Job ID = 9158553 sra ファイルのダウンロード中... Completed: 1496033K bytes transferred in 14 seconds (867886K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 36067443 spots for /home/okishinya/chipatlas/results/dm3/SRX1715674/SRR3405215.sra Written 36067443 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:24 36067443 reads; of these: 36067443 (100.00%) were unpaired; of these: 847028 (2.35%) aligned 0 times 32124010 (89.07%) aligned exactly 1 time 3096405 (8.59%) aligned >1 times 97.65% overall alignment rate Time searching: 00:11:24 Overall time: 00:11:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12677032 / 35220415 = 0.3599 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:12:21: # Command line: callpeak -t SRX1715674.bam -f BAM -g dm -n SRX1715674.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1715674.05 # format = BAM # ChIP-seq file = ['SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:12:21: # Command line: callpeak -t SRX1715674.bam -f BAM -g dm -n SRX1715674.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1715674.20 # format = BAM # ChIP-seq file = ['SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:12:21: # Command line: callpeak -t SRX1715674.bam -f BAM -g dm -n SRX1715674.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1715674.10 # format = BAM # ChIP-seq file = ['SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:12:21: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:12:21: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:12:28: 1000000 INFO @ Tue, 27 Jun 2017 18:12:28: 1000000 INFO @ Tue, 27 Jun 2017 18:12:28: 1000000 INFO @ Tue, 27 Jun 2017 18:12:35: 2000000 INFO @ Tue, 27 Jun 2017 18:12:35: 2000000 INFO @ Tue, 27 Jun 2017 18:12:35: 2000000 INFO @ Tue, 27 Jun 2017 18:12:41: 3000000 INFO @ Tue, 27 Jun 2017 18:12:42: 3000000 INFO @ Tue, 27 Jun 2017 18:12:42: 3000000 INFO @ Tue, 27 Jun 2017 18:12:48: 4000000 INFO @ Tue, 27 Jun 2017 18:12:48: 4000000 INFO @ Tue, 27 Jun 2017 18:12:48: 4000000 INFO @ Tue, 27 Jun 2017 18:12:55: 5000000 INFO @ Tue, 27 Jun 2017 18:12:55: 5000000 INFO @ Tue, 27 Jun 2017 18:12:55: 5000000 INFO @ Tue, 27 Jun 2017 18:13:01: 6000000 INFO @ Tue, 27 Jun 2017 18:13:02: 6000000 INFO @ Tue, 27 Jun 2017 18:13:02: 6000000 INFO @ Tue, 27 Jun 2017 18:13:08: 7000000 INFO @ Tue, 27 Jun 2017 18:13:09: 7000000 INFO @ Tue, 27 Jun 2017 18:13:09: 7000000 INFO @ Tue, 27 Jun 2017 18:13:14: 8000000 INFO @ Tue, 27 Jun 2017 18:13:16: 8000000 INFO @ Tue, 27 Jun 2017 18:13:16: 8000000 INFO @ Tue, 27 Jun 2017 18:13:21: 9000000 INFO @ Tue, 27 Jun 2017 18:13:23: 9000000 INFO @ Tue, 27 Jun 2017 18:13:23: 9000000 INFO @ Tue, 27 Jun 2017 18:13:29: 10000000 INFO @ Tue, 27 Jun 2017 18:13:31: 10000000 INFO @ Tue, 27 Jun 2017 18:13:31: 10000000 INFO @ Tue, 27 Jun 2017 18:13:37: 11000000 INFO @ Tue, 27 Jun 2017 18:13:39: 11000000 INFO @ Tue, 27 Jun 2017 18:13:39: 11000000 INFO @ Tue, 27 Jun 2017 18:13:45: 12000000 INFO @ Tue, 27 Jun 2017 18:13:47: 12000000 INFO @ Tue, 27 Jun 2017 18:13:47: 12000000 INFO @ Tue, 27 Jun 2017 18:13:53: 13000000 INFO @ Tue, 27 Jun 2017 18:13:55: 13000000 INFO @ Tue, 27 Jun 2017 18:13:55: 13000000 INFO @ Tue, 27 Jun 2017 18:14:01: 14000000 INFO @ Tue, 27 Jun 2017 18:14:03: 14000000 INFO @ Tue, 27 Jun 2017 18:14:03: 14000000 INFO @ Tue, 27 Jun 2017 18:14:09: 15000000 INFO @ Tue, 27 Jun 2017 18:14:10: 15000000 INFO @ Tue, 27 Jun 2017 18:14:10: 15000000 INFO @ Tue, 27 Jun 2017 18:14:17: 16000000 INFO @ Tue, 27 Jun 2017 18:14:18: 16000000 INFO @ Tue, 27 Jun 2017 18:14:18: 16000000 INFO @ Tue, 27 Jun 2017 18:14:25: 17000000 INFO @ Tue, 27 Jun 2017 18:14:26: 17000000 INFO @ Tue, 27 Jun 2017 18:14:26: 17000000 INFO @ Tue, 27 Jun 2017 18:14:33: 18000000 INFO @ Tue, 27 Jun 2017 18:14:34: 18000000 INFO @ Tue, 27 Jun 2017 18:14:34: 18000000 INFO @ Tue, 27 Jun 2017 18:14:41: 19000000 INFO @ Tue, 27 Jun 2017 18:14:42: 19000000 INFO @ Tue, 27 Jun 2017 18:14:42: 19000000 INFO @ Tue, 27 Jun 2017 18:14:49: 20000000 INFO @ Tue, 27 Jun 2017 18:14:50: 20000000 INFO @ Tue, 27 Jun 2017 18:14:50: 20000000 INFO @ Tue, 27 Jun 2017 18:14:57: 21000000 INFO @ Tue, 27 Jun 2017 18:14:57: 21000000 INFO @ Tue, 27 Jun 2017 18:14:57: 21000000 INFO @ Tue, 27 Jun 2017 18:15:05: 22000000 INFO @ Tue, 27 Jun 2017 18:15:05: 22000000 INFO @ Tue, 27 Jun 2017 18:15:05: 22000000 INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:15:10: #1 total tags in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 total tags in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:15:10: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:15:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:15:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:15:10: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:15:10: #1 total tags in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:15:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:15:10: #1 tags after filtering in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 tags after filtering in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:15:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:15:10: #1 finished! INFO @ Tue, 27 Jun 2017 18:15:10: #1 finished! INFO @ Tue, 27 Jun 2017 18:15:10: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:15:10: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:15:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:15:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:15:10: #1 tags after filtering in treatment: 22543383 INFO @ Tue, 27 Jun 2017 18:15:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:15:10: #1 finished! INFO @ Tue, 27 Jun 2017 18:15:10: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:15:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:15:11: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 18:15:11: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:15:11: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 18:15:11: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 18:15:11: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:15:11: Process for pairing-model is terminated! cat: SRX1715674.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1715674.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184)pass1 - making usageList (0 chroms) : 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715674.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.05_model.r': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX1715674.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:15:12: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 18:15:12: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:15:12: Process for pairing-model is terminated! cat: SRX1715674.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715674.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715674.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。