049.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:58:41: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:58:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:58:41: Process for pairing-model is terminated! cat: SRX1847049.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847049.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847049.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。