Job ID = 9157934 sra ファイルのダウンロード中... Completed: 895181K bytes transferred in 12 seconds (586465K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 37759110 spots for /home/okishinya/chipatlas/results/dm3/SRX1837297/SRR3657653.sra Written 37759110 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:37 37759110 reads; of these: 37759110 (100.00%) were unpaired; of these: 1149991 (3.05%) aligned 0 times 30578217 (80.98%) aligned exactly 1 time 6030902 (15.97%) aligned >1 times 96.95% overall alignment rate Time searching: 00:12:38 Overall time: 00:12:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5829676 / 36609119 = 0.1592 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 14:29:33: # Command line: callpeak -t SRX1837297.bam -f BAM -g dm -n SRX1837297.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1837297.10 # format = BAM # ChIP-seq file = ['SRX1837297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:29:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:29:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:29:33: # Command line: callpeak -t SRX1837297.bam -f BAM -g dm -n SRX1837297.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1837297.05 # format = BAM # ChIP-seq file = ['SRX1837297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:29:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:29:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:29:33: # Command line: callpeak -t SRX1837297.bam -f BAM -g dm -n SRX1837297.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1837297.20 # format = BAM # ChIP-seq file = ['SRX1837297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:29:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:29:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:29:41: 1000000 INFO @ Tue, 27 Jun 2017 14:29:41: 1000000 INFO @ Tue, 27 Jun 2017 14:29:42: 1000000 INFO @ Tue, 27 Jun 2017 14:29:49: 2000000 INFO @ Tue, 27 Jun 2017 14:29:49: 2000000 INFO @ Tue, 27 Jun 2017 14:29:50: 2000000 INFO @ Tue, 27 Jun 2017 14:29:57: 3000000 INFO @ Tue, 27 Jun 2017 14:29:57: 3000000 INFO @ Tue, 27 Jun 2017 14:29:58: 3000000 INFO @ Tue, 27 Jun 2017 14:30:05: 4000000 INFO @ Tue, 27 Jun 2017 14:30:05: 4000000 INFO @ Tue, 27 Jun 2017 14:30:05: 4000000 INFO @ Tue, 27 Jun 2017 14:30:13: 5000000 INFO @ Tue, 27 Jun 2017 14:30:13: 5000000 INFO @ Tue, 27 Jun 2017 14:30:13: 5000000 INFO @ Tue, 27 Jun 2017 14:30:21: 6000000 INFO @ Tue, 27 Jun 2017 14:30:21: 6000000 INFO @ Tue, 27 Jun 2017 14:30:21: 6000000 INFO @ Tue, 27 Jun 2017 14:30:29: 7000000 INFO @ Tue, 27 Jun 2017 14:30:29: 7000000 INFO @ Tue, 27 Jun 2017 14:30:29: 7000000 INFO @ Tue, 27 Jun 2017 14:30:36: 8000000 INFO @ Tue, 27 Jun 2017 14:30:36: 8000000 INFO @ Tue, 27 Jun 2017 14:30:38: 8000000 INFO @ Tue, 27 Jun 2017 14:30:44: 9000000 INFO @ Tue, 27 Jun 2017 14:30:44: 9000000 INFO @ Tue, 27 Jun 2017 14:30:46: 9000000 INFO @ Tue, 27 Jun 2017 14:30:52: 10000000 INFO @ Tue, 27 Jun 2017 14:30:52: 10000000 INFO @ Tue, 27 Jun 2017 14:30:54: 10000000 INFO @ Tue, 27 Jun 2017 14:31:00: 11000000 INFO @ Tue, 27 Jun 2017 14:31:00: 11000000 INFO @ Tue, 27 Jun 2017 14:31:02: 11000000 INFO @ Tue, 27 Jun 2017 14:31:07: 12000000 INFO @ Tue, 27 Jun 2017 14:31:07: 12000000 INFO @ Tue, 27 Jun 2017 14:31:09: 12000000 INFO @ Tue, 27 Jun 2017 14:31:15: 13000000 INFO @ Tue, 27 Jun 2017 14:31:15: 13000000 INFO @ Tue, 27 Jun 2017 14:31:17: 13000000 INFO @ Tue, 27 Jun 2017 14:31:22: 14000000 INFO @ Tue, 27 Jun 2017 14:31:22: 14000000 INFO @ Tue, 27 Jun 2017 14:31:25: 14000000 INFO @ Tue, 27 Jun 2017 14:31:30: 15000000 INFO @ Tue, 27 Jun 2017 14:31:30: 15000000 INFO @ Tue, 27 Jun 2017 14:31:32: 15000000 INFO @ Tue, 27 Jun 2017 14:31:38: 16000000 INFO @ Tue, 27 Jun 2017 14:31:38: 16000000 INFO @ Tue, 27 Jun 2017 14:31:40: 16000000 INFO @ Tue, 27 Jun 2017 14:31:45: 17000000 INFO @ Tue, 27 Jun 2017 14:31:45: 17000000 INFO @ Tue, 27 Jun 2017 14:31:47: 17000000 INFO @ Tue, 27 Jun 2017 14:31:53: 18000000 INFO @ Tue, 27 Jun 2017 14:31:53: 18000000 INFO @ Tue, 27 Jun 2017 14:31:55: 18000000 INFO @ Tue, 27 Jun 2017 14:32:01: 19000000 INFO @ Tue, 27 Jun 2017 14:32:01: 