Job ID = 9158601 sra ファイルのダウンロード中... Completed: 1681309K bytes transferred in 18 seconds (738944K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 39162401 spots for /home/okishinya/chipatlas/results/dm3/SRX1847045/SRR3669862.sra Written 39162401 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:22 39162401 reads; of these: 39162401 (100.00%) were unpaired; of these: 3097559 (7.91%) aligned 0 times 27233409 (69.54%) aligned exactly 1 time 8831433 (22.55%) aligned >1 times 92.09% overall alignment rate Time searching: 00:15:22 Overall time: 00:15:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12583097 / 36064842 = 0.3489 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:52:53: # Command line: callpeak -t SRX1847045.bam -f BAM -g dm -n SRX1847045.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1847045.20 # format = BAM # ChIP-seq file = ['SRX1847045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:52:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:52:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:52:53: # Command line: callpeak -t SRX1847045.bam -f BAM -g dm -n SRX1847045.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1847045.05 # format = BAM # ChIP-seq file = ['SRX1847045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:52:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:52:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:52:53: # Command line: callpeak -t SRX1847045.bam -f BAM -g dm -n SRX1847045.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1847045.10 # format = BAM # ChIP-seq file = ['SRX1847045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:52:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:52:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:52:59: 1000000 INFO @ Tue, 27 Jun 2017 18:52:59: 1000000 INFO @ Tue, 27 Jun 2017 18:53:00: 1000000 INFO @ Tue, 27 Jun 2017 18:53:06: 2000000 INFO @ Tue, 27 Jun 2017 18:53:07: 2000000 INFO @ Tue, 27 Jun 2017 18:53:07: 2000000 INFO @ Tue, 27 Jun 2017 18:53:12: 3000000 INFO @ Tue, 27 Jun 2017 18:53:14: 3000000 INFO @ Tue, 27 Jun 2017 18:53:15: 3000000 INFO @ Tue, 27 Jun 2017 18:53:19: 4000000 INFO @ Tue, 27 Jun 2017 18:53:21: 4000000 INFO @ Tue, 27 Jun 2017 18:53:22: 4000000 INFO @ Tue, 27 Jun 2017 18:53:25: 5000000 INFO @ Tue, 27 Jun 2017 18:53:28: 5000000 INFO @ Tue, 27 Jun 2017 18:53:29: 5000000 INFO @ Tue, 27 Jun 2017 18:53:32: 6000000 INFO @ Tue, 27 Jun 2017 18:53:34: 6000000 INFO @ Tue, 27 Jun 2017 18:53:37: 6000000 INFO @ Tue, 27 Jun 2017 18:53:39: 7000000 INFO @ Tue, 27 Jun 2017 18:53:41: 7000000 INFO @ Tue, 27 Jun 2017 18:53:44: 7000000 INFO @ Tue, 27 Jun 2017 18:53:46: 8000000 INFO @ Tue, 27 Jun 2017 18:53:48: 8000000 INFO @ Tue, 27 Jun 2017 18:53:51: 8000000 INFO @ Tue, 27 Jun 2017 18:53:53: 9000000 INFO @ Tue, 27 Jun 2017 18:53:54: 9000000 INFO @ Tue, 27 Jun 2017 18:53:58: 9000000 INFO @ Tue, 27 Jun 2017 18:54:00: 10000000 INFO @ Tue, 27 Jun 2017 18:54:01: 10000000 INFO @ Tue, 27 Jun 2017 18:54:06: 10000000 INFO @ Tue, 27 Jun 2017 18:54:07: 11000000 INFO @ Tue, 27 Jun 2017 18:54:07: 11000000 INFO @ Tue, 27 Jun 2017 18:54:13: 11000000 INFO @ Tue, 27 Jun 2017 18:54:13: 12000000 INFO @ Tue, 27 Jun 2017 18:54:14: 12000000 INFO @ Tue, 27 Jun 2017 18:54:20: 13000000 INFO @ Tue, 27 Jun 2017 18:54:20: 12000000 INFO @ Tue, 27 Jun 2017 18:54:21: 13000000 INFO @ Tue, 27 Jun 2017 18:54:26: 14000000 INFO @ Tue, 27 Jun 2017 18:54:27: 13000000 INFO @ Tue, 27 Jun 2017 18:54:28: 14000000 INFO @ Tue, 27 Jun 2017 18:54:33: 15000000 INFO @ Tue, 27 Jun 2017 18:54:35: 14000000 INFO @ Tue, 27 Jun 2017 18:54:35: 15000000 INFO @ Tue, 27 Jun 2017 18:54:39: 16000000 INFO @ Tue, 27 Jun 2017 18:54:42: 15000000 INFO @ Tue, 27 Jun 2017 18:54:43: 16000000 INFO @ Tue, 27 Jun 2017 18:54:45: 17000000 INFO @ Tue, 27 Jun 2017 18:54:50: 17000000 INFO @ Tue, 27 Jun 2017 18:54:50: 16000000 INFO @ Tue, 27 Jun 2017 18:54:52: 18000000 INFO @ Tue, 27 Jun 2017 18:54:57: 18000000 INFO @ Tue, 27 Jun 2017 18:54:57: 17000000 INFO @ Tue, 27 Jun 2017 18:54:58: 19000000 INFO @ Tue, 27 Jun 2017 18:55:04: 19000000 INFO @ Tue, 27 Jun 2017 18:55:05: 20000000 INFO @ Tue, 27 Jun 2017 18:55:05: 18000000 INFO @ Tue, 27 Jun 2017 18:55:11: 21000000 INFO @ Tue, 27 Jun 2017 18:55:12: 20000000 INFO @ Tue, 27 Jun 2017 18:55:12: 19000000 INFO @ Tue, 27 Jun 2017 18:55:17: 22000000 INFO @ Tue, 27 Jun 2017 18:55:19: 21000000 INFO @ Tue, 27 Jun 2017 18:55:20: 20000000 INFO @ Tue, 27 Jun 2017 18:55:24: 23000000 INFO @ Tue, 27 Jun 2017 18:55:26: 22000000 INFO @ Tue, 27 Jun 2017 18:55:27: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:55:27: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:55:27: #1 total tags in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:27: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:27: #1 tags after filtering in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:27: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:27: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:27: 21000000 INFO @ Tue, 27 Jun 2017 18:55:29: #2 number of paired peaks: 3 WARNING @ Tue, 27 Jun 2017 18:55:29: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:29: Process for pairing-model is terminated! cat: SRX1847045.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847045.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:55:33: 23000000 INFO @ Tue, 27 Jun 2017 18:55:35: 22000000 INFO @ Tue, 27 Jun 2017 18:55:37: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:55:37: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:55:37: #1 total tags in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:37: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:37: #1 tags after filtering in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:37: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:37: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:39: #2 number of paired peaks: 3 WARNING @ Tue, 27 Jun 2017 18:55:39: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:39: Process for pairing-model is terminated! cat: SRX1847045.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847045.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:55:41: 23000000 INFO @ Tue, 27 Jun 2017 18:55:45: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:55:45: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:55:45: #1 total tags in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:45: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:45: #1 tags after filtering in treatment: 23481745 INFO @ Tue, 27 Jun 2017 18:55:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:45: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:45: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:47: #2 number of paired peaks: 3 WARNING @ Tue, 27 Jun 2017 18:55:47: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:47: Process for pairing-model is terminated! cat: SRX1847045.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847045.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847045.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。