Job ID = 9158597 sra ファイルのダウンロード中... Completed: 1610207K bytes transferred in 18 seconds (723184K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 38009787 spots for /home/okishinya/chipatlas/results/dm3/SRX1847041/SRR3669858.sra Written 38009787 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:32 38009787 reads; of these: 38009787 (100.00%) were unpaired; of these: 695215 (1.83%) aligned 0 times 29660673 (78.03%) aligned exactly 1 time 7653899 (20.14%) aligned >1 times 98.17% overall alignment rate Time searching: 00:13:32 Overall time: 00:13:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11857877 / 37314572 = 0.3178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:50:01: # Command line: callpeak -t SRX1847041.bam -f BAM -g dm -n SRX1847041.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1847041.05 # format = BAM # ChIP-seq file = ['SRX1847041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:50:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:50:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:50:01: # Command line: callpeak -t SRX1847041.bam -f BAM -g dm -n SRX1847041.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1847041.20 # format = BAM # ChIP-seq file = ['SRX1847041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:50:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:50:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:50:01: # Command line: callpeak -t SRX1847041.bam -f BAM -g dm -n SRX1847041.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1847041.10 # format = BAM # ChIP-seq file = ['SRX1847041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:50:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:50:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:50:08: 1000000 INFO @ Tue, 27 Jun 2017 18:50:08: 1000000 INFO @ Tue, 27 Jun 2017 18:50:08: 1000000 INFO @ Tue, 27 Jun 2017 18:50:15: 2000000 INFO @ Tue, 27 Jun 2017 18:50:15: 2000000 INFO @ Tue, 27 Jun 2017 18:50:15: 2000000 INFO @ Tue, 27 Jun 2017 18:50:21: 3000000 INFO @ Tue, 27 Jun 2017 18:50:21: 3000000 INFO @ Tue, 27 Jun 2017 18:50:21: 3000000 INFO @ Tue, 27 Jun 2017 18:50:29: 4000000 INFO @ Tue, 27 Jun 2017 18:50:29: 4000000 INFO @ Tue, 27 Jun 2017 18:50:29: 4000000 INFO @ Tue, 27 Jun 2017 18:50:35: 5000000 INFO @ Tue, 27 Jun 2017 18:50:35: 5000000 INFO @ Tue, 27 Jun 2017 18:50:35: 5000000 INFO @ Tue, 27 Jun 2017 18:50:41: 6000000 INFO @ Tue, 27 Jun 2017 18:50:41: 6000000 INFO @ Tue, 27 Jun 2017 18:50:41: 6000000 INFO @ Tue, 27 Jun 2017 18:50:48: 7000000 INFO @ Tue, 27 Jun 2017 18:50:49: 7000000 INFO @ Tue, 27 Jun 2017 18:50:49: 7000000 INFO @ Tue, 27 Jun 2017 18:50:55: 8000000 INFO @ Tue, 27 Jun 2017 18:50:55: 8000000 INFO @ Tue, 27 Jun 2017 18:50:55: 8000000 INFO @ Tue, 27 Jun 2017 18:51:02: 9000000 INFO @ Tue, 27 Jun 2017 18:51:02: 9000000 INFO @ Tue, 27 Jun 2017 18:51:02: 9000000 INFO @ Tue, 27 Jun 2017 18:51:09: 10000000 INFO @ Tue, 27 Jun 2017 18:51:09: 10000000 INFO @ Tue, 27 Jun 2017 18:51:09: 10000000 INFO @ Tue, 27 Jun 2017 18:51:15: 11000000 INFO @ Tue, 27 Jun 2017 18:51:15: 11000000 INFO @ Tue, 27 Jun 2017 18:51:15: 11000000 INFO @ Tue, 27 Jun 2017 18:51:22: 12000000 INFO @ Tue, 27 Jun 2017 18:51:22: 12000000 INFO @ Tue, 27 Jun 2017 18:51:22: 12000000 INFO @ Tue, 27 Jun 2017 18:51:28: 13000000 INFO @ Tue, 27 Jun 2017 18:51:28: 13000000 INFO @ Tue, 27 Jun 2017 18:51:29: 13000000 INFO @ Tue, 27 Jun 2017 18:51:35: 14000000 INFO @ Tue, 27 Jun 2017 18:51:35: 14000000 INFO @ Tue, 27 Jun 2017 18:51:35: 14000000 INFO @ Tue, 27 Jun 2017 18:51:42: 15000000 INFO @ Tue, 27 Jun 2017 18:51:42: 15000000 INFO @ Tue, 27 Jun 2017 18:51:42: 15000000 INFO @ Tue, 27 Jun 2017 18:51:48: 16000000 INFO @ Tue, 27 Jun 2017 18:51:48: 16000000 INFO @ Tue, 27 Jun 2017 18:51:48: 16000000 INFO @ Tue, 27 Jun 2017 