19000000 INFO @ Tue, 27 Jun 2017 14:32:02: 19000000 INFO @ Tue, 27 Jun 2017 14:32:08: 20000000 INFO @ Tue, 27 Jun 2017 14:32:08: 20000000 INFO @ Tue, 27 Jun 2017 14:32:10: 20000000 INFO @ Tue, 27 Jun 2017 14:32:16: 21000000 INFO @ Tue, 27 Jun 2017 14:32:16: 21000000 INFO @ Tue, 27 Jun 2017 14:32:17: 21000000 INFO @ Tue, 27 Jun 2017 14:32:24: 22000000 INFO @ Tue, 27 Jun 2017 14:32:24: 22000000 INFO @ Tue, 27 Jun 2017 14:32:25: 22000000 INFO @ Tue, 27 Jun 2017 14:32:31: 23000000 INFO @ Tue, 27 Jun 2017 14:32:31: 23000000 INFO @ Tue, 27 Jun 2017 14:32:32: 23000000 INFO @ Tue, 27 Jun 2017 14:32:39: 24000000 INFO @ Tue, 27 Jun 2017 14:32:39: 24000000 INFO @ Tue, 27 Jun 2017 14:32:40: 24000000 INFO @ Tue, 27 Jun 2017 14:32:47: 25000000 INFO @ Tue, 27 Jun 2017 14:32:47: 25000000 INFO @ Tue, 27 Jun 2017 14:32:48: 25000000 INFO @ Tue, 27 Jun 2017 14:32:54: 26000000 INFO @ Tue, 27 Jun 2017 14:32:54: 26000000 INFO @ Tue, 27 Jun 2017 14:32:55: 26000000 INFO @ Tue, 27 Jun 2017 14:33:02: 27000000 INFO @ Tue, 27 Jun 2017 14:33:02: 27000000 INFO @ Tue, 27 Jun 2017 14:33:03: 27000000 INFO @ Tue, 27 Jun 2017 14:33:10: 28000000 INFO @ Tue, 27 Jun 2017 14:33:10: 28000000 INFO @ Tue, 27 Jun 2017 14:33:11: 28000000 INFO @ Tue, 27 Jun 2017 14:33:17: 29000000 INFO @ Tue, 27 Jun 2017 14:33:17: 29000000 INFO @ Tue, 27 Jun 2017 14:33:18: 29000000 INFO @ Tue, 27 Jun 2017 14:33:25: 30000000 INFO @ Tue, 27 Jun 2017 14:33:25: 30000000 INFO @ Tue, 27 Jun 2017 14:33:27: 30000000 INFO @ Tue, 27 Jun 2017 14:33:31: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 14:33:31: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 14:33:31: #1 total tags in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:33:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:33:31: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 14:33:31: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 14:33:31: #1 total tags in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:33:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:33:32: #1 tags after filtering in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:33:32: #1 finished! INFO @ Tue, 27 Jun 2017 14:33:32: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:33:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:33:32: #1 tags after filtering in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:33:32: #1 finished! INFO @ Tue, 27 Jun 2017 14:33:32: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:33:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:33:33: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 14:33:33: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 14:33:33: #1 total tags in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:33: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:33:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:33:34: #1 tags after filtering in treatment: 30779443 INFO @ Tue, 27 Jun 2017 14:33:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:33:34: #1 finished! INFO @ Tue, 27 Jun 2017 14:33:34: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:33:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:33:34: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 14:33:34: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:33:34: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 14:33:34: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 14:33:34: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:33:34: Process for pairing-model is terminated! cat: SRX1837297.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1837297.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1837297.05_model.r'rm: cannot remove `SRX1837297.10_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 14:33:36: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 14:33:36: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:33:36: Process for pairing-model is terminated! cat: SRX1837297.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1837297.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1837297.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。