Job ID = 9158561 sra ファイルのダウンロード中... Completed: 1118706K bytes transferred in 11 seconds (772298K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 26463516 spots for /home/okishinya/chipatlas/results/dm3/SRX1715681/SRR3405222.sra Written 26463516 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:04 26463516 reads; of these: 26463516 (100.00%) were unpaired; of these: 894198 (3.38%) aligned 0 times 20937540 (79.12%) aligned exactly 1 time 4631778 (17.50%) aligned >1 times 96.62% overall alignment rate Time searching: 00:09:04 Overall time: 00:09:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6608087 / 25569318 = 0.2584 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:09:33: # Command line: callpeak -t SRX1715681.bam -f BAM -g dm -n SRX1715681.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1715681.10 # format = BAM # ChIP-seq file = ['SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:09:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:09:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:09:33: # Command line: callpeak -t SRX1715681.bam -f BAM -g dm -n SRX1715681.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1715681.05 # format = BAM # ChIP-seq file = ['SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:09:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:09:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:09:33: # Command line: callpeak -t SRX1715681.bam -f BAM -g dm -n SRX1715681.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1715681.20 # format = BAM # ChIP-seq file = ['SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:09:33: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:09:33: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:09:41: 1000000 INFO @ Tue, 27 Jun 2017 18:09:42: 1000000 INFO @ Tue, 27 Jun 2017 18:09:43: 1000000 INFO @ Tue, 27 Jun 2017 18:09:48: 2000000 INFO @ Tue, 27 Jun 2017 18:09:52: 2000000 INFO @ Tue, 27 Jun 2017 18:09:53: 2000000 INFO @ Tue, 27 Jun 2017 18:09:56: 3000000 INFO @ Tue, 27 Jun 2017 18:10:01: 3000000 INFO @ Tue, 27 Jun 2017 18:10:03: 4000000 INFO @ Tue, 27 Jun 2017 18:10:03: 3000000 INFO @ Tue, 27 Jun 2017 18:10:10: 4000000 INFO @ Tue, 27 Jun 2017 18:10:11: 5000000 INFO @ Tue, 27 Jun 2017 18:10:13: 4000000 INFO @ Tue, 27 Jun 2017 18:10:18: 6000000 INFO @ Tue, 27 Jun 2017 18:10:19: 5000000 INFO @ Tue, 27 Jun 2017 18:10:23: 5000000 INFO @ Tue, 27 Jun 2017 18:10:25: 7000000 INFO @ Tue, 27 Jun 2017 18:10:28: 6000000 INFO @ Tue, 27 Jun 2017 18:10:33: 8000000 INFO @ Tue, 27 Jun 2017 18:10:33: 6000000 INFO @ Tue, 27 Jun 2017 18:10:37: 7000000 INFO @ Tue, 27 Jun 2017 18:10:40: 9000000 INFO @ Tue, 27 Jun 2017 18:10:43: 7000000 INFO @ Tue, 27 Jun 2017 18:10:47: 8000000 INFO @ Tue, 27 Jun 2017 18:10:49: 10000000 INFO @ Tue, 27 Jun 2017 18:10:53: 8000000 INFO @ Tue, 27 Jun 2017 18:10:56: 9000000 INFO @ Tue, 27 Jun 2017 18:10:57: 11000000 INFO @ Tue, 27 Jun 2017 18:11:03: 9000000 INFO @ Tue, 27 Jun 2017 18:11:05: 10000000 INFO @ Tue, 27 Jun 2017 18:11:06: 12000000 INFO @ Tue, 27 Jun 2017 18:11:14: 10000000 INFO @ Tue, 27 Jun 2017 18:11:14: 11000000 INFO @ Tue, 27 Jun 2017 18:11:17: 13000000 INFO @ Tue, 27 Jun 2017 18:11:24: 12000000 INFO @ Tue, 27 Jun 2017 18:11:25: 11000000 INFO @ Tue, 27 Jun 2017 18:11:28: 14000000 INFO @ Tue, 27 Jun 2017 18:11:34: 13000000 INFO @ Tue, 27 Jun 2017 18:11:36: 12000000 INFO @ Tue, 27 Jun 2017 18:11:39: 15000000 INFO @ Tue, 27 Jun 2017 18:11:43: 14000000 INFO @ Tue, 27 Jun 2017 18:11:47: 13000000 INFO @ Tue, 27 Jun 2017 18:11:50: 16000000 INFO @ Tue, 27 Jun 2017 18:11:53: 15000000 INFO @ Tue, 27 Jun 2017 18:11:58: 14000000 INFO @ Tue, 27 Jun 2017 18:12:01: 17000000 INFO @ Tue, 27 Jun 2017 18:12:03: 16000000 INFO @ Tue, 27 Jun 2017 18:12:09: 15000000 INFO @ Tue, 27 Jun 2017 18:12:12: 18000000 INFO @ Tue, 27 Jun 2017 18:12:12: 17000000 INFO @ Tue, 27 Jun 2017 18:12:20: 16000000 INFO @ Tue, 27 Jun 2017 18:12:22: 18000000 INFO @ Tue, 27 Jun 2017 18:12:22: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:12:22: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:12:22: #1 total tags in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:22: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:12:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:12:22: #1 tags after filtering in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:12:22: #1 finished! INFO @ Tue, 27 Jun 2017 18:12:22: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:12:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:12:24: #2 number of paired peaks: 19 WARNING @ Tue, 27 Jun 2017 18:12:24: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:12:24: Process for pairing-model is terminated! cat: SRX1715681.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715681.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:12:30: 17000000 INFO @ Tue, 27 Jun 2017 18:12:30: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:12:30: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:12:30: #1 total tags in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:12:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:12:31: #1 tags after filtering in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:12:31: #1 finished! INFO @ Tue, 27 Jun 2017 18:12:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:12:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:12:32: #2 number of paired peaks: 19 WARNING @ Tue, 27 Jun 2017 18:12:32: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:12:32: Process for pairing-model is terminated! cat: SRX1715681.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715681.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:12:39: 18000000 INFO @ Tue, 27 Jun 2017 18:12:47: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:12:47: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:12:47: #1 total tags in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:12:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:12:47: #1 tags after filtering in treatment: 18961231 INFO @ Tue, 27 Jun 2017 18:12:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:12:47: #1 finished! INFO @ Tue, 27 Jun 2017 18:12:47: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:12:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:12:48: #2 number of paired peaks: 19 WARNING @ Tue, 27 Jun 2017 18:12:48: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:12:48: Process for pairing-model is terminated! cat: SRX1715681.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715681.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715681.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。