18:51:54: 17000000 INFO @ Tue, 27 Jun 2017 18:51:54: 17000000 INFO @ Tue, 27 Jun 2017 18:51:55: 17000000 INFO @ Tue, 27 Jun 2017 18:52:01: 18000000 INFO @ Tue, 27 Jun 2017 18:52:01: 18000000 INFO @ Tue, 27 Jun 2017 18:52:01: 18000000 INFO @ Tue, 27 Jun 2017 18:52:07: 19000000 INFO @ Tue, 27 Jun 2017 18:52:08: 19000000 INFO @ Tue, 27 Jun 2017 18:52:08: 19000000 INFO @ Tue, 27 Jun 2017 18:52:14: 20000000 INFO @ Tue, 27 Jun 2017 18:52:14: 20000000 INFO @ Tue, 27 Jun 2017 18:52:14: 20000000 INFO @ Tue, 27 Jun 2017 18:52:20: 21000000 INFO @ Tue, 27 Jun 2017 18:52:20: 21000000 INFO @ Tue, 27 Jun 2017 18:52:21: 21000000 INFO @ Tue, 27 Jun 2017 18:52:27: 22000000 INFO @ Tue, 27 Jun 2017 18:52:27: 22000000 INFO @ Tue, 27 Jun 2017 18:52:27: 22000000 INFO @ Tue, 27 Jun 2017 18:52:33: 23000000 INFO @ Tue, 27 Jun 2017 18:52:33: 23000000 INFO @ Tue, 27 Jun 2017 18:52:34: 23000000 INFO @ Tue, 27 Jun 2017 18:52:39: 24000000 INFO @ Tue, 27 Jun 2017 18:52:40: 24000000 INFO @ Tue, 27 Jun 2017 18:52:40: 24000000 INFO @ Tue, 27 Jun 2017 18:52:45: 25000000 INFO @ Tue, 27 Jun 2017 18:52:46: 25000000 INFO @ Tue, 27 Jun 2017 18:52:47: 25000000 INFO @ Tue, 27 Jun 2017 18:52:48: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:52:48: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:52:48: #1 total tags in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:52:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:52:49: #1 tags after filtering in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:52:49: #1 finished! INFO @ Tue, 27 Jun 2017 18:52:49: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:52:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:52:50: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:52:50: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:52:50: #1 total tags in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:50: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:52:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:52:50: #1 tags after filtering in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:52:50: #1 finished! INFO @ Tue, 27 Jun 2017 18:52:50: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:52:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:52:50: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:52:50: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:52:50: #1 total tags in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:50: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:52:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:52:51: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:52:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:52:51: Process for pairing-model is terminated! cat: SRX1847041.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847041.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:52:51: #1 tags after filtering in treatment: 25456695 INFO @ Tue, 27 Jun 2017 18:52:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:52:51: #1 finished! INFO @ Tue, 27 Jun 2017 18:52:51: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:52:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:52:52: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:52:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:52:52: Process for pairing-model is terminated! cat: SRX1847041.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847041.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:52:52: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:52:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:52:52: Process for pairing-model is terminated! cat: SRX1847041.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847041.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847041.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。