Job ID = 6527634 SRX = SRX1743170 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:21:38 prefetch.2.10.7: 1) Downloading 'SRR3476581'... 2020-06-29T13:21:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:22:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:22:42 prefetch.2.10.7: 'SRR3476581' is valid 2020-06-29T13:22:42 prefetch.2.10.7: 1) 'SRR3476581' was downloaded successfully Read 3798746 spots for SRR3476581/SRR3476581.sra Written 3798746 spots for SRR3476581/SRR3476581.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:57 3798746 reads; of these: 3798746 (100.00%) were unpaired; of these: 1767923 (46.54%) aligned 0 times 1731283 (45.58%) aligned exactly 1 time 299540 (7.89%) aligned >1 times 53.46% overall alignment rate Time searching: 00:00:57 Overall time: 00:00:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 125424 / 2030823 = 0.0618 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:26:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:26:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:26:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:26:30: 1000000 INFO @ Mon, 29 Jun 2020 22:26:37: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:26:37: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:26:37: #1 total tags in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:26:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:26:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:26:37: #1 tags after filtering in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:26:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:26:37: #1 finished! INFO @ Mon, 29 Jun 2020 22:26:37: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:26:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:26:37: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 22:26:37: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:26:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:26:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:26:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:26:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:27:00: 1000000 INFO @ Mon, 29 Jun 2020 22:27:08: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:27:08: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:27:08: #1 total tags in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:27:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:27:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:27:08: #1 tags after filtering in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:27:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:27:08: #1 finished! INFO @ Mon, 29 Jun 2020 22:27:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:27:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:27:08: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 22:27:08: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:27:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:27:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:27:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:27:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:27:31: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 22:27:38: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:27:38: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:27:38: #1 total tags in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:27:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:27:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:27:38: #1 tags after filtering in treatment: 1905399 INFO @ Mon, 29 Jun 2020 22:27:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:27:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:27:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:27:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:27:39: #2 number of paired peaks: 75 WARNING @ Mon, 29 Jun 2020 22:27:39: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:27:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1743170/SRX1743170.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling