Job ID = 9158552 sra ファイルのダウンロード中... Completed: 1703334K bytes transferred in 15 seconds (884437K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 39547747 spots for /home/okishinya/chipatlas/results/dm3/SRX1715672/SRR3405213.sra Written 39547747 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:26 39547747 reads; of these: 39547747 (100.00%) were unpaired; of these: 842520 (2.13%) aligned 0 times 33319326 (84.25%) aligned exactly 1 time 5385901 (13.62%) aligned >1 times 97.87% overall alignment rate Time searching: 00:13:26 Overall time: 00:13:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14150748 / 38705227 = 0.3656 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:15:14: # Command line: callpeak -t SRX1715672.bam -f BAM -g dm -n SRX1715672.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1715672.05 # format = BAM # ChIP-seq file = ['SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:15:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:15:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:15:14: # Command line: callpeak -t SRX1715672.bam -f BAM -g dm -n SRX1715672.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1715672.10 # format = BAM # ChIP-seq file = ['SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:15:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:15:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:15:14: # Command line: callpeak -t SRX1715672.bam -f BAM -g dm -n SRX1715672.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1715672.20 # format = BAM # ChIP-seq file = ['SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:15:14: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:15:14: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:15:21: 1000000 INFO @ Tue, 27 Jun 2017 18:15:21: 1000000 INFO @ Tue, 27 Jun 2017 18:15:22: 1000000 INFO @ Tue, 27 Jun 2017 18:15:28: 2000000 INFO @ Tue, 27 Jun 2017 18:15:29: 2000000 INFO @ Tue, 27 Jun 2017 18:15:30: 2000000 INFO @ Tue, 27 Jun 2017 18:15:36: 3000000 INFO @ Tue, 27 Jun 2017 18:15:37: 3000000 INFO @ Tue, 27 Jun 2017 18:15:39: 3000000 INFO @ Tue, 27 Jun 2017 18:15:43: 4000000 INFO @ Tue, 27 Jun 2017 18:15:45: 4000000 INFO @ Tue, 27 Jun 2017 18:15:47: 4000000 INFO @ Tue, 27 Jun 2017 18:15:51: 5000000 INFO @ Tue, 27 Jun 2017 18:15:54: 5000000 INFO @ Tue, 27 Jun 2017 18:15:56: 5000000 INFO @ Tue, 27 Jun 2017 18:15:58: 6000000 INFO @ Tue, 27 Jun 2017 18:16:02: 6000000 INFO @ Tue, 27 Jun 2017 18:16:05: 6000000 INFO @ Tue, 27 Jun 2017 18:16:06: 7000000 INFO @ Tue, 27 Jun 2017 18:16:11: 7000000 INFO @ Tue, 27 Jun 2017 18:16:13: 8000000 INFO @ Tue, 27 Jun 2017 18:16:13: 7000000 INFO @ Tue, 27 Jun 2017 18:16:20: 8000000 INFO @ Tue, 27 Jun 2017 18:16:21: 9000000 INFO @ Tue, 27 Jun 2017 18:16:22: 8000000 INFO @ Tue, 27 Jun 2017 18:16:28: 10000000 INFO @ Tue, 27 Jun 2017 18:16:28: 9000000 INFO @ Tue, 27 Jun 2017 18:16:30: 9000000 INFO @ Tue, 27 Jun 2017 18:16:35: 11000000 INFO @ Tue, 27 Jun 2017 18:16:36: 10000000 INFO @ Tue, 27 Jun 2017 18:16:38: 10000000 INFO @ Tue, 27 Jun 2017 18:16:42: 12000000 INFO @ Tue, 27 Jun 2017 18:16:45: 11000000 INFO @ Tue, 27 Jun 2017 18:16:46: 11000000 INFO @ Tue, 27 Jun 2017 18:16:49: 13000000 INFO @ Tue, 27 Jun 2017 18:16:53: 12000000 INFO @ Tue, 27 Jun 2017 18:16:54: 12000000 INFO @ Tue, 27 Jun 2017 18:16:56: 14000000 INFO @ Tue, 27 Jun 2017 18:17:01: 13000000 INFO @ Tue, 27 Jun 2017 18:17:02: 13000000 INFO @ Tue, 27 Jun 2017 18:17:03: 15000000 INFO @ Tue, 27 Jun 2017 18:17:09: 14000000 INFO @ Tue, 27 Jun 2017 18:17:10: 14000000 INFO @ Tue, 27 Jun 2017 18:17:11: 16000000 INFO @ Tue, 27 Jun 2017 18:17:17: 15000000 INFO @ Tue, 27 Jun 2017 18:17:18: 17000000 INFO @ Tue, 27 Jun 2017 18:17:18: 15000000 INFO @ Tue, 27 Jun 2017 18:17:25: 18000000 INFO @ Tue, 27 Jun 2017 18:17:26: 16000000 INFO @ Tue, 27 Jun 2017 18:17:27: 16000000 INFO @ Tue, 27 Jun 2017 18:17:32: 19000000 INFO @ Tue, 27 Jun 2017 18:17:34: 17000000 INFO @ Tue, 27 Jun 2017 18:17:35: 17000000 INFO @ Tue, 27 Jun 2017 18:17:39: 20000000 INFO @ Tue, 27 Jun 2017 18:17:42: 18000000 INFO @ Tue, 27 Jun 2017 18:17:43: 18000000 INFO @ Tue, 27 Jun 2017 18:17:46: 21000000 INFO @ Tue, 27 Jun 2017 18:17:50: 19000000 INFO @ Tue, 27 Jun 2017 18:17:51: 19000000 INFO @ Tue, 27 Jun 2017 18:17:53: 22000000 INFO @ Tue, 27 Jun 2017 18:17:58: 20000000 INFO @ Tue, 27 Jun 2017 18:17:59: 20000000 INFO @ Tue, 27 Jun 2017 18:18:00: 23000000 INFO @ Tue, 27 Jun 2017 18:18:06: 21000000 INFO @ Tue, 27 Jun 2017 18:18:07: 21000000 INFO @ Tue, 27 Jun 2017 18:18:07: 24000000 INFO @ Tue, 27 Jun 2017 18:18:12: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:18:12: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:18:12: #1 total tags in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:18:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:18:12: #1 tags after filtering in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:18:12: #1 finished! INFO @ Tue, 27 Jun 2017 18:18:12: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:18:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:18:14: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 18:18:14: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:18:14: Process for pairing-model is terminated! cat: SRX1715672.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715672.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:18:14: 22000000 INFO @ Tue, 27 Jun 2017 18:18:14: 22000000 INFO @ Tue, 27 Jun 2017 18:18:20: 23000000 INFO @ Tue, 27 Jun 2017 18:18:20: 23000000 INFO @ Tue, 27 Jun 2017 18:18:27: 24000000 INFO @ Tue, 27 Jun 2017 18:18:27: 24000000 INFO @ Tue, 27 Jun 2017 18:18:31: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:18:31: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:18:31: #1 total tags in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:18:31: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:18:31: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:18:31: #1 total tags in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:18:31: #1 tags after filtering in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:18:31: #1 finished! INFO @ Tue, 27 Jun 2017 18:18:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:18:32: #1 tags after filtering in treatment: 24554479 INFO @ Tue, 27 Jun 2017 18:18:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:18:32: #1 finished! INFO @ Tue, 27 Jun 2017 18:18:32: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:18:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:18:33: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 18:18:33: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:18:33: Process for pairing-model is terminated! cat: SRX1715672.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715672.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:18:33: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 18:18:33: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:18:33: Process for pairing-model is terminated! cat: SRX1715672.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1715672.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1715672.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。